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Construction method and application of insulin resistance cell model and combined induction solution of insulin resistance cell model

A technology of insulin resistance and cell model, which is applied in the construction of insulin resistance cell model and the field of combined induction solution of insulin resistance cell model, which can solve the problem of not being able to simulate the pathogenesis of insulin resistance well, and achieve the effect of high sensitivity

Pending Publication Date: 2021-11-05
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Description
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  • Application Information

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Problems solved by technology

[0003] The purpose of the embodiment of this application is to provide a method for constructing an insulin resistance cell model and its application, as well as a joint induction solution for an insulin resistance cell model, which aims to improve the existing insulin resistance cell model that cannot simulate obese people well The problem with the pathogenesis of insulin resistance in vivo

Method used

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  • Construction method and application of insulin resistance cell model and combined induction solution of insulin resistance cell model
  • Construction method and application of insulin resistance cell model and combined induction solution of insulin resistance cell model
  • Construction method and application of insulin resistance cell model and combined induction solution of insulin resistance cell model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This example provides a method for constructing an insulin-resistant cell model and a combined induction solution for the insulin-resistant cell model.

[0065] Culture of 3T3-L1 cells:

[0066] 3T3-L1 cells were cultured in high-glucose DMEM (glucose concentration: 4.5 g / L) medium containing 10% calf serum until the cells were confluent for 48 hours; after adding the first differentiation medium and culturing for 48 hours Then add the second differentiation medium and culture for 48 hours, replace the high-glucose DMEM medium containing 10% fetal bovine serum by volume fraction and continue to culture for 48 hours to obtain differentiated 3T3-L1 cells.

[0067] Wherein, the first differentiation liquid comprises the IBMX that the molar concentration is 0.5mM, the high glucose DMEM medium that the molar concentration is 1 μ M dexamethasone, the mass concentration is 10 μ g / mL insulin and the fetal calf serum of 10% by volume fraction; The second differentiation The sol...

Embodiment 2-4 and comparative example 1-14

[0071] Examples 2-4 and Comparative Examples 1-14 respectively provide a method for constructing an insulin-resistant cell model and a combined induction solution for an insulin-resistant cell model. Please refer to Example 1, the incubation time of Examples 2-4 and Comparative Examples 1-14 and Example 1, the concentrations of fructose, glucose, TNF-α and palmitic acid in the combined induction solution are different, see Table 1 for details.

[0072] Table 1

[0073]

[0074]

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Abstract

The invention relates to the technical field of cell model construction, in particular to a construction method and application of an insulin resistance cell model and a combined induction solution of the insulin resistance cell model. The construction method of the insulin resistance cell model comprises the following steps that cells are incubated in the combined induction solution; the combined induction solution is prepared from sugar with the molar concentration of 20-30 mM, proinflammatory cytokines with the mass concentration of 2-8 ng / mL and palmitic acid with the molar concentration of 0.1-0.6 mM, and the sugar with the molar concentration of 20-30mM is fructose with the molar concentration of 5-15mM and the balance of glucose. The model obtained by the construction method can show glucose absorption reduction, insulin signal channel damage, gluconeogenesis increase and glycogen synthesis reduction and can be accompanied by endoplasmic reticulum stress, oxidative stress, inflammatory response and mitochondrial dysfunction, and the pathogenesis of insulin resistance of obese people under high fructose and high fat diet can be simulated more truly.

Description

technical field [0001] The present application relates to the technical field of cell model construction, in particular, to a method for constructing an insulin-resistant cell model and its application, and a combined induction solution for the insulin-resistant cell model. Background technique [0002] A Western diet characterized by high sugar and high fat is an important cause of obesity. The pathogenesis of obesity-related insulin resistance is very complex. At present, the establishment of insulin resistance cell models in vitro is basically induced by a single factor, and there is a lack of insulin resistance cell models with multiple inducing factors; it cannot well simulate the pathogenesis of insulin resistance in obese people. Contents of the invention [0003] The purpose of the embodiment of this application is to provide a method for constructing an insulin resistance cell model and its application, as well as a joint induction solution for an insulin resistan...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/077C12Q1/02
CPCC12N5/0653C12N5/067C12N5/0658G01N33/5067G01N33/5061G01N33/5044C12N2500/34C12N2500/36C12N2501/25C12N2501/2306C12N2501/2301C12N2501/2318C12N2501/39C12N2501/33C12N2500/40C12N2503/02G01N2500/10
Inventor 郭孝萱
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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