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Dry film dressing for promoting quick healing of diabetic refractory wounds and preparation method of dry film dressing

A technology of diabetes and dry film, which is applied in the direction of bone/connective tissue cells, animal cells, medical science, etc., and can solve the problem of insufficient promotion of diabetic refractory wounds, gel preservation, high requirements for transportation conditions, and chitosan coagulation. Inconvenient storage of glue and other problems, to achieve the effect of maintaining high activity and stability, overcoming the difficulty of clinical use, and accelerating wound healing

Pending Publication Date: 2021-11-02
石家庄市第二医院 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gel as a wound dressing is currently a popular research object at home and abroad, but the gel has high requirements in terms of storage and transportation conditions, and the operation in clinical use is relatively complicated, which has certain limitations
Chitosan gel has the advantages of sterilization, non-toxicity, high biocompatibility and biodegradability, etc., but chitosan gel is inconvenient to store, and its promotion effect on diabetic refractory wounds is not significant enough. A kind of dressing that can significantly promote the healing of diabetic refractory wounds and preserve simple and convenient becomes the technical problem to be solved in the present invention

Method used

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  • Dry film dressing for promoting quick healing of diabetic refractory wounds and preparation method of dry film dressing
  • Dry film dressing for promoting quick healing of diabetic refractory wounds and preparation method of dry film dressing
  • Dry film dressing for promoting quick healing of diabetic refractory wounds and preparation method of dry film dressing

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Embodiment 1

[0028] This embodiment relates to a dry film dressing and its preparation for promoting the rapid healing of diabetic refractory wounds.

Embodiment 11

[0030] Dry film dressings for promoting rapid healing of diabetic refractory wounds were prepared as follows.

[0031] a. Preparation of stem cell secretome

[0032] a1. To obtain adipose-derived mesenchymal stem cells, refer to the following processing methods:

[0033] Tissue processing:

[0034] ①Transfer the adipose tissue to 50mL centrifuge tubes, 40mL per tube, centrifuge at 800g at 4°C for 8min, transfer the centrifuged adipose tissue to a new centrifuge tube, add an equal volume of 0.9% normal saline, and centrifuge at 800g at 4°C 8min; Repeat washing once;

[0035] ②Add 0.1% type I collagenase to the fat precipitate in an equal volume, mix well, seal and place in a shaker at 37°C, digest for 45 minutes, take out the digested fat tissue, mix well, and centrifuge for 10 minutes with a centrifugal force of 800g;

[0036] ③ Discard the upper layer of fat to obtain stromal cells, resuspend the cell pellet with RPMI 1640 medium, and centrifuge at 800g for 10 minutes; rep...

Embodiment 12

[0049] Preparation of dry film dressing to promote rapid healing of diabetic refractory wounds: Mix the prepared stem cell secretome and chitosan base gel evenly at a volume ratio of 1:4, fill them into molds, and place them at 24°C After 44 hours, it was dried to form a film and stored at room temperature for later use.

[0050] The preparation of the secretome of adipose-derived mesenchymal stem cells refers to the preparation steps of the secretome of stem cells in Example 1.1. The preparation of the base gel is to mix 3% chitosan chloride solution, 10% β-GP solution, 4% gelatin solution and 4 The %HEC solution was mixed according to the volume ratio of 8:4:4:3, and the shaker was shaken evenly.

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Abstract

The invention relates to a dry film dressing for promoting quick healing of diabetic refractory wounds, which is mainly prepared by uniformly mixing a stem cell secretion group and chitosan basic gel according to a volume ratio of 1: (2-6), and drying to form a film under the conditions that the temperature is 25-42 DEG C and the humidity is 10-20%. The obtained dry film dressing is not limited by storage and transportation conditions, is convenient for doctors and patients to use, and also remarkably improves the healing effect of the dressing on refractory wounds of diabetes mellitus. Meanwhile, the invention further provides a preparation method of the dry film dressing, the high activity and stability of the dressing are guaranteed through convenient operation, and a foundation is laid for clinical application of the dressing.

Description

technical field [0001] The invention relates to the technical field of film dressings, in particular to a dry film dressing for promoting rapid healing of diabetic refractory wounds and a preparation method thereof. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to insulin secretion defects or insulin action disorders. Sustained hyperglycemia and long-term metabolic disorders can lead to damage to tissues and organs throughout the body and their dysfunction and failure. complication. In the past 30 years, the prevalence of diabetes in China has soared from 0.67% to 11.6%, and diabetes has become an epidemic. Among many complications, the risk of diabetic non-healing wounds is as high as 25%. About 4 million diabetic patients worldwide suffer from non-healing wounds every year. The main causes of high refractory diabetic wounds are high glucose toxicity, nerve damage, metabolites, vascular obstruction and insufficie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/28A61L15/20A61L15/32A61L15/44A61L15/46C12N5/0775
CPCA61L15/40A61L15/28A61L15/20A61L15/32A61L15/44A61L15/46C12N5/0667A61L2300/412A61L2300/236A61L2300/232A61L2300/252A61L2300/216A61L2300/404A61L2430/40A61L2300/602C08L5/08C08L1/284
Inventor 孙静华刘立朋刘赞朝赵增毅胡庆山
Owner 石家庄市第二医院
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