Dendrobium-derived exosome-like vesicles as well as preparation and application thereof in improving alopecia

A technology of exosomes and dendrobium, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, drug combinations, etc., to achieve good biocompatibility, high promotional effect, and the effect of improving alopecia

Pending Publication Date: 2021-12-28
COSMAX CHINA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] At present, there is no report on the a

Method used

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  • Dendrobium-derived exosome-like vesicles as well as preparation and application thereof in improving alopecia
  • Dendrobium-derived exosome-like vesicles as well as preparation and application thereof in improving alopecia
  • Dendrobium-derived exosome-like vesicles as well as preparation and application thereof in improving alopecia

Examples

Experimental program
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Embodiment 1

[0034] Example 1: Determination of the content of Dendrobium-derived exosome-like vesicles prepared by differential centrifugation combined with density gradient centrifugation

[0035] For the convenience of understanding, the present invention compares the content of exosome-like vesicles from different sources of Dendrobium. Herb Dendrobium, Dendrobium Butterfly, the parts are fresh stems.

[0036] Steps:

[0037] 1) successively pulverize 100g of above-mentioned 9 kinds of dendrobium fresh stems to obtain dendrobium juice;

[0038] 2) The juice was centrifuged at 4°C, 500×g for 20 minutes to obtain a supernatant. Then, the supernatant was centrifuged again at 4° C. and 2,000×g for 30 minutes to obtain a supernatant.

[0039] 3) After successively adding 0.5 ml of 80% sucrose aqueous solution, 1 ml of 30% sucrose aqueous solution and 32 ml of supernatant liquid to remove impurities in a 35 ml ultracentrifuge tube, the mixture was ultracentrifuged at 4° C. and 80,000×g fo...

Embodiment 2

[0045] Example 2: Preparation of Dendrobium-derived exosome-like vesicles

[0046] The invention provides a method for preparing exosome-like vesicles derived from dendrobium as the active ingredient. The preparation raw material is: fresh stems of Dendrobium candidum (DENDROBIUMOFFICINALE).

[0047] Steps:

[0048] 1) Obtain the dendrobium juice by pulverizing the fresh stem of Dendrobium officinale;

[0049] 2) The juice was centrifuged at 4° C., 1000×g for 10 minutes to obtain a supernatant. Then, the supernatant was centrifuged again at 4° C. and 4,000×g for 20 minutes to obtain a supernatant.

[0050] 3) After successively adding 0.5 ml of 80% sucrose aqueous solution, 1 ml of 30% sucrose aqueous solution and 32 ml of supernatant liquid to remove impurities in a 35 ml ultracentrifuge tube, the mixture was ultracentrifuged at 4° C. and 80,000×g for 4 Hour. After ultracentrifugation, the Dendrobium-derived exosome-like vesicles were located between the 80% sucrose layer...

Embodiment 3

[0053] Example 3: Preparation of Dendrobium-derived exosome-like vesicles

[0054] The invention provides a method for preparing exosome-like vesicles derived from dendrobium as an active ingredient. The preparation raw material is: fresh stems of Dendrobium nobile (DENDROBIUM NOBILE).

[0055] Steps:

[0056] 1) Obtain the dendrobium juice by pulverizing the fresh stem of Dendrobium officinale;

[0057] 2) The juice was centrifuged at 4°C, 500×g for 20 minutes to obtain a supernatant. Then, the supernatant was centrifuged again at 4° C. and 2,000×g for 30 minutes to obtain a supernatant.

[0058] 3) After successively adding 0.5 ml of 80% sucrose aqueous solution, 1 ml of 30% sucrose aqueous solution and 32 ml of supernatant liquid to remove impurities in a 35 ml ultracentrifuge tube, the mixture was ultracentrifuged at 4° C. and 80,000×g for 4 Hour. After ultracentrifugation, the Dendrobium-derived exosome-like vesicles were located between the 80% sucrose layer and the ...

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Abstract

The invention discloses dendrobium-derived exosome-like vesicles as well as preparation and application thereof in improvement of alopecia. The effect of the dendrobium-derived exosome-like vesicles on preventing and improving alopecia is obviously higher than that of a dendrobium extract. The exosome-like vesicle is used as a natural nano-scale vesicle structure, has good biocompatibility and oral safety, can cross a biological barrier, and has a higher promotion effect on transmission of active ingredients compared with common plant extracts, so that the exosome-like vesicle has a good application prospect. The plant-derived exosome-like vesicles can better penetrate through a skin barrier to act on hair follicle stem cells. In addition, the dendrobium exosome-like vesicles are obtained from the dendrobium by adopting a differential centrifugation method in combination with density gradient centrifugation, the purity is further improved, and the effects of preventing and improving the alopecia are improved.

Description

technical field [0001] The invention belongs to the field of fine chemicals, and relates to dendrobium-derived exosome-like vesicles and their preparation and application in improving alopecia, in particular to dendrobium-derived exosome-like vesicles and their preparation and their use in preventing and improving alopecia application. Background technique [0002] Seborrheic alopecia (SA) is the most common clinical hair loss disease. Modern medicine believes that it is an androgen-dependent autosomal dominant polygenic hereditary alopecia. Excessive androgen or increased sensitivity to androgen is the main factor for the occurrence of the disease. The occurrence of SA is related to testosterone (T) and its metabolite Dihydrotestosterone (Dihydrotestosterone, DHT) and androgen receptor (Androgen receptor, AR) levels are directly related to the increase, and 5α-reductase (5α-reductase, 5a-R) plays an important role in it, in addition Long-term mental stress, eating disorde...

Claims

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Application Information

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IPC IPC(8): C12N5/04A61K36/8984A61P17/14A61K135/00
CPCC12N5/04A61K36/8984A61P17/14
Inventor 朱云周秋娜金炫延金荣熙申彦晟金延埈
Owner COSMAX CHINA INC
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