Preparation for promoting hair growth and preparation method thereof
A preparation and growth factor technology, applied in the field of medicine, can solve the problems of inconspicuous effect, slow onset of effect, long time, etc., and achieve the effect of facilitating industrialization, promoting hair regeneration, and lasting effect
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Embodiment 1
[0048] Embodiment 1 A kind of preparation that promotes hair growth and preparation method thereof
[0049] 1. The hospital collects the umbilical cord tissue of a full-term cesarean section human fetus, signs the customer's informed consent before the collection, and transports the tissue to the laboratory in a refrigerated and sterile environment at 4°C. During the transportation, the tissue protection solution is used to protect the biological activity of the umbilical cord tissue and protect the tissue. The solution was prepared by adding 25 μg / mL gentamicin sulfate and 5 μg / mL amphotericin B to normal saline to ensure no bacterial and fungal contamination during transportation.
[0050] 2. In the laboratory, use tissue protection solution to clean the blood on the surface of the umbilical cord tissue, remove the epidermis and vascular tissue, take out the Huatong glue, wash it, and cut it into 1-2mm pieces 3 Volume fragments were inoculated in T75 culture flasks by the ti...
Embodiment 2
[0061] Embodiment 2 A kind of preparation that promotes hair growth and preparation method thereof
[0062] The difference from Example 1 is that the ratio in step 11 is: the umbilical cord mesenchymal stem cell cytokine supernatant with a volume fraction of 31%, the venous blood concentrated growth factor CGF with a volume fraction of 62%, and a volume fraction of 7 % human serum albumin, the final concentration is 7.5 μg / mL water-soluble fullerene derivative.
Embodiment 3
[0063] Embodiment 3 A kind of preparation that promotes hair growth and preparation method thereof
[0064] The difference from Example 1 is that the ratio in step 11 is: the umbilical cord mesenchymal stem cell cytokine supernatant with a volume fraction of 25%, the venous blood concentrated growth factor CGF with a volume fraction of 72%, and the volume fraction of 3%. Human serum albumin, the final concentration is 6.5μg / mL water-soluble fullerene derivative.
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