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Method for improving manganese oxidation function of bacteria through algae

A technology of manganese oxidation and bacteria, applied in the field of improving the function of bacterial manganese oxidation, can solve problems such as diseases, and achieve the effect of simple operation and wide application range

Active Publication Date: 2021-12-07
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Manganese is an abundant metal element in the earth's crust, Mn 2+ It is one of the essential trace elements in animal and plant nutrition and human metabolism, but drinking water with excessive manganese for a long time will cause human physiological diseases

Method used

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  • Method for improving manganese oxidation function of bacteria through algae
  • Method for improving manganese oxidation function of bacteria through algae
  • Method for improving manganese oxidation function of bacteria through algae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The influence of embodiment 1 algae cell on bacterial manganese oxidation function

[0037] Cultivation of algae cells: Microcystis aeruginosa algae cells were cultured in BG-11 medium in an artificial climate incubator. The culture was divided into two steps: day and night. The day conditions were: duration 14h, temperature 29°C, The average light intensity is 400-650lx, and the light is irradiated diagonally by fluorescent tubes; the night conditions are: the duration is 10 hours, the temperature is 26°C, and there is no light.

[0038] Surface raw water: pH 8.2, COD Mn 5.0mg / L, TN 1.0mg / L, TP 0.08mg / L.

[0039] Sterilization conditions for surface raw water: Autoclave at 121°C for 20 minutes.

[0040] Resuspended bacteria solution: activate the manganese oxidizing bacteria QJX-1 in PYG medium for 24 hours, centrifuge the obtained bacterial solution at 8000rmp for 10 minutes, remove the supernatant, and resuspend the obtained bacterial solution with an equal volume...

Embodiment 2

[0049] The influence of embodiment 2 algal density on bacterial manganese oxidation function

[0050] Surface raw water: pH 8.1, COD Mn 4.8mg / L, TN 0.8mg / L, TP 0.1mg / L.

[0051] Sterilization conditions for surface raw water: Autoclave at 121°C for 20 minutes.

[0052] Resuspended bacteria solution: activate the manganese oxidizing bacteria QJX-1 in PYG medium for 24 hours, centrifuge the obtained bacterial solution at 8000rmp for 10 minutes, remove the supernatant, and resuspend the obtained bacterial solution with an equal volume of sterilized surface raw water.

[0053] Control group: Add sterilized surface raw water to the Erlenmeyer flask, and add 5.5mg·L -1 mn 2+ and 5ml of resuspended bacteria, the final total volume is 200mL, and the solution contains about 3×10 7 pcs mL -1 bacterial cells.

[0054] Experimental group 1, experimental group 2, and experimental group 3: add 10 5 pcs / mL, 10 7 pcs / mL, 10 8 cells / mL of Microcystis aeruginosa, and the other conditi...

Embodiment 3

[0057] The influence of embodiment 3 co-cultivation supernatant of algal bacteria on bacterial manganese oxidation

[0058] Resuspended bacteria liquid: Activate the manganese oxidizing bacteria QJX-1 in PYG medium for 24 hours, centrifuge the obtained bacterial liquid at 8000rpm for 10 minutes, remove the supernatant and resuspend with an equal volume of surface raw water for use.

[0059] Surface raw water: pH 8.4, COD Mn 5.3mg / L, TN 0.7mg / L, TP 0.06mg / L.

[0060] Co-cultivation supernatant: Microcystis aeruginosa cells were cultured in BG-11 medium, placed in an artificial climate incubator, and fed to the algae culture system in the stationary phase (containing about 10 8 pcs mL -1 Algae cells) add 10 8 pcs mL -1 The manganese oxidizing bacteria QJX-1 cells were mixed evenly and placed in an artificial climate incubator for co-cultivation. After 6, 12, 24, and 48 hours of co-cultivation, the culture system was first centrifuged at 4000rpm for 5 minutes to remove alga...

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Abstract

The invention belongs to the technical field of water treatment, and particularly relates to a method for improving the manganese oxidation function of bacteria through algae. According to the method, manganese oxidizing bacteria and algae are added into to-be-treated water containing heavy metals and organic matters, or the manganese oxidizing bacteria and supernate obtained by co-culturing the manganese oxidizing bacteria and the algae are added for treatment. The biological manganese oxidation process can be enhanced by the exosome generated when phycomycetes coexist, so that the efficient removal of heavy metals and organic matters is realized. The method is simple and easy to implement, can improve the manganese oxidation efficiency of the manganese oxidizing bacteria, shortens the treatment time, saves the cost, and is easy to popularize and implement.

Description

Technical field: [0001] The invention belongs to the technical field of water treatment, and in particular relates to a method for improving the manganese oxidation function of bacteria through algae. Background technique: [0002] Manganese is an abundant metal element in the earth's crust, Mn 2+ It is one of the essential trace elements in animal and plant nutrition and human metabolism, but drinking water with excessive manganese for a long time will cause human physiological diseases. Manganese pollution in water mainly refers to the pollution of divalent manganese, which is more common in surface water source reservoirs, and the phenomenon of manganese exceeding the standard also exists in the surface water of rivers. At the same time, the manganese content of groundwater in many areas of my country also exceeds the national drinking water standard. [0003] Microbes can catalyze the oxidation of Mn 2+ Biomanganese oxides are generated, and many microorganisms in natu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/32C02F3/34C12R1/01C12R1/40C02F101/30C02F101/20
CPCC12N1/20C02F3/325C02F3/34C02F2101/30C02F2101/206
Inventor 戚菁林泽淼赵济金胡承志曲久辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI