Transformation method for improving antigenicity of influenza A virus H1 trimer protein

Abstract

The invention belongs to the technical field of biology, and particularly relates to introduction of a disulfide bond to modify an influenza A virus hemagglutinin H1 protein structure so as to promote an H1 extracellular domain to form a stable trimer state and enhance the antigenicity and specific immune protection effect of the H1 extracellular domain. The method comprises the following steps of replacing a signal peptide sequence of an H1 extracellular domain, adding a tag, and inserting the signal peptide sequence into a pFastBac1 vector to obtain a recombinant plasmid; according to protein structure information and auxiliary software analysis, introducing cysteine mutation into the H1 extracellular domain gene sequence of the recombinant plasmid to obtain various H1 mutant plasmids; expressing a plurality of recombinant H1 proteins by using recombinant baculovirus infection suspension culture insect cells; and obtaining an H1 mutant by screening through a molecular biology experimental method, wherein a more stable trimer state and better antigenicity can be formed. Mouse immune experiments prove that the optimized H1 mutant provides a stronger in-vivo specific immune protection effect.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a transformation method for improving the antigenicity of H1 trimeric protein of influenza A virus. Background technique [0002] Influenza is a human respiratory infectious disease mainly caused by influenza A and influenza B viruses. There are usually seasonal epidemics every year. According to the World Health Organization (WHO), influenza causes about 1 billion infections every year, including 3-5 million severe cases and 300,000-500,000 deaths. Getting vaccinated remains the most effective way to prevent the flu. [0003] Due to the continuous antigenic drift of influenza virus, multivalent seasonal influenza vaccines need to update the vaccine strains every year according to the predicted epidemic strains, but the prediction of the epidemic strains in the next year may not be very accurate, resulting in vaccine strains and current epidemic strains....

AI Technical Summary

Problems solved by technology

However, exogenous trimer motifs are often highly immunogenic and tend to induce strong off-target immune responses. Therefore, the introduction of exogenous trimer motifs has the risk of reducing the specific immune protection of the target antigen

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