Method for improving cell proliferation efficiency and anthocyanin yield of vitis davidii

A technology for cell proliferation and thorn grapes, applied in the field of plant tissue culture, can solve the problems of slow growth, instability, low yield of secondary metabolites, etc., and achieve the effects of large light-receiving area, vigorous growth and good stability

Pending Publication Date: 2021-10-08
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in plant cell culture such as slow growth,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1:

[0027] 1. Culture Materials: With a long-term reaction, there is a diaphoside-synthesis ability, and the purple stabbed cell line is cultured.

[0028] 2, tattrain cells cultured in solid medium, select a uniform, consistent, grown-growing stabbed cells that grow vigorously.

[0029] 3. Synchronous proliferation of several cells: 3 days of conventional subsequent subsection of stab and 40 days, shortened to 18 days, will be cultured to 18 days after vaccination (18 days in solid medium The growth of the tissue of the tattoo, the growth is in the initial stage of the growth, and the cell growth is high, and in the ultra-net workbench, the appropriate amount of callus is inoculated into the fresh solid medium, and the stabbed The injuried cells enter the height of growth synchronization in a shorter time.

[0030] 4, performing 3 to 4 generations of synchronous proliferation of several cells, so that the grape cell growth is synchronized, the cell is loose, the p...

Example Embodiment

[0038] Example 2:

[0039] 1. Culture Materials: With a long-term completion of anthocyanin synthesis, a vathlodin synthesis capacity, a purse-shaped suspension cell line is cultured.

[0040] 2, cultured the acupuncture-suspended cells to the mid-exponential growth stage (cultured to 10 days), in the ultra-net workbench, exhaust the triangular bottle so that the cell suspension is evenly dispersed, according to volume ratio 1: 8 (cell suspension Volume: The proportion of fresh liquid medium, 5 ml of tattools suspension with sterilized pipette, transferred into a 150ml triangular bottle equipped with 40 ml of liquid medium, 25 ° C, light intensity 2000 ~ 3000lux, optical cycle 12H light / 12H dark, shaker speed 120 rpm ~ 130 rpm, oscillation culture.

[0041] 3, culturing the tattoo suspension cells for 10 days, until the exponential growth period, in the ultra-net table, according to the ratio of volumetric area ratio 1: 30 ~ 35 (cell suspension volume: solid medium surface area)...

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PUM

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Abstract

The invention provides a method for improving vitis davidii cell proliferation efficiency and anthocyanin yield. The method is realized through the steps of cell line selection, vitis davidii cell suspension culture, cell solid recurrent culture, cell liquid recurrent suspension culture and the like. The vitis davidii callus which is subjected to logarithmic cell synchronous proliferation culture in a solid culture medium is inoculated into a liquid culture medium and is subjected to shaking culture in a shaking table to obtain suspension cells, or the established vitis davidii suspension cells are directly utilized to further culture the vitis davidii suspension cells to obtain vitis davidii suspension cells. A vitis davidii suspension culture cell line with highly dispersed cells, consistent growth state and uniform purple red is established, and recurrent solid culture is performed on the basis of the vitis davidii suspension culture cell line. According to the method, the advantages of solid culture and liquid culture are combined, the genetic stability and vigorous growth capacity of vitis davidii cells can be kept, a large number of vitis davidii cells rich in anthocyanin can be obtained within a short time, and the yield of anthocyanin is high; the anthocyanin can be rapidly produced on a large scale.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for improving the proliferation efficiency and anthocyanin output of grapevine cells. 【Background technique】 [0002] Anthocyanin is a natural food coloring, which is safe and non-toxic. Although anthocyanins widely exist in natural plants, there are limitations in relying on natural plants to extract anthocyanins. At present, anthocyanins are mainly extracted from grape skins in the wine industry. Proanthocyanidins can be used as natural food-derived preservatives. The main sources are pine bark and grape seeds. There is an increasing demand for anthocyanins and proanthocyanidins in industries such as food processing and health products, and the original sources are not enough to meet market demand. Therefore, under the condition of "low-carbon economy", a wider range of materials must be sought to produce grape bioactive substances. Using plant cell ...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/04
Inventor 赖呈纯潘红赖恭梯张静黄贤贵高慧颖王琦
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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