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Preparation method of noradrenaline sensor based on mercaptoacetic acid/vitamin B1

A technology of norepinephrine and thioglycolic acid, applied in the field of biosensors, can solve problems such as no biosensors found, and achieve the effects of good specificity, improved stability and good precision

Pending Publication Date: 2021-12-07
齐鲁医药学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Has not yet been found to be composed of thioglycolic acid and vitamin B 1 Related reports on co-fabricated biosensors

Method used

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  • Preparation method of noradrenaline sensor based on mercaptoacetic acid/vitamin B1
  • Preparation method of noradrenaline sensor based on mercaptoacetic acid/vitamin B1
  • Preparation method of noradrenaline sensor based on mercaptoacetic acid/vitamin B1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of thioglycolic acid-modified gold electrodes

[0045] 1.1 Preparation of potassium ferricyanide solution

[0046] Weigh 0.6585g of potassium ferricyanide and 0.7455g of potassium chloride into a 100mL beaker, add distilled water and stir with a glass rod to dissolve them, move them to a 100mL volumetric flask and shake to make a solution of about 2.0× 10 -2 mol / L potassium ferricyanide solution (containing 0.1mol / L potassium chloride).

[0047] 1.2 Bare gold electrode pretreatment

[0048] The gold electrode is made of Al with an average particle size of 0.05 μm 2 o 3 The powder was polished to the mirror surface on a polishing cloth, ultrasonically cleaned with distilled water for 15 minutes, taken out and dried naturally, then ultrasonically cleaned with absolute ethanol for 10 minutes, taken out and rinsed with distilled water on the surface of the electrode, and cleaned at 2.0×10 -2 The mol / L potassium ferricyanide solution (containing 0.1 mol / L...

Embodiment 2

[0063] 1. Preparation of thioglycolic acid-modified gold electrodes

[0064] 1.1 Preparation of potassium ferricyanide solution

[0065] Weigh 0.6914g of potassium ferricyanide and 0.8201g of potassium chloride and place them in a 100mL beaker, add distilled water and stir with a glass rod to dissolve them, transfer them to a 100mL volumetric flask, and shake them evenly to make about 2.1× 10 -2 mol / L potassium ferricyanide solution (containing 0.11mol / L potassium chloride).

[0066] 1.2 Bare gold electrode pretreatment

[0067] The gold electrode is made of Al with an average particle size of 0.05 μm 2 o 3 The powder was polished to the mirror surface on a polishing cloth, ultrasonically cleaned with distilled water for 15 minutes, taken out and dried naturally, then ultrasonically cleaned with absolute ethanol for 10 minutes, taken out and rinsed with distilled water on the surface of the electrode, and cleaned at 2.1×10 -2 The mol / L potassium ferricyanide solution (con...

Embodiment 3

[0082] 1. Preparation of thioglycolic acid-modified gold electrodes

[0083] 1.1 Preparation of potassium ferricyanide solution

[0084]Weigh 0.6256g of potassium ferricyanide and 0.6710g of potassium chloride into a 100mL beaker, add distilled water and stir with a glass rod to dissolve them, move them to a 100mL volumetric flask and shake to make about 1.9× 10 -2 mol / L potassium ferricyanide solution (containing 0.09mol / L potassium chloride).

[0085] 1.2 Bare gold electrode pretreatment

[0086] The gold electrode is made of Al with an average particle size of 0.05 μm 2 o 3 The powder was polished to the mirror surface on a polishing cloth, ultrasonically cleaned with distilled water for 15 minutes, taken out and dried naturally, then ultrasonically cleaned with absolute ethanol for 10 minutes, taken out and rinsed with distilled water on the surface of the electrode, and cleaned at 1.9×10 -2 The mol / L potassium ferricyanide solution (containing 0.09mol / L potassium chl...

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Abstract

The invention belongs to the technical field of biosensors, and particularly relates to a preparation method of a noradrenaline sensor based on mercaptoacetic acid / vitamin B1. According to the method, a mercaptoacetic acid modified gold electrode is taken as a carrier, and vitamin B1 is loaded on the surface of the mercaptoacetic acid modified gold electrode, so that the mercaptoacetic acid / vitamin B1-based noradrenaline sensor is obtained. The gold electrode is modified by mercaptoacetic acid and vitamin B1 together, so that a bilayer is formed on the gold electrode, and the noradrenaline electrochemical sensor is good in stability, specificity and precision and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a biosensor based on thioglycolic acid / vitamin B 1 Preparation method of norepinephrine sensor. Background technique [0002] Norepinephrine (NA or NE), also known as norepinephrine, is an important neurotransmitter in the human body and a catechol transmitter released by the adrenal cortex. NA is an anti-shock vasoactive drug, and it is one of the active drugs commonly used in septic shock clinically. It can quickly revive patients with septic shock and reduce mortality. However, long-term use of NA can cause adverse reactions such as ischemic necrosis, acute renal failure, and blood pressure drop after drug withdrawal. Therefore, the simple, sensitive and rapid detection of NA concentration is of great significance in clinical and drug quality control. [0003] The current methods for detecting NA concentration include spectrophotometry, high performance liquid chromatogr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/49
CPCG01N27/3277G01N27/49
Inventor 高先娟汲霞杜青青任云凤车琳
Owner 齐鲁医药学院