Arsenic Fluorescent Quantum Dots, Preparation Method and Application thereof

A technology of fluorescent quantum dots and molecular formulas, applied in the field of nanomaterials and nanobiology, can solve the problems of loss of tracer function, separation of drugs and fluorescent substances, etc., achieve maximum curative effect, simple and controllable preparation process, and enhance the effect of fluorescence efficiency

Active Publication Date: 2022-08-02
湖南中医药高等专科学校
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the nano-drug delivery system that combines drugs with fluorescent substances also has defects. Drugs and fluorescent substances may be separated in the body, losing the tracking function

Method used

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  • Arsenic Fluorescent Quantum Dots, Preparation Method and Application thereof
  • Arsenic Fluorescent Quantum Dots, Preparation Method and Application thereof
  • Arsenic Fluorescent Quantum Dots, Preparation Method and Application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of arsenic fluorescent quantum dots:

[0038] The realgar was dissolved in ethylenediamine, sonicated for 15 min, centrifuged at 15000 rpm for 5 min, and the supernatant was taken. Disperse 1.2mL of supernatant in 8mL of water, stir for 5min, add 2.5mL of HA aqueous solution (40mg / mL), stir for 5min, add 4.8mL of 4M HCl, stir for 10min, then place the mixed solution in a 250mL three-necked flask, 75 ℃ oil Bath for 72h to obtain crude product. Finally, the centrifugation supernatant was collected and separated by Sephadex column chromatography to obtain purified arsenic fluorescent quantum dots.

Embodiment 2

[0040] The fluorescence intensity of the arsenic fluorescent quantum dots formed in different oil bath times in Example 1 was investigated.

[0041] In the preparation method of Example 1, the 75°C oil bath was gradually extended from 12h to 72h, and the figure 2 It can be seen that with the prolongation of the oil bath time, the fluorescence intensity gradually increased until the equilibrium was reached at 72h.

Embodiment 3

[0043] The fluorescence intensity of arsenic fluorescent quantum dots under different excitation wavelengths was investigated.

[0044] Take As 4 S 4 The fluorescence emission spectra of @HA QDs were scanned at different excitation wavelengths, and the fluorescence spectra were obtained by image 3 It is known that As 4 S 4 The @HA QDs exhibited strong fluorescence at excitation wavelengths from 350nm to 410nm.

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Abstract

The invention provides an arsenic fluorescent quantum dot, a preparation method and application thereof, and the molecular formula of the quantum dot is As 4 S 4 @HA QDs, where As 4 S 4 and HA are realgar and hyaluronic acid, respectively. The arsenic fluorescent quantum dots use hyaluronic acid as a stabilizer to form a stable HA hydration film on its surface. The acid is used as a stabilizer, and hydrochloric acid is added to adjust the pH to form arsenic fluorescent quantum dots with stable morphology and structure. The quantum dot of the invention is simple in preparation method and stable in structure, can track intracellular delivery in real time by means of spontaneous strong fluorescence, and specifically recognize tumor cells through hyaluronic acid ligand, realize tumor-targeted delivery, and selectively kill tumor cells. And reduce potential toxic and side effects, with broad application prospects.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials and nanobiology, in particular to an arsenic fluorescent quantum dot and a preparation method and application thereof. Background technique [0002] Realgar (As4S4) is a representative traditional Chinese medicine arsenide, which has a wide anti-cancer spectrum. Its tumor treatment mechanisms mainly include: inducing tumor cell apoptosis, inhibiting tumor proliferation, promoting tumor cell differentiation and anti-tumor angiogenesis. However, there are many problems that need to be solved urgently in the application of realgar in anti-tumor. Realgar is a crystal with high lattice energy, insoluble in water and other common reagents, and its formulation has limitations. It is often administered in large doses orally, but it has poor gastrointestinal absorption and low bioavailability, which reduces the therapeutic effect. The effect of long-term use is potentially toxic, and it is also a w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K47/61A61K33/36A61P35/00B82Y5/00B82Y40/00
CPCA61K49/0019A61K49/0054A61K47/61A61K33/36A61P35/00B82Y5/00B82Y40/00
Inventor 李辉罗红梅周文虎陈双华唐婷
Owner 湖南中医药高等专科学校
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