Method for improving cell proliferation efficiency and anthocyanin yield of vitis davidii

Abstract

The invention provides a method for improving vitis davidii cell proliferation efficiency and anthocyanin yield. The method is realized through the steps of cell line selection, vitis davidii cell suspension culture, cell solid recurrent culture, cell liquid recurrent suspension culture and the like. The vitis davidii callus which is subjected to logarithmic cell synchronous proliferation culture in a solid culture medium is inoculated into a liquid culture medium and is subjected to shaking culture in a shaking table to obtain suspension cells, or the established vitis davidii suspension cells are directly utilized to further culture the vitis davidii suspension cells to obtain vitis davidii suspension cells. A vitis davidii suspension culture cell line with highly dispersed cells, consistent growth state and uniform purple red is established, and recurrent solid culture is performed on the basis of the vitis davidii suspension culture cell line. According to the method, the advantages of solid culture and liquid culture are combined, the genetic stability and vigorous growth capacity of vitis davidii cells can be kept, a large number of vitis davidii cells rich in anthocyanin can be obtained within a short time, and the yield of anthocyanin is high; the anthocyanin can be rapidly produced on a large scale.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for improving the proliferation efficiency and anthocyanin output of grapevine cells. 【Background technique】 [0002] Anthocyanin is a natural food coloring, which is safe and non-toxic. Although anthocyanins widely exist in natural plants, there are limitations in relying on natural plants to extract anthocyanins. At present, anthocyanins are mainly extracted from grape skins in the wine industry. Proanthocyanidins can be used as natural food-derived preservatives. The main sources are pine bark and grape seeds. There is an increasing demand for anthocyanins and proanthocyanidins in industries such as food processing and health products, and the original sources are not enough to meet market demand. Therefore, under the condition of "low-carbon economy", a wider range of materials must be sought to produce grape bioactive substances. Using plant cell ...

AI Technical Summary

Problems solved by technology

However, there are still many problems in plant cell culture such as slow growth, low proliferation efficiency, low yield of secondary metabolites, and instability.