Serum-free, protein-free and DMSO-free cell freezing medium and application thereof

Abstract

The invention discloses a serum-free, protein-free and DMSO-free cell freezing medium and application thereof. The invention firstly discloses the serum-free, protein-free and DMSO-free cell freezing medium. The serum-free, protein-free and DMSO-free cell freezing medium comprises the following components: a glucose injection, a compound amino acid injection, glycerol, monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, sucrose, hydroxyethyl starch, adenosine triphosphate-disodium magnesium chloride and water. The invention further discloses application of the cell freezing medium in cryopreservation of cells. The cell freezing medium is clear in component and stable in batch, and meets the requirement of industrial transformation. Besides, the cell freezing medium is free of serum, protein and DMSO, the activity and dryness of the cells can be stably maintained for a long time when the cells are cryopreserved, the recovered cells can be directly used for clinical application, and the safety requirement and the effectiveness requirement of the clinical application are met.

Description

technical field [0001] The invention relates to the field of cell cryopreservation. More specifically, it relates to a serum-free, protein-free and DMSO-free cell cryopreservation solution and its application. Background technique [0002] At present, cell therapy is more and more widely used in clinical practice. In particular, stem cell therapy has great advantages and broad development prospects in the treatment of degenerative, traumatic, genetic defect and other diseases (Ella Buzhor, Lucy, Leshansky, Jacob Blumenthal, et al. Cell-based therapy approaches: the hope for incurable diseases. Regenerative Medicine. (2014) 9(5), 649–672). Efficient and safe cell preparations are the key factors in cell therapy. Traditional cell preparations consist of a mixture of living cells and an electrolyte solution (such as Bomalis A). After the preparation is completed, it generally needs to be reinfused within 12 hours, and the cell viability will decrease after 12 hours, which w...

AI Technical Summary

Problems solved by technology

Although the incidence of toxic and side effects can be reduced by washing, the washing process will lead to a significant reduction in the number of cells, especially the stem cells themselves, and further loss may lead to treatment failure due to insufficient transplanted cells

In addition, washing the cells after recovery is not only cumbersome but also increases the risk of cell contamination

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