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HPLC fingerprint spectrum detection method and phenolic substance content determination method for amomum tsao-ko

A technology of fingerprint and detection method, which is applied in the field of component analysis of grass fruit samples by fingerprint analysis method of traditional Chinese medicine, so as to achieve the effect of guaranteeing clinical curative effect

Active Publication Date: 2022-01-07
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN104297373 discloses a fingerprint detection method of Tsao Kuo, but this method is also limited to the analysis of volatile oil components, and there is no research on the analysis method of non-gasifiable components in Tsao Kuo based on HPLC, such as phenolic substances

Method used

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  • HPLC fingerprint spectrum detection method and phenolic substance content determination method for amomum tsao-ko
  • HPLC fingerprint spectrum detection method and phenolic substance content determination method for amomum tsao-ko
  • HPLC fingerprint spectrum detection method and phenolic substance content determination method for amomum tsao-ko

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The fingerprint spectrum detection of embodiment 1 Caoguo of the present invention

[0032] 1) Preparation of reference solution

[0033] Protocatechuic acid, protocatechualdehyde, p-hydroxybenzoic acid, gentisic acid, vanillic acid, and p-hydroxyphenylpropionic acid reference substances were accurately weighed respectively, and methanol was added to dissolve and constant volume to make mass concentrations of 3.45, 1.46, 4.72, 107.83, 5.61, 54.36μg / mL mixed reference substance stock solution, set aside.

[0034] 2) Preparation of the test solution

[0035] Take about 5.0 g of Cao Guo powder (passed through a No. 2 sieve), and weigh it accurately. Put in a stoppered Erlenmeyer flask, add 50mL of 70% ethanol accurately, weigh the mass, treat with ultrasonic for 30min, put it to room temperature, make up the lost mass with 70% ethanol, shake well, filter with 0.45μm microporous membrane, take the cont. Filtrate, that is.

[0036] 3) Accurately draw 10 μl each of the re...

Embodiment 2

[0040] Determination of phenolic substance content in Tsaoguo of the present invention in embodiment 2

[0041] 1) Preparation of reference solution

[0042] Protocatechuic acid, protocatechualdehyde, p-hydroxybenzoic acid, gentisic acid, vanillic acid, and p-hydroxyphenylpropionic acid reference substances were accurately weighed respectively, and methanol was added to dissolve and constant volume to make mass concentrations of 3.45, 1.46, 4.72, 107.83, 5.61, 54.36μg / mL mixed reference substance stock solution, set aside.

[0043] 2) Preparation of the test solution

[0044] Take about 5.0 g of Cao Guo powder (passed through a No. 2 sieve), and weigh it accurately. Put in a stoppered Erlenmeyer flask, add 50mL of 70% ethanol accurately, weigh the mass, treat with ultrasonic for 30min, put it to room temperature, make up the lost mass with 70% ethanol, shake well, filter with 0.45μm microporous membrane, take the cont. Filtrate, that is.

[0045]3) Accurately draw 10 μl ea...

experiment example 1

[0050] 1 Instruments and materials

[0051] 1.1 Instrument

[0052] Agilent 1260Infinity II high-performance liquid chromatography (G7114A 1260VWD Detector, LC-12602.4.0.628 chromatographic workstation, Agilent, USA); ME204 1 / 10,000 analytical balance (Mettler-Toledo Instruments Shanghai Co., Ltd.); SB25-12D Ultrasonic cleaning instrument (Ningbo Xinyi Ultrasonic Equipment Co., Ltd.); MiLi-Q pure water instrument (American milipore company).

[0053] 1.2 Materials

[0054] Protocatechualdehyde (batch number: PS020016) and p-hydroxybenzoic acid (batch number: PS010279) reference substances were purchased from Chengdu Pusi Biotechnology Co., Ltd., with a mass fraction of ≥98%. Protocatechuic acid (batch number: MUST-20110310), gentisic acid (batch number: MUST-20082605), vanillic acid (batch number: MUST-20072701), p-hydroxyphenylpropionic acid (batch number: MUST-20091902) were purchased from Chengdu Master Biotechnology Co., Ltd., mass fraction ≥ 98%; methanol (chromatograp...

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Abstract

The invention discloses an HPLC fingerprint spectrum detection method for amomum tsao-ko. The method comprises the following steps of 1) preparing a reference substance solution, 2) preparing a test solution, and 3) respectively sucking the reference substance solution and the test solution, injecting into a high performance liquid chromatograph, and recording chromatograms. According to the detection method, phenolic acid substances including protocatechuic acid, p-hydroxybenzoic acid, gentisic acid, vanillic acid and p-hydroxyphenylpropionic acid and phenolic aldehyde substances including protocatechuic aldehyde in the amomum tsao-ko can be quantitatively detected, 21 common characteristic peaks in the fingerprint spectrum are calibrated, quality control over the amomum tsao-ko is achieved through more comprehensive chemical components, the clinical curative effect of amomum tsao-ko is guaranteed, and the practical popularization and application value is achieved.

Description

technical field [0001] The invention relates to a method for analyzing and evaluating the quality of tsaoko, in particular to analyzing the components of tsaoko samples by adopting a traditional Chinese medicine fingerprint analysis method. Background technique [0002] Tsaoko is the dry and mature fruit of Amomum tsaoko Crevost et Lemaire, a perennial herb of the genus Cardamom in the ginger family. It is mainly distributed in Guizhou, Guangxi, Yunnan and other places. Modern studies have shown that it mainly contains chemical components such as volatile oils, flavonoids, phenolic acids, steroids, and diphenylheptanes. The research on the quality control of traditional Chinese medicine has always been the key to the research and development of traditional Chinese medicine. At present, the quality evaluation of Caoguo mainly focuses on items such as appearance quality and volatile oil content indicators, but this is not enough to explain the internal quality of Caoguo, let a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8631
Inventor 蒲忠慧代敏
Owner CHENGDU MEDICAL COLLEGE