Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control

A dynamic speckle, microscopic imaging technology, used in microscopy, fluorescence/phosphorescence, optics, etc., can solve problems such as reducing image contrast and not suitable for thick tissue imaging

Pending Publication Date: 2021-10-19
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

However, structured light microscopy is not suitable for imaging thick tissues be...

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  • Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control
  • Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control
  • Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control

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[0027] The present invention will be further described in detail below in conjunction with examples, so that those skilled in the art can implement it with reference to the description.

[0028] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0029] A dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control, characterized in that: the system is composed of a dynamic speckle illumination wide-field fluorescence microscopic imaging system and a light control system. The system mainly consists of lasers 1, 18; optical fiber beam splitters 2, 5, 6; intensity modulators 11, 12; optical fiber couplers 15; cells to be measured 17; lenses 19, 20, 23, 24; ; Scatterer 22; Apochromat microscope objective lens 25; Dichroic mirror 26; Optical filter 27; Imaging lens 28; CMOS camera 29; Core f...

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Abstract

The invention provides a dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control. The device is characterized in that a light control part of the device is formed by mounting a four-core optical fiber of which the output end face is processed into a specific angle. A laser beam is divided into four beams through a beam splitter, the four beams are coupled into a four-core optical fiber through single-mode optical fibers respectively, a Bessel light field can be formed near the output end face, cells to be detected are stably captured, the output power of fiber cores of the optical fibers can be changed by adjusting a modulator, and the cells are made to rotate around a specific axis. And after the cells rotate to a certain angle and reach a stable state every time, a tomographic image of the cells is acquired by using a dynamic speckle illumination wide-field fluorescence microscopy technology, and a three-dimensional structure image of the cells is finally reconstructed. The constructed system has the advantages of being simple in structure, low in cost, easy to operate and the like, and has wide application prospects in many research fields such as biology, medicine and life science.

Description

(1) Technical field [0001] The present invention relates to a dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber control, which stably captures and precisely controls the rotation angle of living single cells through a four-core optical fiber to realize dynamic speckle wide-field illumination The rapid scanning can be used for the capture and control of living single cells and the imaging of three-dimensional structures with high time resolution and high spatial resolution, belonging to the fields of biological optical tweezers and biological imaging. (2) Background technology [0002] Cells are the basic units that make up the shape and function of organisms, and they themselves are composed of many parts. Therefore, the research on cell structure not only needs to know which parts it is composed of, but also needs to further clarify the composition of each part. Correspondingly, with regard to function, it is not only neces...

Claims

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Application Information

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IPC IPC(8): G01N21/64G02B21/00G02B21/32G02B21/36
CPCG01N21/6458G01N21/6428G01N21/6402G01N21/645G02B21/0032G02B21/0076G02B21/32G02B21/361G01N2021/6439G01N2021/6471G01N2021/6478
Inventor 尹君王少飞于凌尧陈宏宇胡徐锦贾源苑立波
Owner GUILIN UNIV OF ELECTRONIC TECH
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