Enterobacter hormaechei and application thereof in production of vanillin

A technology of Enterobacter halleri and vanillin, which is applied in the field of Enterobacter halleri and its application in the production of vanillin, can solve the problems of poor universality of conditions, long fermentation cycle, and intolerance to high concentrations, etc., and achieve Increase yield, improve production and economic benefits, and solve the effect of long fermentation cycle

Active Publication Date: 2021-11-02
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies, the purpose of the present invention is to provide a kind of Enterobacter hallii and its application in the production of vanillin, which can effectively solve the problem of modern microorganisms using trans-ferulic acid as a substrate to ferment and produce vanillin. Defects such as long fermentation period, poor condition universality, low strain conversion rate and intolerance to high concentration ferulic acid

Method used

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  • Enterobacter hormaechei and application thereof in production of vanillin
  • Enterobacter hormaechei and application thereof in production of vanillin
  • Enterobacter hormaechei and application thereof in production of vanillin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, isolation, screening and identification of strains

[0030] 1. Sample processing

[0031] Weigh 10g each of distiller's grains and Daqu, put them in a 250mL beaker, add 90mL of sterile water, shake well, soak for 30min, and then filter to obtain distiller's grains filtrate and Daqu filtrate, numbered distiller's grains sample liquid (JZ-1~6), Daqu sample liquid ( DH-1~6) for later use; measure 10mL of yellow water into 90mL of sterile water to obtain a yellow water sample solution, numbered yellow water sample solution (HS-1~6) for later use.

[0032] 2. Isolation and purification of bacterial strains

[0033] Use a pipette to take 5 mL of the above three sample solutions and inoculate them in sterilized and cooled LB liquid medium (50 mL) in a conical flask for 48 h on a shaker, with a culture speed of 160 r / min and a culture temperature of 37 °C; repeat Operate this step and carry out enrichment culture again under the same conditions; after enrichment...

Embodiment 2

[0070] Embodiment 2, object strain characteristic

[0071] 1. Growth characteristics of Enterobacter hallii strains

[0072] Depend on Figure 18 It can be seen that when the fermentation culture reaches 0-4h, the curve has almost no trend, indicating that Enterobacter hallii is in a stagnant phase; after the fermentation broth has been cultivated for 4 hours, the growth curve suddenly increases, indicating that Enterobacter hallii enters the logarithmic phase of growth; After culturing for 12 hours, the curve tends to be stable again, and Enterobacter hallii enters the stationary phase at this time.

[0073] 2. Fermentation of Enterobacter hallii strain

[0074] Depend on Figure 19 It can be seen that the fermentation curve of Enterobacter hallii reached the highest peak at 48 hours, indicating that the optimum fermentation time of Enterobacter hallii was 48 hours.

[0075] 3. Morphological characteristics of Enterobacter hallii strains

[0076] The colonies are large, ...

Embodiment 3

[0077] Embodiment 3, bacterial strain fermentation single factor experiment research

[0078] 1. Effect of substrate concentration on the production of vanillin by Enterobacter hallii:

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Abstract

The invention discloses enterobacter hormaechei and application thereof in production of vanillin, and belongs to the technical field of biology, the enterobacter hormaechei is named as DQ and preserved in the China General Microbiological Culture Collection Center on July 26, 2021, and the preservation number is CGMCC No.22958. The enterobacter hormaechei (DQ) can effectively utilize trans-ferulic acid to produce vanillin, the yield and the utilization efficiency of vanillin can be greatly improved, and the strain has good ferulic acid and vanillin tolerance, so that the production and economic benefits are improved, the production in a special environment is met, the defects that the fermentation period is long, the condition universality is poor, the strain conversion rate is low, high-concentration ferulic acid is not resisted and the like when the vanillin is produced by fermentation of modern microorganisms by taking trans-ferulic acid as a substrate are effectively overcome.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an Enterobacter hallii and its application in the production of vanillin. Background technique [0002] Vanillin is the third largest edible spice in the world. With the improvement of people's health awareness, the consumption of natural vanillin is increasing year by year. Modern production methods of vanillin mainly include: chemical synthesis, plant extraction and microbial fermentation. Among them, the glyoxylic acid method is the mainstream method for chemical synthesis, but it has the characteristics of complex products, large process pollution, and low product purity. The plant extraction method is to directly extract vanillin from vanilla plants by extraction and extraction, but at the same time, the planting area of ​​vanilla is limited, the yield is greatly affected by the climate, the planting and processing are cumbersome, and the operation is complicated. As a result,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/24C12R1/01
CPCC12N1/20C12P7/24Y02A50/30
Inventor 杨丽娟刘波叶强张耀张献
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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