Dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control

Abstract

The invention provides a dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber light control. The device is characterized in that a light control part of the device is formed by mounting a four-core optical fiber of which the output end face is processed into a specific angle. A laser beam is divided into four beams through a beam splitter, the four beams are coupled into a four-core optical fiber through single-mode optical fibers respectively, a Bessel light field can be formed near the output end face, cells to be detected are stably captured, the output power of fiber cores of the optical fibers can be changed by adjusting a modulator, and the cells are made to rotate around a specific axis. And after the cells rotate to a certain angle and reach a stable state every time, a tomographic image of the cells is acquired by using a dynamic speckle illumination wide-field fluorescence microscopy technology, and a three-dimensional structure image of the cells is finally reconstructed. The constructed system has the advantages of being simple in structure, low in cost, easy to operate and the like, and has wide application prospects in many research fields such as biology, medicine and life science.

Description

(1) Technical field [0001] The present invention relates to a dynamic speckle fluorescence microscopic imaging method and system based on four-core optical fiber control, which stably captures and precisely controls the rotation angle of living single cells through a four-core optical fiber to realize dynamic speckle wide-field illumination The rapid scanning can be used for the capture and control of living single cells and the imaging of three-dimensional structures with high time resolution and high spatial resolution, belonging to the fields of biological optical tweezers and biological imaging. (2) Background technology [0002] Cells are the basic units that make up the shape and function of organisms, and they themselves are composed of many parts. Therefore, the research on cell structure not only needs to know which parts it is composed of, but also needs to further clarify the composition of each part. Correspondingly, with regard to function, it is not only neces...

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Problems solved by technology

However, structured light microscopy is not suitable for imaging thick tissues because the sample's scattering of structured light will reduce the contrast of the image.

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