A continuous flow enrichment device and method for nitrifying bacteria in a suspended state
A technology of nitrification and enrichment in the whole process, applied in the field of microorganisms, can solve the problems of difficult and rapid enrichment and cultivation of nitrifying bacteria, and achieve the effects of efficient interception and proliferation, improvement of residence time, and improvement of enrichment efficiency.
Active Publication Date: 2022-07-19
ZHEJIANG UNIV
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[0005] The purpose of the present invention is to solve the problem in the prior art that the whole process of nitrifying bacteria is difficult to be quickly enriched and cultivated on a large scale, and to provide a
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[0025] The technical solutions of the present invention will be described in detail below with reference to the accompanying drawings and embodiments.
[0026] like figure 1 As shown in the figure, in a preferred embodiment of the present invention, a continuous flow enrichment device for nitrifying bacteria in a suspended state is provided, and its main structure includes an inlet bucket 1, a continuous flow reactor 2, a secondary sedimentation zone 6 and an outlet. For several parts of the bucket 7, the specific composition and connection form of each main structure will be described in detail below.
[0027] The continuous flow reactor is the main enrichment site for the whole process of nitrifying bacteria. Its main structure includes the reactor tank 2, argon aeration device, air aeration device, stirring device, primary sedimentation zone 5 and built-in water outlet pipe 21. The reactor tank 2 is a hollow cylindrical tank, the inner cavity of which can accommodate the r...
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Abstract
The invention discloses a continuous flow enrichment device and method for nitrifying bacteria in a suspended state throughout the whole process. It belongs to the field of sewage treatment microorganisms. The enrichment device includes main structures such as inlet and outlet water, a continuous flow reactor with a built-in primary precipitation zone, and a secondary precipitation zone. Based on the enrichment device, the granulation of suspended sludge is promoted by adding calcium chloride and sodium bicarbonate to the influent; the sludge age SRT can be controlled to be 30-40 days through two-stage precipitation, and the nitrifying bacteria at low growth rate can be promoted throughout the whole process. Retention; by controlling the dissolved oxygen in the reactor at 0.1-0.3 mg / L, the ammonia nitrogen concentration in the enrichment reactor is kept below 0.01 mM, so that the ammonia oxidizing bacteria are not easy to grow and the nitrifying bacteria gradually become the dominant bacteria in the whole process. Under water conditions, the hydraulic retention time (HRT) was gradually reduced to further promote the growth of nitrifying bacteria throughout the process. After 120 days of enrichment according to the present invention, the relative abundance of nitrifying bacteria in the whole process can reach 28.3%. Therefore, the invention has engineering application value in the field of deep denitrification of sewage with low energy consumption.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a continuous flow and suspended state whole-process nitrifying bacteria enrichment technology and device. Background technique [0002] The traditional nitrification theory holds that the autotrophic nitrification process of microorganisms consists of the ammonia oxidation process mediated by ammonia oxidizing bacteria and ammonia oxidizing archaea, and the nitrite oxidation process mediated by nitrite oxidizing bacteria. At the end of 2015, scientists from the United States, the Netherlands, Austria, etc., through enrichment culture and metagenomic analysis of different environmental samples, discovered the whole process of nitrifying bacteria that can independently complete the whole process of nitrification. At present, only one pure whole-course nitrifying bacteria Nitrospira inopinata and one high-enrichment whole-course nitrifying bacteria enrichment "Candidatus Nitr...
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IPC IPC(8): C02F3/12C02F3/34
CPCC02F3/1215C02F3/1236C02F3/34C02F2203/006C02F2305/06C02F2001/007Y02W10/10
Inventor 逯慧杰许少怡柴文波肖锐吴潇隆
Owner ZHEJIANG UNIV