Low-temperature-resistant straw fermentation inoculant and preparation method thereof
A technology for fermenting bacterial agents and straws, which is applied in the field of straw fermenting bacterial agents and its preparation, can solve the problems of incomplete fermentation of straws, and achieve the effect of low cost and simple operation
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Embodiment 1
[0033] 1. Screening of bacterial strains and determination of their disintegration force on filter paper strips
[0034] Strain screening and cultivation: collect rotten leaves under the forest, rotten straw in the field, rotten cow dung, and natural fermented straw compost as isolated samples, and use the straw fishing separation method to separate filamentous fungi; use the enrichment method to separate bacteria and put nematodes. Through long-term screening and low-temperature domestication and breeding, six strains were obtained, namely NH1 Saccharomycescerevisiae, NH2 lactic acid bacteria, NH3 Bacillus subtilis, NH4 photosynthetic bacteria (Photosynthetic Bacteria), NH5 actinomycete (Actinomycete, NH6 Kangshi wood Mold (Trichodermakoningii). The screened strains were purified and inoculated into respective appropriate media for proliferation and culture.
[0035] The optimal combination of Saccharomyces cerevisiae, lactic acid bacteria, Bacillus subtilis, photosynthetic ...
Embodiment 2
[0046] Embodiment 2. application of fermentation agent
[0047] The mass ratio of straw and livestock manure is 2:1 in the straw and the mixture of straw and livestock and poultry manure, and it is transformed into decomposed organic products through solid fermentation. The fermentation of the technical product formula bacterial agent is mainly done in large piles of more than 50 tons to prevent air-drying and freezing through to prevent fermentation. Fermentation bacteria are added in the proportion of 5 / 1000 of the total mass, the water content of the straw is adjusted to about 65%, and the straw is compacted and fermented. Hot spot fermentation is required for winter fermentation, that is, 100 kg of rice husk powder is fermented first, made into hot spot bags, and placed in the center of the straw fermentation pile to guide fermentation. After about 40-50 days of fermentation, the pile is turned over, and the unfermented straw in the outer layer is turned over to the insid...
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