Echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application

A technology of Echinococcus granulosus and multi-epitope, which is applied in the direction of medical preparations containing active ingredients, invertebrate antigenic components, material inspection products, etc., can solve the problem of complicated experimental steps and extensive screening and promotion of unfavorable antigenic epitopes Application, high economic cost and other problems, to achieve the effect of improving immunogenicity

Active Publication Date: 2021-11-02
THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] Traditional epitope screening methods include peptide scanning technology, amino acid site-directed mutagenesis technology, X-ray diffraction or nuclear magnetic resonance technology, mass spectrometry technology, phage d...

Method used

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  • Echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application
  • Echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application
  • Echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application

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Embodiment Construction

[0025] The following is further described in detail through specific implementation methods:

[0026] 1. This example discloses Echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccines and their application in the treatment of Echinococcus granulosus.

[0027] The protein sequence of the multi-epitope vaccine is shown in SEQ ID NO:1. Multi-epitope vaccine including CD8 of EgTeg protein and EgFABP1 protein + T cell epitopes, CD4 + T cell epitopes as well as B cell epitopes. CD4 is preferred in this embodiment + T cell epitopes optimized for CD4 + T cell epitope.

[0028] 2. The embodiment also discloses a screening method for the above multi-epitope vaccine.

[0029] Multi-epitope vaccines specifically go through the following steps:

[0030] S1, obtaining the protein sequences of EgTeg and EgFABP1 proteins;

[0031] Obtain the protein sequences of EgTeg and EgFABP1 proteins from the official website of NCBI (https: / / www.ncbi.nlm.nih.gov / genbank / ) for sc...

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Abstract

The invention belongs to the technical field of biotechnology, and particularly discloses an echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine and application. Theechinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine comprises a CD8 + T cell epitope, a CD4 + T cell epitope and a B cell epitope of an EgTeg protein and an EgFABP1 protein. The invention further discloses a screening method and application of the multi-epitope vaccine, and compared with a vaccine obtained by a traditional antigen epitope screening method, after the EgTeg and EgFABP1 multi-epitope vaccine provided by the invention is inoculated, the wet weight of the echinococcus granulosus cyst in a mouse body infected by echinococcus granulosus protoscolex can be effectively reduced; and the screening time and economic cost are low, the experimental process is simplified, and the echinococcus granulosus EgTeg and EgFABP1 multi-epitope vaccine is suitable for wide screening, popularization and application of the antigen epitopes.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to a multi-epitope vaccine of Echinococcus granulosus EgTeg and EgFABP1 and its application. Background technique [0002] Echinococcus granulosus (Echinococcus granulosus) belongs to platyhelminth phylum, cestode class, round leaf order, belt family, echinococcus genus in taxonomy, and the larval stage is echinococcus granulosus. The full length of the Eg genome is 151.6Mb, encoding 11325 proteins, including 2231 transport proteins and 1748 secretory proteins. In the genome of Echinococcus granulosus, the GC content accounts for about 42.1%, and compared with other parasitic worms, the GC content in the chromosome of Echinococcus granulosus is the highest. Echinococcus granulosus contains complete glycolytic pathway, pentose phosphate pathway and tricarboxylic acid cycle, but lacks the de novo synthesis of pyrimidine, purine and most amino acids (except alanine, ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P33/10G01N33/68
CPCA61K39/0003A61P33/10G01N33/6845Y02A50/30
Inventor 张峰波于明凯丁剑冰李玉娇朱玥洁马海梅庞楠楠安梦婷
Owner THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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