Echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application

Abstract

The invention belongs to the technical field of biotechnology, and particularly discloses an echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application. Theechinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine comprises a CD8 + T cell epitope, a CD4 + T cell epitope and a B cell epitope of an EgTeg protein and an EgFABP1 protein. The invention further discloses a screening method and application of the multi-epitope vaccine, and compared with a vaccine obtained by a traditional antigen epitope screening method, after the EgTeg and EgFABP1 multi-epitope vaccine provided by the invention is inoculated, the wet weight of the echinococcus granulosus cyst in a mouse body infected by echinococcus granulosus protoscolex can be effectively reduced; and the screening time and economic cost are low, the experimental process is simplified, and the echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine is suitable for wide screening, popularization and application of the antigen epitopes.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to a multi-epitope vaccine of Echinococcus granulosus EgTeg and EgFABP1 and its application. Background technique [0002] Echinococcus granulosus (Echinococcus granulosus) belongs to platyhelminth phylum, cestode class, round leaf order, belt family, echinococcus genus in taxonomy, and the larval stage is echinococcus granulosus. The full length of the Eg genome is 151.6Mb, encoding 11325 proteins, including 2231 transport proteins and 1748 secretory proteins. In the genome of Echinococcus granulosus, the GC content accounts for about 42.1%, and compared with other parasitic worms, the GC content in the chromosome of Echinococcus granulosus is the highest. Echinococcus granulosus contains complete glycolytic pathway, pentose phosphate pathway and tricarboxylic acid cycle, but lacks the de novo synthesis of pyrimidine, purine and most amino acids (except alanine, ...

AI Technical Summary

Problems solved by technology

[0003] Traditional epitope screening methods include peptide scanning technology, amino acid site-directed mutagenesis technology, X-ray diffraction or nuclear magnetic resonance technology, mass spectrometry technology, phage display peptide technology, etc. These technologies are expensive and expensive, and the experimental steps are cumbersome, which is not conducive to the detection of antigenic epitopes. Extensive screening and promotion of applications

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