A high-pressure carbon dioxide non-thermal extraction method of high-quality phycocyanin pigment

Abstract

The invention discloses a high-pressure carbon dioxide non-thermal extraction method of high-quality phycocyanin pigment, and relates to the technical field of deep processing of natural products. The invention utilizes the high-pressure, anaerobic, and blasting effects of high-pressure carbon dioxide to break cells, extract phycocyanin, separate and take the supernatant, and obtain high-quality phycocyanin aqueous solution, which can be directly used for subsequent processing. The inventors found that the high-pressure carbon dioxide technology has a certain selectivity for cell wall breaking, and the phycocyanin aqueous solution obtained by this method has a high purity. The method described above can also be used for the extraction of phycocyanin from other algal substances. The advantages of the phycocyanin aqueous solution extraction method are: simple operation, short extraction time, good repeatability, energy saving and environmental protection, good extraction quality, industrial production, and it is the first time that high-pressure carbon dioxide sterilization technology is applied to protein extraction. The extracted high-purity phycocyanin aqueous solution provides a scientific basis for the extraction research and industrial production of high value-added natural pigments.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a high-pressure carbon dioxide non-thermal extraction method of high-quality phycocyanin pigment. Background technique [0002] Food "clean label" (Clean label) pursues the product concept of "natural, healthy and safe", which has become a new trend in the development of the global food industry. The use of natural pigments is an inevitable requirement for future food consumption. "Natural without additives, simple ingredients, and uncomplicated process" is the core content of "clean label" products, while synthetic pigments have potential carcinogenic risks. Synthetic pigments and pollutants such as lead and arsenic introduced in the production process will affect consumers. Therefore, many food manufacturers choose to use natural pigments to replace the previous synthetic pigments to meet consumers' demand for natural food and protect consumers' heal...

AI Technical Summary

Problems solved by technology

my country is a big producer of spirulina. At present, there are nearly 70 spirulina factories in China, with a total breeding area of ​​about 7.5 million square meters and an annual output of more than 9,000 tons, accounting for more than 60% of the international market. However, most of the spirulina development in my country is based on raw materials , algae tablets or capsules are exported, and there are few deep-processed products, which limits the development of my country's spirulina industry to a certain extent

[0005] At present, the separation and purification of C-PC is difficult, the extraction process is complicated, and it is easily denatured by light, temperature, microorganisms, etc., which greatly limits the development of its industrial production.

At present, the methods for extracting C-PC pigment from Spirulina include repeated freeze-thaw method, self-heating swelling method, ultrasonic crushing method, enzymatic dissolution method, multifunctional silica gel method, etc. ~8h, time-consuming and energy-consuming, the yield is too low, not suitable for industrial production; self-heating swelling method generally takes 10-20 days, and the wall breaking efficiency is low, and the solution causes a large number of microorganisms to multiply and corrupt; ultrasonic method needs to be used at a specific low concentration It is extracted from the algal cell solution, but it is difficult to realize the propagation of ultrasonic waves in the high-concentration algal cell solution. In addition, the low-concentration algal cell solution also brings about the problem of difficult drying; the preparation of multifunctional silica gel materials is relatively complicated and the process is cumbersome. , the crude extraction process takes 4 hours, which is time-consuming and energy-consuming; the purity of the phycocyanin aqueous solution obtained by the low-salt flocculation method is low, with a purity of 0.4-2.0, and products with a purity below 0.7 do not meet food-grade requirements

[0006] In summary, the current extraction method of phycocyanin is still in the stage of laboratory research, and there is no better process method suitable for industrial production