Method and kit capable of simultaneously identifying three kinds of fruit flies

Abstract

The invention discloses a kit and a method for rapidly identifying three kinds of fruit flies of bactrocera dorsalis, bactrocera cucurbitae and fruit fly of South Asia. The method comprises the following steps: by taking DNA (Deoxyribose Nucleic Acid) of a to-be-detected fruit fly genome as a template, carrying out PCR (Polymerase Chain Reaction) amplification on an mtCOI gene segment: respectively carrying out enzyme digestion on a PCR product by adopting restriction endonuclease SapI, BsmI and / or Sac I; and judging the types of the fruit flies through gel electrophoresis. According to the invention, the COI gene sequence has sequence difference among different fruit flies, then restriction enzyme is utilized to recognize specific enzyme cutting sites, COI gene segments of three fruit flies can be cut into segments with different sizes, and finally, the three fruit flies can be quickly recognized and identified through agarose gel electrophoresis. The method solves the problem of rapid identification and identification of the three kinds of fruit flies which are seriously harmed and difficult to distinguish on fruits and vegetables, ensures the accuracy of identification results, is more efficient and rapid, greatly saves identification time, and has a very good application prospect.

Description

technical field [0001] The invention belongs to the field of insect molecular biology, and in particular relates to a kit and a method capable of simultaneously identifying three kinds of fruit flies. Background technique [0002] Tephritidae pests belong to the Diptera Tephritidae family Tephritidae. They have a wide variety, covering at least 500 genera and more than 4,500 species. Among them, about 250 species of fruit flies cause direct losses to agricultural production and are a major hazard to fruits and vegetables. Important pests and quarantine pests of economic crops. The female adults of fruit flies like to lay eggs under the skin of melons and fruits. After the eggs hatch inside the fruit, the larvae eat the pulp in the fruit, causing the fruit to rot, fall off, and deteriorate, which has brought huge losses to agricultural production. Precise prevention and control of the above three types of fruit flies, and the complete eradication or blockade of the fruit fli...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that it is difficult to distinguish and identify the larvae of Bactrocera dorsalis and Bactrocera dorsalis, which are seriously harmful to domestic fruits and vegetables, and propose a simple-to-operate method that is not limited by insect state and body. Kits and methods for rapid identification of Bactrocera melons, Bactrocera cerevisiae and Bactrocera dorsalis

Images