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Dual-purpose test strip for detecting enrofloxacin and amantadine and preparation method and detection method of dual-purpose test strip

A technology for amantadine and enrofloxacin, which is applied in the field of dual-purpose test strips for detecting enrofloxacin and amantadine and their preparation, can solve the problems of inability to use on-site detection, high operation technical requirements and long operation time. and other problems, to achieve the effect of fast and accurate detection, simple and fast operation, and good stability

Inactive Publication Date: 2021-10-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the published literature at home and abroad, the detection methods of amantadine and enrofloxacin mainly include instrument detection method, fluorescence spectrophotometry and immunoassay method, etc., but these methods may require expensive instruments and equipment, and the detection cost is high, or The operation time is long, the operation technical requirements are high and cannot be used for on-site inspection

Method used

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  • Dual-purpose test strip for detecting enrofloxacin and amantadine and preparation method and detection method of dual-purpose test strip
  • Dual-purpose test strip for detecting enrofloxacin and amantadine and preparation method and detection method of dual-purpose test strip
  • Dual-purpose test strip for detecting enrofloxacin and amantadine and preparation method and detection method of dual-purpose test strip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The present invention also provides a method for preparing a dual-purpose test strip for detecting enrofloxacin and amantadine, which comprises the steps of:

[0051] On the bottom plate, the sample pad, colloidal gold binding pad, nitrocellulose membrane and water-absorbing pad are spliced ​​into a test strip; the nitrocellulose membrane is coated with two detection lines and quality control lines.

[0052] It should be noted that, the above-mentioned bonding can be fixed and bonded with a special fixing tape for test strips, or can be bonded in other ways such as glue strips as required.

[0053] Optionally, place the NC membrane (nitrocellulose membrane) stably, wear finger cots and press the upper part of the two bonding pads (that is, the colloidal gold-adamantane bonding pad and the colloidal gold-enrofloxacin bonding pad) to the lower part of the NC membrane And fix it, press the upper part of the sample pad on the lower part of the binding pad and fix it; the ov...

Embodiment 1

[0103] This embodiment provides a dual-purpose test strip for detecting enrofloxacin and amantadine and a preparation method thereof.

[0104] (1) Prepare the conjugates of colloidal gold and amantadine antibody and the conjugates of colloidal gold and enrofloxacin antibody.

[0105] In this example, a conjugate of colloidal gold and amantadine antibody was prepared first. Antibody preparation: The amantadine antibody to be used was dialyzed once with PB, dialyzed twice with distilled water, and the dialysate was changed every 4 hours. After lyophilization, it was dissolved in ultrapure water to 2 mg / mL.

[0106] Screen the best labeling conditions: prepare five 1.5mL centrifuge tubes, add 1mL colloidal gold and 50μL 0.1M potassium borate solution to each tube. Gently invert to fully mix the solution to prevent violent shaking from destroying the stability of the colloidal gold solution. Add 1 μL, 2 μL, 3 μL, 4 μL, and 5 μL of the above-mentioned 2 mg / mL antibody solution t...

experiment example 1

[0116] In this experimental example, the dual-purpose test strip prepared in Example 1 is evaluated for the specificity of the test strip.

[0117] Standard gradient solutions of enrofloxacin and amantadine are used respectively to determine whether the test strip of the present invention can simultaneously detect enrofloxacin and amantadine without crossover.

[0118] When using 200ng / mL enrofloxacin standard solution alone ( image 3 Middle panel in ), all test strips showed no color on the enrofloxacin T line.

[0119] When using 1000ng / mL amantadine standard solution alone ( image 3 In the left picture), all test strips showed no coloration of the amantadine T line.

[0120] And in the negative control (the negative control is the pretreatment solution, image 3 Right panel in ), all strips showed both T-lines were colored.

[0121]It shows that the simultaneous detection test strip of the present invention can realize specific detection between enrofloxacin and amant...

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Abstract

The invention discloses a dual-purpose test strip for detecting enrofloxacin and amantadine and a preparation method and a detection method of the dual-purpose test strip, and relates to the technical field of molecular detection. Two substances of amantadine and enrofloxacin can be detected at the same time through the two detection lines, no instrument or equipment is needed, operation is easy, convenient and rapid, the result can be read through naked eyes during manual operation,and the efficiency of on-site detection of residual standard exceeding of amantadine and enrofloxacin is greatly improved. The dual-purpose test strip has a wide prospect in rapid immunodetection application of enrofloxacin and amantadine drugs, and meanwhile, the proposal of the dual-purpose test strip also provides strong guarantee for market monitoring. The test strip has the advantages of good specificity and good stability. In addition, the invention further provides a method for detecting enrofloxacin and / or amantadine, and the detection method can meet the requirement for rapid and accurate detection of enrofloxacin and amantadine.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a dual-purpose test strip for detecting enrofloxacin and amantadine, a preparation method and a detection method thereof. Background technique [0002] Enrofloxacin is the third-generation fluoroquinolone broad-spectrum antibacterial drug. Due to the gradual change of the basic molecule, the antibacterial performance of enrofloxacin is greatly improved, the pharmacokinetics is improved, and the possibility of adverse reactions is reduced. Antibacterial drugs are widely used in bacterial and mycoplasma infections. Enrofloxacin can target and inhibit the synthesis of bacterial gyrase and topoisomerase, thereby affecting the replication and growth stages of bacteria. According to national regulations, the maximum residue limit of enrofloxacin is 0.1mg / mL. Intake of large doses of enrofloxacin can cause acute poisoning in animals; long-term low-dose intake can also caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/9446G01N33/587G01N33/558
Inventor 邱静宁香雪贾琪钱永忠
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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