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Application of micro-nano MgH2 compound particles in inhibiting leishmania infection and treating leishmania disease

A Leishmania and compound technology, applied in the field of medicine, can solve the problems of no animal body treatment effect, high pH value dependence of antibacterial activity, difficult separation, etc., achieves good insecticidal inhibitory effect, shortens healing time, inhibits effect of proliferation

Active Publication Date: 2021-11-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, maleimide compounds are single in type, difficult to separate, and some of them are highly toxic, and their antibacterial activity is highly dependent on the pH value. They are passive antibacterial materials that rely on acidic environments (reference: Shen Zhenzhong, New Preparation of Antibacterial Agents Containing Maleimide Structure and Research on Antifungal Activity, Master Thesis of Zhejiang University of Technology, May 2012, P.16)
At the same time, the patent literature only gives in vitro cell experiments and predictions of foreground effects, and does not give examples of actual therapeutic effects in animals.

Method used

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  • Application of micro-nano MgH2 compound particles in inhibiting leishmania infection and treating leishmania disease
  • Application of micro-nano MgH2 compound particles in inhibiting leishmania infection and treating leishmania disease
  • Application of micro-nano MgH2 compound particles in inhibiting leishmania infection and treating leishmania disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] MgH with different particle sizes 2 Compound particles undergo continuous monitoring of hydrogen gas evolution, magnesium ion concentration and pH. The specific method is as follows:

[0038] Three kinds of MgH with particle diameters of 1nm-100nm, 100nm-1000nm, 1um-10um 2 The particles were dropped into the cell culture dish with a final concentration of 1 mg / ml, cultured for 48 hours, and the hydrogen release, magnesium ion concentration and pH value were continuously monitored. The result is as figure 1 shown. From this result, it can be seen that the three different diameters of MgH 2 The particles can generate high concentration of hydrogen at the initial stage, and continue to decrease with time; while the concentration of magnesium ions continues to increase, and the pH value continues to increase and gradually stabilizes at about 8.5.

Embodiment 2

[0040] Micronano MgH 2 The compound particles are co-cultured with macrophages infected by Leishmania (visceral Leishmania donovani), and the specific method is as follows:

[0041] 1) First determine MgH 2 IC50 of compound particles on macrophages (half inhibitory concentration, that is, the concentration at which cell activity is inhibited by half): We co-cultured magnesium hydride particles (100-1000nm) and macrophages derived from THP-1 for 72 hours, and then used CCK -8 detection of cell toxicity ( figure 2 ), found that the IC50 of different culture time points is different:

[0042] 24H:0.261mg / mL

[0043] 48H:0.290mg / mL

[0044] 72H:0.306mg / mL

[0045] 2) Detection of micro-nano MgH 2 Effect of compound particles on THP-1-derived macrophages infected with Leishmania viscera (Leishmania donovani):

[0046] First, determine the optimal number of infections and infection time, by setting different proliferating stage protozoa (5 days or 6 days), infection ratio (5...

Embodiment 3

[0054] Embodiment 3 animal experiment verification

[0055] 1) Biological safety verification: C57BL / 6 mice were selected, and micro-nano MgH with different concentrations (0, 60, 120, 250 and 500mg / kg) were intragastrically administered every day 2 Compound particles (100nm-1000nm), observe various physiological indicators on the eighth day. Such as Figure 5 As shown, the survival rate of the mice was 100%, and the body weight, spleen weight and liver and kidney indexes were all normal.

[0056] 2) Micro-nano MgH 2 Compound Granules Therapeutic Studies:

[0057] 2.1 First, MgH with a particle diameter of 100nm-1000nm 2 After the compound was dissolved in phosphate buffer, it was prepared into 5 concentration groups (1mg / 100ul, 3mg / 100ul, 5mg / 100ul, 10mg / 100ul and 15mg / 100ul).

[0058] 2.2 Then establish a mouse model of mucosal skin type Leishmania (Leishmaniabraziliensis) infection, the specific method is:

[0059] Take several hamsters (Mesocrvcetus Guratus), shave t...

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Abstract

The invention discloses an application of micro-nano MgH2 compound particles in inhibiting leishmania infection and treating leishmania disease. The micro-nano MgH2 compound particles can be used for preparing a pharmaceutical composition for inhibiting leishmania, or preparing a pharmaceutical composition for treating skin or mucous ulcer caused by leishmania infection or viscera damage caused by leishmania infection. It is found for the first time that the micro-nano MgH2 particles can significantly reduce the number of leishmania in macrophages, inhibit proliferation of the leishmania and have a very good insecticidal inhibition effect. The micro-nano MgH2 particles disclosed by the invention can be used for rapidly and effectively curing skin and / or mucosa ulcer caused by the leishmania and functional damage of visceral organs (mainly liver and spleen organs) caused by the leishmania, are good in biological safety and has a huge clinical application value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of a micronano MgH2 compound particle in inhibiting Leishmania infection and treating Leishmaniasis; in particular, it relates to a micronano MgH2 compound particle. 2 Application of compound granules in inhibiting mucocutaneous Leishmania and treating skin and / or mucous membrane ulcers caused by Leishmania infection, as well as functional impairment of internal organs (spleen, liver, bone marrow, etc.) caused by visceral Leishmaniasis damage. Background technique [0002] Leishmaniasis is a disease prevalent in tropical regions. The disease is primarily an intracellular parasitic (Leishmania) disease caused by transmission by sand flies or sandflies. Leishmaniasis is widely distributed around the world, mainly in tropical and subtropical regions, and 98 countries are affected by it. More than 15 million people in Asia, Africa, Southern Europe, and C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/06A61P33/02
CPCA61K33/06A61P33/02Y02A50/30A61K33/00A61P39/06A61K39/015A61K9/14A61K9/0014A61K9/10A61K47/10B82Y30/00A61K9/51B82Y5/00
Inventor 袁广银莫妮卡·埃切弗里·伦登金亮
Owner SHANGHAI JIAO TONG UNIV
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