Monoclonal antibody for detecting mouse interleukin 6 as well as preparation method and application thereof

Abstract

The invention provides a monoclonal antibody for detecting mouse interleukin 6 as well as a preparation method and application thereof, and belongs to the technical field of immunoassay. According to the invention, an artificially synthesized mouse interleukin 6 gene is subjected to plasmid recombination, protein expression, animal immunization and screening, and finally, a hybridoma cell strain 20-A4-D5 and a hybridoma cell strain 10-B8-C9 are obtained through subcloning; and an antibody secreted by the hybridoma cell strain 20-A4-D5 is labeled with acridinium ester to obtain a detection antibody, an antibody secreted by the hybridoma cell strain 10-B8-C9 is labeled with biotin to obtain a capture antibody, and the detection antibody and the capture antibody are applied to a chemiluminescence kit for detecting mouse interleukin 6. The kit provided by the invention is high in detection sensitivity, good in precision, wide in linear range and good in antigen reaction specificity, and does not generate cross reaction.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to chemiluminescence immunoassay technology, and in particular to a monoclonal antibody for detecting mouse interleukin-6 and a preparation method and application of the monoclonal antibody. Background technique [0002] Interleukin, also known as interleukin, mouse interleukin 6 (IL-6 or IL6) is a pleiotropic, α-helical, 22-28 kDa phosphorylated and variable glycosylated cytokine that plays a role in acute phase reactions, inflammation , hematopoiesis, bone metabolism and cancer progression. Together with tumor necrosis factor alpha (TNF-α) and interleukin 1 (IL-1), IL-6 drives the acute inflammatory response. IL-6 is almost entirely responsible for the febrile and acute phase responses of the liver and plays a very important role in the transition from acute inflammation to acquired immunity or chronic inflammatory disease. In addition to its functions in inflammation an...

AI Technical Summary

Problems solved by technology

Radioimmunoassay has the disadvantages of short validity period and radioactive contamination

The ELISA method is usually only suitable for manual operation, which is not conducive to the detection of high-throughput automatic detection instruments, and increases the error caused by human factors in the experimental results; and the manual operation steps of the ELISA method are cumbersome, error-prone, and detection sensitivity is also low (usually can only reach the detection limit of ng / mL), and the linear range is narrow

The detection range of immunoradiography is narrow, usually 5-200pg / mL

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