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Microalgae culture medium and culture method

A culture method and culture medium technology, applied in the field of microalgae culture, can solve the problem of negligible yield of microalgae, and achieve the effect of accelerating the growth rate of algae and promoting mitosis

Pending Publication Date: 2021-10-01
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to this method, based on the amount of seedlings of these three aquaculture animals in my country in 2010, 16,000 tons of dry weight of microalgae is required. However, the current production of commercialized microalgae in my country is still less than 10,000 tons. output is negligible

Method used

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  • Microalgae culture medium and culture method
  • Microalgae culture medium and culture method
  • Microalgae culture medium and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Flat coating

[0040] 1.1 Culture medium and preparation

[0041] 1) Nutrient solution mother solution formula (1L)

[0042] Table 1

[0043] Material name molecular formula level Weight (g) sodium nitrate NaNO 3

AR 75 ammonium bicarbonate NH 4 HCO 3

AR 13 Sodium dihydrogen phosphate dihydrate NaH 2 PO 4 2H 2 o

AR 5 Dipotassium hydrogen phosphate trihydrate K 2 HPO 4 ·3H 2 o

AR 3 Potassium dihydrogen phosphate K H 2 PO 4

AR 1.2 Tween 80 Tween-80 AR 0.5ml

[0044] Vitamin Mother Liquid Formula (100ml):

[0045] Table 2

[0046]

[0047] The nutrient solution mother solution was sterilized at 121°C for 20 minutes at high temperature, and after cooling down, 1ml of the vitamin mother solution was added (the mother solution was sterilized by 0.22 membrane filtration in advance).

[0048] 2) Trace element solution mother liquor formula (1L)

[0049] tabl...

Embodiment 2

[0199] Using Chlorella as the algae species, the method and culture medium in Example 1 were compared with common culture media on the market to calculate the growth rate of microalgae. The result is as follows:

[0200] Table 4

[0201]

[0202] Table 5 Algae density results (unit: 10,000 / ml)

[0203] Training time test group Control group 1 Control group 2 Control group 3 0h 2.6 2.3 1.9 1.75 12h 5.8 3.8 4.8 2.55 24h 10.95 7.35 5.5 4.25 36h 15.5 5.9 6.6 5.5 48h 23.8 10.15 5.25 4.6 60h 27.55 8.5 9.2 4.35 72h 36.05 10.1 9.6 3.73

[0204] As a result, it can be seen that the growth rate of the microalgae is more than 3 times that of the control group, and the cell density is higher when the culture medium of the present invention is used for cultivation.

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Abstract

The invention relates to the technical field of microalgae culture, in particular to a microalgae culture medium and a culture method. The culture medium comprises sodium nitrate, ammonium bicarbonate, sodium dihydrogen phosphate dihydrate, monopotassium phosphate trihydrate, monopotassium phosphate, Tween 80, NaCl, trace elements and vitamins. The culture medium provides appropriate nutritional ingredients for the growth of microalgae, can effectively promote the mitosis of algae cells and accelerate the growth speed of algae, and is suitable for large-scale factory culture.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a microalgae culture medium and a cultivation method. Background technique [0002] Microalgae (Microalgae) is a general term for a group of tiny (2-30 micron) lower plants capable of photosynthesis, including eukaryotic and prokaryotic. According to the classic classification method based on the subcellular structure, pigment composition and reproductive mode of microalgae, microalgae are divided into Cyanophyta (cyanobacteria), Chlorophyta, Rhodophyta, Chrysophyta, Diatoms, etc. multiple categories. At present, more than 60,000 species of microalgae have been discovered in the world, more than 6,000 species have been recorded, about several hundred species have been involved in scientific research, and only a few dozen species have been used in production practice. Microalgae are rich in proteins, polysaccharides, fats (polyunsaturated fatty acids), pigments an...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 黄成潭潘军叶蕾黄敏
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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