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Preparation method and application of silk protein nanofiber

A nanofiber and silk protein technology, applied in the field of preparation of silk protein nanofibers, can solve the problems of low yield of nanofibers, unstable nanofiber structure, inconvenient construction of nanofibers, etc., and achieve simple operation and good biocompatibility And mechanical properties, the effect of broadening the application road

Pending Publication Date: 2021-12-21
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have certain limitations, and there are still defects such as the entanglement between nanofibers, which is not convenient for the construction of materials, the structure of nanofibers is unstable, and the yield of nanofibers is low.

Method used

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  • Preparation method and application of silk protein nanofiber
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  • Preparation method and application of silk protein nanofiber

Examples

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Effect test

Embodiment 1

[0039] The preparation method of silk protein nanofiber dispersion liquid comprises the following steps:

[0040] (1) The source and degumming of silk protein: Mulberry silk is produced in Zhejiang, China. The degumming method of silk fibroin: Mulberry silk is cut into 1cm long, in 0.5% (w / w) NaHCO 3 Boil in the solution for 30min, wash with distilled water to remove NaHCO 3 And sericin, repeat the above steps once, and the degummed silk fibroin is dried at room temperature for later use.

[0041] (2) configure 1wt% sodium hydroxide solution and stir evenly;

[0042] (3) Add degummed mulberry silk, and immerse in sodium hydroxide solution with a solid-to-liquid ratio of 1:10 g / mL to obtain a mixed solution;

[0043] (4) Put the mixed solution in an ice-water bath at -80°C to freeze and then thaw it, accompanied by mechanical stirring, repeated several times, and obtained a solid suspension after 20 hours;

[0044] (5) washing the water-insoluble matter in the solid suspensi...

Embodiment 2

[0048] The preparation method of silk protein nanofiber dispersion liquid comprises the following steps:

[0049] (1) The source and degumming of silk protein: Tussah silk is produced in Zhejiang, China. The degumming method of tussah silk fibroin is: cut tussah silk into 1cm long, and add 0.5% (w / w) NaHCO 3 Boil in the solution for 30min, wash with distilled water to remove NaHCO 3 And sericin, repeat the above steps once, and the degummed silk fibroin is dried at room temperature for later use.

[0050] (2) configure 2wt% sodium hydroxide solution and stir evenly;

[0051] (3) Add degummed tussah silk, and immerse in sodium hydroxide solution with a solid-to-liquid ratio of 1:25g / mL to obtain a mixed solution;

[0052] (4) Put the mixed solution in an ice-water bath at -80°C to freeze and then thaw it, accompanied by mechanical stirring, repeated several times, and obtained a solid suspension after 20 hours;

[0053] (5) washing the water-insoluble matter in the solid sus...

Embodiment 3

[0057] The preparation method of silk protein nanofiber dispersion liquid comprises the following steps:

[0058] (1) Source and degumming of silk protein: castor silk is produced in Zhejiang, China, and castor silk is degummed by the method in Example 2.

[0059] (2) configure 4wt% lithium hydroxide solution, and stir;

[0060] (3) add degummed castor silk, and immerse in the lithium hydroxide solution with a solid-to-liquid ratio of 1: 40g / mL to obtain a mixed solution;

[0061] (4) The mixed solution was frozen and thawed in an ice-water bath at -60°C, accompanied by mechanical stirring, repeated several times, and a solid suspension was obtained after 40 hours;

[0062] (5) washing the water-insoluble matter in the solid suspension to neutrality;

[0063] (6) A 0.3% (w / w) water dispersion system was configured with neutral water-insoluble matter as a raw material and stirred evenly, and the castor silk protein nanofiber dispersion was successfully prepared by ultrasonica...

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Abstract

The invention discloses a preparation method and application of silk protein nanofiber. The preparation method comprises the following steps of adding silk into an alkaline solution to obtain a mixed solution; stirring to obtain a solid suspension; and separating water-insoluble substances in the solid suspension, washing the water-insoluble substances to be neutral, and performing mechanical treatment to obtain the silk protein nanofiber. The silk protein nanofiber has good biocompatibility. The preparation method is simple in process, non-toxic and harmless, the preparation efficiency of the silk protein nanofiber is improved, and a new thought and a new method are provided for efficient utilization of silk protein-based biomass resources.

Description

technical field [0001] The invention relates to a preparation method and application of fiber materials, in particular to a preparation method and application of silk protein nanofibers. Background technique [0002] In order to transform silk from a natural macroscopic structure into a new material with different shapes and characteristics, different solvents (LiBr aqueous solution, CaCl 2 / ethanol / water solution) have successfully dissolved degummed silk and prepared a regenerated silk protein solution, but with the destruction of the fibril structure during the dissolving process of silk fibroin, the nanofibrous structure of regenerated silk is different from that of natural silk, and the silk fibroin biomaterials Degradation of mechanical properties. [0003] Silk nanofibers can retain the nanofiber structure of natural silk protein, and can be used as building blocks or reinforcing components to prepare silk fibroin and materials with excellent performance or functiona...

Claims

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Application Information

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IPC IPC(8): D01F4/02C08J3/03C08L89/00
CPCD01F4/02C08J3/03C08J2389/00
Inventor 范一民石梦月俞娟刘亮
Owner NANJING FORESTRY UNIV
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