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Method for rapidly detecting substance allelopathy

A materialized and rapid technology, applied in the fields of testing plants/trees, measuring devices, material inspection products, etc., can solve the problems of time-consuming, manpower and material resources in the measurement test, and achieve the effect of saving manpower, uniform growth, and reducing test costs.

Pending Publication Date: 2021-09-28
HUNAN AGRI BIOTECH RES CENT +1
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  • Application Information

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Problems solved by technology

[0004] Aiming at the technical problems such as time-consuming, manpower, and material resources for the determination of the allelopathic effect of the existing substances, the purpose of the present invention is to provide a method for rapidly detecting the allelopathic effect of the substance, which can save the measurement of the root length and bud length of the recipient plant. Time, a large number of statistical measurements of the allelopathic effect of the substance to be tested in a short period of time

Method used

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  • Method for rapidly detecting substance allelopathy
  • Method for rapidly detecting substance allelopathy
  • Method for rapidly detecting substance allelopathy

Examples

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Embodiment 1

[0029] (1) Germination of recipient plants: sterilize the purchased Qingfengmeiyu lettuce seeds, barnyardgrass seeds and ryegrass seeds collected in the laboratory with 0.3% sodium hypochlorite for 5-10 minutes, and put the sterilized seeds into the mat for two In a 9cm petri dish with one layer of filter paper, add 5ml of clear water to the dish. Lettuce seeds and ryegrass seeds were placed in a light incubator with a temperature of 15°C / 20°C and 12h / 12h; dew white.

[0030] (2) Prepare medium: prepare 1.2% agar aqueous solution. When the temperature of the prepared agar aqueous solution drops to 60°C±2°C, add isovaleric acid, linalool, linoleic acid, dipentene and nonanoic acid (the last 4 substances are dissolved in a small amount of absolute ethanol in advance) to prepare into a 1ml / L agar solution. At the same time, the agar solution without adding allelochemicals was prepared as a control.

[0031] (3) Culture medium transfer: Use a pipette gun to draw 8.5ml of agar ...

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Abstract

The invention belongs to the technical field of bioassay, and particularly relates to a method for rapidly detecting substance allelopathy, which comprises the following operation steps: (1) accelerating germination of a receptor plant, (2) preparing a culture medium, (3) transferring the culture medium, (4) culturing the receptor plant, (5) reading data, and (6) calculating an allelopathy index. The measuring time of root length and bud length of the receptor plant can be saved, and the allelopathy of the chemical substance to be measured can be counted and measured in a large amount in a short time.

Description

technical field [0001] The invention relates to the technical field of bioassay, in particular to a method for rapidly detecting allelopathic effects of substances. Background technique [0002] Allelopathy refers to the beneficial or unfavorable effect of the metabolic secretions of plants or microorganisms on other plants or microorganisms in the environment. The medium of plant allelopathy is a chemical substance, which is called "Allelochemical". Allelochemicals are non-nutritional substances produced in organisms that can affect the growth, health, behavior or group relationship of other plants, mainly secondary metabolites of plants. In recent years, the research on plant allelopathy has been very active. Through this research, it can not only guide the production practice well, realize the virtuous cycle of the agricultural ecosystem, establish reasonable cultivation techniques and farming systems, but also use plant allelopathy Based on the theory, the natural herb...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 柏连阳杨浩娜王立峰邬腊梅周小毛曾浩
Owner HUNAN AGRI BIOTECH RES CENT
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