Multilayered cationic liposome for enhancing skin absorption and preparation method therefor
A cationic liposome and cationic lipid technology, applied in skin care preparations, cosmetic preparations, coatings, etc., can solve problems such as skin barrier function obstruction, improve stability and skin safety, and enhance absorption Effect
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Embodiment 1
[0044] Embodiment 1, the preparation of cationic liposome
[0045] Preparation of cationic liposomes by thin film hydration method. Put Egg pc (L-α-phosphatidylcholine) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP: 1,2-dioleoyl-3-trimethylammonium- propane), ceramide, and cholesterol, were dissolved in 20 mL of chloroform-methanol (4:1) until completely dissolved, and then the solvent was completely removed by a rotary evaporator, thereby forming a thin film on the wall of the flask. After the formed lipid layer was vacuum-dried for 12 hours to completely remove the residual solvent, 10 mL of purified water was added for hydration, and then homogenized for 5 minutes using a probe sonicator. The liposome solution thus obtained was sieved with a 0.45 μm filter (Minisart CA 26 mm) and used for experiments.
Embodiment 2
[0057] The formulas of embodiment 2 and comparative examples 3 to 5 are shown in table 2 below.
[0058] 【Table 2】
[0059]
experiment example 1
[0060] Experimental Example 1, Physical Property Evaluation of Liposome Particles
[0061] 1.1 Confirm particle size and zeta potential
[0062] To measure the particle size and zeta potential of ordinary liposomes and cationic liposomes, a dynamic light scattering device (DLS, SZ-100, HORIBA) was used under neutral conditions (pH 7). The particle size and zeta potential were measured weekly for 4 weeks after preparation, and the results are shown in Figure 1 to Figure 4a .
[0063] The result is as figure 1 As shown, it was confirmed that the particle diameter of ordinary liposomes was 180 to 200 nm, and that of cationic liposomes was 100 to 120 nm, and as figure 2As shown, the zeta potential of ordinary liposomes is -10 to 0 mV, the zeta potential of cationic liposomes is 20 to 50 mV, and its surface charge is cationic. That is, when the stability of the particle size and zeta potential of the cationic liposome of Example 1 containing cholesterol and ceramide was obser...
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