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Electrochemical biosensor, preparation method thereof and method for detecting novel coronavirus

A biosensor, coronavirus technology, applied in the field of electrochemical analysis and detection, can solve problems such as inconvenient signal readout, and achieve the effects of fast detection, low cost and high repeatability

Pending Publication Date: 2021-12-10
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of inconvenient signal readout in the detection of novel coronavirus existing in the prior art, to provide an electrochemical biosensor and its preparation method and method for detecting novel coronavirus, which can realize detection of novel coronavirus Fast, Highly Sensitive Detection

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  • Electrochemical biosensor, preparation method thereof and method for detecting novel coronavirus
  • Electrochemical biosensor, preparation method thereof and method for detecting novel coronavirus
  • Electrochemical biosensor, preparation method thereof and method for detecting novel coronavirus

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preparation example Construction

[0040] In a second aspect, the present invention provides a method for preparing an electrochemical biosensor, comprising the steps of: drip-coating a modification solution containing polyallylamine hydrochloride, ceria and ferrocene-labeled non-specific single-stranded DNA Drying is carried out on the surface of the screen-printed electrode, so that the surface of the screen-printed electrode forms a functional layer containing polyallylamine hydrochloride, ceria, and ferrocene-labeled non-specific single-stranded DNA.

[0041] According to the present invention, the modification solution contains polyallylamine hydrochloride (PAH) / cerium oxide (CeO 2 ) compound solution and the solution containing Fc-ssDNA are mixed in a certain ratio to obtain. Exemplarily, the concentration of PAH in the composite solution is 2.5-7.5mg / mL, CeO 2 The concentration of Fc-ssDNA in the solution containing Fc-ssDNA is 0.5-1.5mg / mL, the concentration of Fc-ssDNA in the solution containing Fc-ss...

Embodiment 1

[0079] (1) Will contain polyallylamine hydrochloride (PAH) and cerium oxide (CeO 2 ) compound solution and Fc-ssDNA concentration of 2μM solution mixed by volume ratio 1:1 to obtain a modification solution, wherein, CeO in the compound solution 2 The concentration of Fc-ssDNA is 1mg / mL, the concentration of PAH is 1mg / mL, 3mg / mL, 5mg / mL, 7mg / mL, 8mg / mL respectively, and the nucleotide sequence of Fc-ssDNA is shown in SEQ ID No.1;

[0080] (2) drip-coat the modification solution on the surface of the screen-printed electrode, and wait to dry overnight at room temperature to prepare an electrochemical biosensor;

[0081] (3) Mix 1 μL of the test sample containing the test virus (2019-nCoV-E) (the concentration of the test virus is 10 copies / μL) with 25 μL of buffer v, 2 μL of primer R solution with a concentration of 2 μM, and 2 μL of a concentration of 2 μM Primer F solution, 2.5 μL of Mg 2+ Magnesium acetate solution with a concentration of 15mmol / L was mixed with 17.5 μL of...

Embodiment 2

[0086] (1) Will contain polyallylamine hydrochloride (PAH) and cerium oxide (CeO 2 ) and a solution with a Fc-ssDNA concentration of 2 μM were mixed at a volume ratio of 1:1 to obtain a modified solution, wherein the concentration of PAH in the complex solution was 5 mg / mL, CeO 2 The concentrations are 0.5mg / mL, 1mg / mL, 2mg / mL, 3mg / mL, 4mg / mL respectively, and the nucleotide sequence of Fc-ssDNA is shown in SEQID No.1;

[0087] (2) drip-coat the modification solution on the surface of the screen-printed electrode, and wait to dry overnight at room temperature to prepare an electrochemical biosensor;

[0088] (3) Mix 1 μL of the test sample containing the test virus (2019-nCoV-E) (the concentration of the test virus is 10 copies / μL) with 25 μL of buffer v, 2 μL of primer R solution with a concentration of 2 μM, and 2 μL of a concentration of 2 μM Primer F solution, 2.5 μL of Mg 2+ Magnesium acetate solution with a concentration of 15mmol / L was mixed with 17.5 μL of purified w...

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Abstract

The invention relates to the field of electrochemical analysis and detection, and discloses an electrochemical biosensor, a preparation method thereof and a method for detecting novel coronavirus. The electrochemical biosensor provided by the invention comprises a screen-printed electrode and a functional layer modified on the surface of the screen-printed electrode, wherein the functional layer contains polyallylamine hydrochloride, cerium dioxide and ferrocene-labeled non-specific single-stranded DNA. The preparation method of the electrochemical biosensor comprises the following steps of dispensing a modification solution containing polyallylamine hydrochloride, cerium dioxide and ferrocene-labeled non-specific single-stranded DNA on the surface of a screen-printed electrode, and drying to form a functional layer on the surface of the screen-printed electrode. The electrochemical biosensor provided by the invention can realize rapid and high-sensitivity detection of novel coronavirus.

Description

technical field [0001] The invention relates to the field of electrochemical analysis and detection, in particular, to an electrochemical biosensor, a preparation method thereof, and a method for detecting novel coronavirus. Background technique [0002] In order to control the source of infection and help patients prevent their condition from getting worse, it is very important to provide a reliable, efficient, fast and accurate method for identifying the RNA of the new coronavirus. Viral nucleic acid can be used for early diagnosis and has been widely used clinically. The current reference method for the detection of new coronavirus is specific and quantitative real-time polymerase chain reaction (RT-qPCR) detection from nasal or throat swabs, sputum or bronchoalveolar instillation. Wash specimen samples. However, RT-qPCR is costly, time-consuming, and requires extensive laboratory infrastructure and training, hindering its current application in resource-constrained sett...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3275Y02A50/30
Inventor 黄和王鑫杰吴成媛吴丽娜
Owner NANJING NORMAL UNIVERSITY
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