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Rooting and transplanting method for papaya tissue culture seedlings

A technology of papaya and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as not very ideal rooting quality, poor operability, and poor operability, and achieve saving Seedling time, reduce mutual pollution, and avoid the effect of mutual pollution

Pending Publication Date: 2021-10-01
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is not very operable and cumbersome in the actual production process. At the same time, according to the rooting quality pictures reported in the patent, the rooting quality is not very ideal, and there will still be swollen roots.
[0006] The patent "A Simple, Efficient and High-quality Rooting Method for Papaya Tissue Cultured Seedlings" filed by Zhou Chenping in 2020 mentioned that the incision of the stem end of a single bud was soaked in a rooting induction solution (containing indole butyric acid 1g / L+ Lanthanum chloride 0.1mg / L+phlorizin 10mg / L) in 2min for adventitious root induction to promote root differentiation; this is not very operable in large-scale production

Method used

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  • Rooting and transplanting method for papaya tissue culture seedlings
  • Rooting and transplanting method for papaya tissue culture seedlings
  • Rooting and transplanting method for papaya tissue culture seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for rooting and transplanting papaya tissue culture seedlings, comprising the steps of:

[0029] Step 1: Use papaya field mature lateral buds as explants, sterilize and implant them into the culture medium for primary induction and secondary rejuvenation until adventitious buds are obtained. figure 1 shown.

[0030] Step 2: Cut off the adventitious buds from the base, dip the cross-section of the base with high-temperature sterilized rooting powder, and then transfer to a glass test tube containing 1 / 2MS++20g / L sucrose and vermiculite 1:1 mixed medium After 15-22d, the papaya tissue culture seedlings were obtained as attached figure 2 shown;

[0031] Step 3: Continuing from the previous step, wash the vermiculite at the root of papaya tissue culture seedlings, and remove leafless, rootless and damaged seedlings, such as attached image 3 shown.

[0032] Step 4: Put papaya tissue culture seedlings directly into seedling cups containing perlite and organic m...

Embodiment 2

[0038] The difference between embodiment two and embodiment one is that a kind of papaya tissue culture seedling rooting method uses a kind of culture container 11, as attached Figure 5 And attached Image 6 As shown, the culture container 11 is made of a plastic transparent material resistant to high temperature and high pressure. The culture container 11 is provided with several culture holes 13 arranged in an array. The cross section of the culture holes 13 is square, and the cover plate 12 is provided with a sealing strip (Fig. not shown), the sealing strip is made of rubber material. It also includes a number of hole covers for sealing the culture holes, and the hole covers are all provided with air-permeable filter membranes, and the air-permeable filter membranes are provided with vent holes (not shown in the figure).

[0039] When performing step 2, put 1 / 2MS++20g / L sucrose and vermiculite 1:1 mixed medium into the culture well 13 , and then plant adventitious buds i...

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Abstract

The invention relates to the technical field of papaya tissue culture, and discloses a rooting and transplanting method for papaya tissue culture seedlings. The rooting and transplanting method comprises the following steps of step 1, taking papaya field adult lateral buds as explants, disinfecting and sterilizing, and implanting into a culture medium for primary induction and subculture rejuvenation culture until adventitious buds are obtained; step 2, cutting off the adventitious buds from bases, dipping the cross sections of the bases with rooting powder subjected to high-temperature sterilization, and then transferring the bases into a glass test tube containing a 1 / 2MS + 20g / L sucrose and vermiculite mixed culture medium for culture until papaya tissue culture seedlings are obtained; step 3, washing clean the vermiculite at the roots of the papaya tissue culture seedlings, removing leaves-free, root-free and damaged seedlings, obtaining the papaya tissue culture seedlings, directly putting the papaya tissue culture seedlings into seedling culture cups containing perlite and organic matter, and then controlling temperature and humidity through plastic films and spray irrigation. The rooting and transplanting method is simple, so that papaya tissue culture has operability in large-scale propagation, and the culture efficiency of the papaya tissue culture seedlings is improved.

Description

technical field [0001] The invention relates to the technical field of papaya tissue culture, and specifically discloses a method for rooting and transplanting papaya tissue culture seedlings. Background technique [0002] Many scientific research workers at home and abroad have made some progress and done a lot of useful work in aspects such as papaya breeding and tissue culture. The tissue culture technology has also been reported, but it has not been used on a large scale to replace seedlings in actual production. There are many key restrictive factors, among which the rooting rate of papaya tissue culture seedlings is low, the quality of rooting is not good, the operation process is cumbersome, and the transplanting survival rate is low. [0003] In 2011, the second edition of "Papaya Biotechnology" edited by Zhou Peng and Shen Wentao (Zhou Peng, Shen Wentao. Papaya Biotechnology [M]. Beijing: China Agricultural Press, 2011: 22-42.) was published. In Chapter 2, it is me...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 成镜黄天带戴雪梅顾晓川徐正伟彭素娜李季周权男吴日智
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI