Detection method of sugar anhydride content for controlling quality of dextran raw materials
A technology of dextran and a detection method, which is applied in the field of detection of dextran content, can solve problems such as unseen dextran, and achieves the effects of stable detection effect, good reproducibility and good parallelism
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Embodiment 1
[0026] A detection method for controlling the content of dextran raw material dextran, comprising the following steps:
[0027] A. Preparation of test sample: Accurately weigh 0.1g of dextran 20 raw material, add water to dissolve and dilute to 500mL, take 10mL of this solution and then dilute to 100mL to obtain dextran 20 raw material dilution, which is the test sample;
[0028] B. Measure 3mL of the test sample in a test tube, put it in ice water and cool it down to 0°C; mix sulfuric acid and water with a volume ratio of 19:1, put it in an ice bath and cool it down to 0°C, then add anthrone, Stir to dissolve it completely, and obtain an anthrone sulfuric acid solution with a concentration of 0.2% W / V; add 6 mL of anthrone sulfuric acid solution into the above test tube, mix well, and then immediately immerse in a constant temperature water bath at 100°C for boiling water bath heating for 12 minutes, Take it out, measure the absorbance at 625nm with a spectrophotometer after ...
Embodiment 2
[0034] A. Preparation of test sample: Accurately weigh 0.15g of dextran 20 raw material, add water to dissolve and dilute to 500mL, take 10mL of this solution and then dilute to 100mL to obtain dextran 20 raw material dilution, which is the test sample;
[0035] B. Measure 3mL of the test sample in a test tube, put it in ice water and cool it down to 0°C; mix sulfuric acid and water with a volume ratio of 19:1, put it in an ice bath and cool it down to 0°C, then add anthrone, Stir to dissolve it completely, and obtain an anthrone sulfuric acid solution with a concentration of 0.2% W / V; add 6 mL of anthrone sulfuric acid solution into the above test tube, mix well, and then immediately immerse in a constant temperature water bath at 100°C for boiling water bath heating for 12 minutes, Take it out, measure the absorbance at 625nm with a spectrophotometer after cooling;
[0036] C. Blank control: replace the dextran 20 raw material dilution with water, measure the absorbance acco...
Embodiment 3
[0041] A. Preparation of test sample: Accurately weigh 0.2g of dextran 20 raw material, add water to dissolve and dilute to 500mL, take 10mL of this solution and dilute to 100mL to obtain dextran 20 raw material dilution, which is the test sample;
[0042] B. Measure 3mL of the test sample in a test tube, put it in ice water and cool it down to 0°C; mix sulfuric acid and water with a volume ratio of 19:1, put it in an ice bath and cool it down to 0°C, then add anthrone, Stir to dissolve it completely, and obtain an anthrone sulfuric acid solution with a concentration of 0.2% W / V; add 6 mL of anthrone sulfuric acid solution into the above test tube, mix well, and then immediately immerse in a constant temperature water bath at 100°C for boiling water bath heating for 12 minutes, Take it out, measure the absorbance at 625nm with a spectrophotometer after cooling;
[0043] C. Blank control: replace the dextran 20 raw material dilution with water, measure the absorbance according to...
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