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Method for extracting alpha-mangostin and gamma-mangostin from mangosteen

A technology of mangostin and mangostin, which is applied in the field of extracting α-mangostin and γ-mangostin from mangosteen, can solve the problems of unfavorable industrial production, insufficient utilization of raw materials, numerous process steps, etc., and achieve low cost, High safety and wide application range

Active Publication Date: 2021-11-30
CHANGSHA LANGLIN BIOLOGY PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many steps in this process, the process is relatively complicated, and toxic and harmful organic solvents are used, which is not friendly to the environment. High-speed countercurrent chromatography is used for separation, which is not conducive to large-scale industrial production, and only gamma mangosteen is obtained, and the raw materials are not fully utilized.

Method used

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  • Method for extracting alpha-mangostin and gamma-mangostin from mangosteen
  • Method for extracting alpha-mangostin and gamma-mangostin from mangosteen
  • Method for extracting alpha-mangostin and gamma-mangostin from mangosteen

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Embodiment 1

[0085] The present embodiment is a kind of extracting method of mangosteen bark, comprising the following steps:

[0086] S1. Grind 500 g of mangosteen bark raw material, heat and reflux extract with 10 times volume fraction of 70% ethanol aqueous solution, extract twice, extraction time 2h / time, to obtain extract.

[0087] S2. Concentrate the extract until there is no alcohol smell, separate the solid and liquid, collect the precipitate, add 5 times the volume fraction of 40% ethanol solution to the precipitate to fully dissolve, filter, and use the filtrate for later use.

[0088] S3, the filtrate was purified using HPD400 macroporous resin, and eluted with 1BV water, 3BV volume fraction of 40% ethanol aqueous solution, and 3BV volume fraction of 70% ethanol aqueous solution, and the elution flow rate was 1BV / h; the collection volume fraction was 70 % ethanol solution in water.

[0089] S4. Add 1‰ diatomaceous earth (mass fraction) to the eluent with a volume fraction of 70...

Embodiment 2

[0094] The present embodiment is a kind of extracting method of mangosteen bark, comprising the following steps:

[0095] S1. Grind 500 g of mangosteen bark raw material, heat and reflux extract twice with 10 times volume fraction of 90% ethanol aqueous solution, extraction time is 1 h / time, separate solid and liquid, and collect extract.

[0096] S2. Concentrate the extract under reduced pressure until there is no alcohol smell, separate the solid from the liquid, collect the precipitate, add 10 times the volume fraction of 50% ethanol solution to the precipitate to fully dissolve, filter, and use the filtrate for later use.

[0097] S3. Purify the filtrate in step S2 with LSA-12 resin, and use 2BV of water, 2BV of 50% ethanol aqueous solution, and 3BV of 80% ethanol aqueous solution for elution, and the elution flow rate is 1.5BV / h. The eluate with a volume fraction of 80% ethanol in water was collected.

[0098] S4. Add 5‰ activated carbon (mass fraction) to the eluent wit...

Embodiment 3

[0103] The present embodiment is a kind of extracting method of mangosteen bark, comprising the following steps:

[0104] S1. Grinding 500 g of mangosteen bark raw material, extracting with 10 times the volume fraction of 80% ethanol aqueous solution, extraction temperature is 90° C., extraction 3 times, extraction time 1 h / time, to obtain extract.

[0105] S2. Concentrate the extract until there is no alcohol smell, separate the solid and liquid, collect the precipitate, add 20 times the volume fraction of 40% ethanol solution to the precipitate to fully dissolve, filter, and use the filtrate for later use.

[0106] S3. Purify the filtrate in step S2 with LX-38 resin, use 3BV of water, 1BV of 40% ethanol aqueous solution, and 4BV of 70% ethanol aqueous solution to elute, and the elution flow rate is 2BV / h, collect The volume fraction is the eluent of 70% ethanol aqueous solution.

[0107] S4. Add 3‰ activated carbon (mass fraction) to the eluent with a volume fraction of 70%...

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Abstract

The invention discloses a method for extracting alpha-mangostin and gamma-mangostin in mangosteen. The method comprises the following steps: S1, crushing a mangosteen skin raw material, and performing extracting with an ethanol water solution; S2, concentrating, separating and redissolving the extracting solution; S3, purifying the solution through macroporous resin, and carrying out gradient elution with an ethanol water solution; S4, collecting a target section and performing decolorizing; S5, adding a complexing agent into the decolored solution, performing concentrating under reduced pressure until no alcohol smell exists, and performing solid-liquid separation to obtain an alpha-mangostin crude product; adjusting the pH value of the clear liquid, and carrying out solid-liquid separation to obtain a gamma-mangostin crude product; and S6, respectively carrying out crystallization treatment on the alpha-mangostin crude product and the gamma-mangostin crude product to obtain the high-purity alpha-mangostin and the high-purity gamma-mangostin. According to the method, high-content alpha-mangostin and gamma-mangostin can be prepared at the same time, industrial production can be achieved, and the process is safe, reliable and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting α-mangostin and γ-mangostin in mangosteen. Background technique [0002] Mangosteen Garcinia mangostana L, also known as mangosteen, mangosteen and phoenix fruit, is an evergreen tree of Garcinia genus Garcinia, and it is a traditional medicine in Southeast Asia. Its medicinal and health benefits come primarily from a group of chemicals known as xanthones and other nutrients such as flavonoids. Mangosteen peel contains more than 40 kinds of xanthones, among which the most important xanthones are α-mangostin (α-mangostin) and γ-mangostin (γ-mangostin). Xanthones have strong antioxidant capacity in the phytonutrient family, and supplementing xanthones clinically and scientifically is considered to have a significant inhibitory effect on the formation of myocardial infarction or cerebral infarction. γ-mangostin has antioxidant, antibacter...

Claims

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Application Information

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IPC IPC(8): C07D311/86
CPCC07D311/86Y02A50/30
Inventor 曹慧璋易宇阳贺玉婷
Owner CHANGSHA LANGLIN BIOLOGY PROD
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