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Eugenol-sophorolipid nano-emulsion as well as preparation method and application thereof

A nano-emulsion and sophorolipid technology, applied in the field of antibacterial, can solve the problem of no eugenol-sophorolipid nano-emulsion food-borne pathogens, etc., achieve significant antibacterial effect, simple operation method, and broad market prospects

Active Publication Date: 2021-11-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the application of eugenol-sophorolipid nanoemulsion and its preparation and inhibition of the growth of food-borne pathogenic bacteria

Method used

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  • Eugenol-sophorolipid nano-emulsion as well as preparation method and application thereof
  • Eugenol-sophorolipid nano-emulsion as well as preparation method and application thereof
  • Eugenol-sophorolipid nano-emulsion as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 eugenol-sophorolipid nanoemulsion

[0038] Weigh eugenol and sophorolipid according to the mass ratio of 9:1, dissolve in chloroform, dry the solvent with a nitrogen blower, and dry it in vacuum overnight to completely remove the solvent. 100 μL of distilled water was added drop by drop. After each addition, the mixture was vortexed at 800 rpm for 10 min, and left to balance for 30 min. Control the amount of water added until the proportion of the mixed component of eugenol and sophorolipid is 0.5g / L, the turbidity A600 is 0.2, and the average particle size of the emulsion is 137nm measured by laser nanometer particle size analyzer.

Embodiment 2

[0040] Weigh eugenol and sophorolipid according to the mass ratio of 8:2, dissolve in chloroform, dry the solvent with a nitrogen blower, and dry it in vacuum overnight to completely remove the solvent. 100 μL of distilled water was added drop by drop, and after each addition, the mixture was vortexed at 800 rpm for 10 min, left to balance for 30 min, and set aside. Control the amount of water added until the proportion of the mixed component of eugenol and sophorolipid is 0.5g / L, the turbidity A600 is 0.8, and the average particle size of the emulsion measured by laser nanometer particle size analyzer is 175nm.

Embodiment 3

[0042] Weigh eugenol and sophorolipid according to the mass ratio of 1:9, dissolve in chloroform, dry the solvent with a nitrogen blower, and dry it in vacuum overnight to completely remove the solvent. Add 50 μL of distilled water drop by drop. After each addition, the mixture was vortexed at 800 rpm for 10 min, and left to balance for 30 min. Control the amount of water added until the proportion of the mixed component of eugenol and sophorolipid is 10g / L, the turbidity A600 is 1.0, and the average particle size of the emulsion is 301nm measured by laser nanometer particle size analyzer.

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Abstract

The invention discloses a eugenol-sophorolipid nano-emulsion as well as a preparation method and application thereof. The eugenol-sophorolipid nano-emulsion is prepared from eugenol, sophorolipid and water, and the eugenol-sophorolipid nano-emulsion has an inhibition effect on at least one microorganism of Bacillus cereus, Shigella Castellani or Staphylococcus aureus. The eugenol-sophorolipid nano-emulsion is good in stability, eugenol can be solubilized, sophorolipid and eugenol have a synergistic antibacterial effect, the eugenol-sophorolipid nano-emulsion can be used as an ingredient to be applied to the fields of preservatives, antibacterial coating films, antibacterial packaging films and the like, the preparation method is simple, a cosurfactant is not needed, the cost is low, and the eugenol-sophorolipid nano-emulsion has a great market prospect.

Description

technical field [0001] The invention belongs to the antibacterial technology, and in particular relates to a eugenol-sophorolipid nanoemulsion and a preparation method and application thereof. Background technique [0002] Food safety has always been an important global public health issue, and the health hazards caused by foodborne pathogens have attracted much attention. Common food-borne pathogens include Escherichia coli, Staphyloccocus aureus, Bacillus cereus, Listeria monocytogenes, and Salmonella. The use of antibacterial agents is an effective method to effectively inhibit and kill pathogenic microorganisms in food. With consumers' pursuit of healthy products, green, safe and efficient natural antibacterial agents have become an inevitable trend in the research and application of antibacterial agents. [0003] Clove essential oil extracted from clove buds is a light yellow or colorless oily liquid with a special fragrance smell and is a widely used antibacterial dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/349A23L3/3544
CPCA23L3/349A23L3/3544A23V2002/00A23V2200/10A23V2250/2132Y02A50/30
Inventor 范琳琳王英刘小莉周剑忠王帆张宏志朱勇生
Owner JIANGSU ACAD OF AGRI SCI