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Method for evaluating quality of atractylodes macrocephala koidz by quantitative analysis of multi-components by single marker

A kind of Atractylodes macrocephala, quality technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of reducing detection costs, etc., and achieve the effect of simple operation, accurate method, and easy mastery

Pending Publication Date: 2022-01-07
ZHEJIANG SHOUXIANGU BOTANICAL DRUG INST CO LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the deficiencies of existing single-component and multi-indicator content determination methods, the present invention provides a method for evaluating the quality of Atractylodes macrocephala with high practicability, simple operation, cost-saving, stable and controllable one-test and multiple-evaluation method. III is the internal reference substance, establish the relative correction factor between Atractylodes lactone III and Atractylodes lactone II, Atractylodes lactone I, and atractylone, and calculate the relative correction factor for Atractylodes lactone II by only measuring the content of Atractylodes lactone III , Atractylodes lactone I and atractylone content, establish a comprehensive and controllable Atractylodes quality evaluation method, which greatly reduces the detection cost, and the detection results are more stable and controllable

Method used

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  • Method for evaluating quality of atractylodes macrocephala koidz by quantitative analysis of multi-components by single marker
  • Method for evaluating quality of atractylodes macrocephala koidz by quantitative analysis of multi-components by single marker
  • Method for evaluating quality of atractylodes macrocephala koidz by quantitative analysis of multi-components by single marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Determination of Atractylodes Lactone III, Atractylodes Lactone II, Atractylodes Lactone I and Atractylone in Atractylodes Rhizoma Atractylodes Rhizome by One Test and Multiple Evaluations

[0040] (1) Material preparation: grind Atractylodes macrocephala into powder, pass through a No. 2 sieve, and set aside;

[0041] (2) Preparation of mixed standard solution: Accurately weigh Atractylodes lactone III, Atractylodes lactone II, Atractylodes lactone I, and atractylodes ketone, place them in 10ml volumetric flasks respectively, add appropriate amount of methanol to dissolve, and set the volume to the mark. Shake well, prepare a single standard solution with concentrations of 0.849mg / mL, 0.898mg / mL, 0.637mg / mL, and 2.73mg / mL, and accurately draw the above four standard solution 0.5, 2, 4, 6, 8 , 10mL, dilute to 100mL with methanol, mix and shake well, as a mixed reference solution, set aside;

[0042] (3) Preparation of the test solution: Accurately weigh 2.00...

Embodiment 2

[0053] Embodiment 2: The influence of extraction solvent on content and stability of Atractylodes macrolide III, Atractylodes lactone II, Atractylodes lactone I and Atractylodes ketone in Atractylodes macrocephala test product solution

[0054] Based on Example 1, the influence of the extraction solvent in the preparation method of the test solution of the present invention on the content and stability of Atractylodes macrolide III, Atractylodes lactone II, Atractylodes lactone I and atractylodes ketone in the Atractylodes macrocephala test solution is studied:

[0055] The solvent methanol in the step (3) of Example 1 is changed to 70% methanol solvent group, 80% methanol solvent group, 90% methanol solvent group, 100% methanol solvent group and 70% ethanol solvent group, and other operating methods remain unchanged Obtain 70% ethanol solvent group Atractylodes Rhizome need testing solution, 70% methanol solvent group Atractylodes Rhizome need testing solution, 80% methanol so...

Embodiment 3

[0056] Example 3: Methodological verification of one test and multiple evaluations

[0057] Based on embodiment 1, the method of the present invention is carried out methodology verification from the following aspects:

[0058] 3.1 Drawing of standard curve

[0059] Precisely draw the mixed standard solution of each concentration in step (2), analyze and measure according to the chromatographic conditions in step (4), record the corresponding peak area, take the mass concentration (μg / mL) as the abscissa (X), the peak The area is the ordinate (Y), and a standard curve is drawn to obtain the linear relationship between Atractylodes III, Atractylodes II, Atractylodes I and atractylone, as shown in Table 3.

[0060] Table 3 Linear relationship of each component

[0061]

[0062] 3.2 Precision test

[0063]Accurately draw the test solution in step (3) respectively, inject samples according to the chromatographic conditions in step (4), continuously inject samples for 6 times...

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Abstract

The invention provides a method for evaluating quality of atractylodes macrocephala koidz by a quantitative analysis of multi-components by single marker, wherein the method comprises the steps: performing quantitative analysis of multi-components by single marker by taking atractylenolide III which is low in cost and easy to obtain as an internal reference substance, and establishing relative retention time and relative correction factors between the atractylenolide III and atractylenolide II, atractylenolide I and atractylenone; and the contents of atractylenolide III, atractylenolide II, atractylenolide I and atractylenone in the atractylodes macrocephala koidz are calculated through correction factors, and the ultra-high performance liquid chromatography is adopted for determination. According to the method, the content of index components is calculated through relative correction factors and chromatographic peak positioning, the content of four components comprising atractylenolide III, atractylenolide II, atractylenolide I and atractylenone in atractylodes macrocephala koidz can be effectively detected at the same time, the cost can be saved, the operation is simplified, the efficiency is improved, the detection sensitivity is high, the stability is good, the determination result is accurate and reliable, and the method is of great significance to quality control of a large amount of atractylodes macrocephala koidz and guarantee of clinical effects of atractylodes macrocephala koidz.

Description

technical field [0001] The invention belongs to the technical field of quality control of medicinal materials, and in particular relates to a method for evaluating the quality of Atractylodes macrocephala with one test and multiple evaluations. Background technique [0002] The ingredients of traditional Chinese medicine are complex and changeable, and their pharmacological activity and clinical efficacy are often the result of the joint action of multiple ingredients. Taking the content of a single component or a certain type of component as an evaluation index usually cannot fully and fully reflect the quality of traditional Chinese medicine. However, to control the quality of traditional Chinese medicine by measuring the content of various components, there are many problems such as scarcity, instability, and high cost of reference substances, which cause great difficulties in detection. Therefore, how to simultaneously determine the content of multiple or multiple medic...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/027G01N2030/8809Y02A50/30
Inventor 李振皓李明焱张国亮余欢欢李振宇史月姣
Owner ZHEJIANG SHOUXIANGU BOTANICAL DRUG INST CO LTD
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