Magnetic molecularly imprinted polymer capable of specifically adsorbing gliadin and preparation method thereof

A magnetic molecular imprinting and gliadin technology, which is applied in the fields of polymer chemistry and protein engineering, can solve the problems of difficulty in maintaining protein activity, complicated operation of instruments and equipment, and inability to separate proteins, so as to inhibit the oxidation or degradation of the Fe3O4 core. Acidic material etching, improved recognition and adsorption capacity, and short saturation time

Pending Publication Date: 2022-01-28
河南省农业科学院农副产品加工研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chromatography and precipitation methods are simple and easy to operate, but they are greatly affected by protein concentration, solution pH and temperature during the separation process, resulting in low purity of the separated protein.
Chromatography and electrophoresis currently have better separation effects, but the equipment is complicated to operate and the separation cost is high; and it is difficult to maintain the activity of the protein during the separation process
In addition, the common disadvantage of these conventional protein separation methods is that they cannot specifically separate proteins.

Method used

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  • Magnetic molecularly imprinted polymer capable of specifically adsorbing gliadin and preparation method thereof
  • Magnetic molecularly imprinted polymer capable of specifically adsorbing gliadin and preparation method thereof
  • Magnetic molecularly imprinted polymer capable of specifically adsorbing gliadin and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A magnetic molecular blotting polymer that can be absorbed by hydraulic protein, the preparation method is as follows:

[0048] 1) Preparation FE 3 O 4 Magnetic microspheres

[0049] 2.70g of FeCl 3 · 6h 2 O dissolve in 80 ml of ethylene glycol, after magnetic force is stirred to completely dissolve, 3.6 g of sodium anhydrous acetate and 2.0 g of polyethylene glycol 2000 were added, and after stirring, the mixed liquid was moved to the hydrothermal reaction kettle, placed in In a heating of 10 h at 205 ° C, the reaction kettle was taken out, and the reaction was cooled; the product obtained from the reaction kettle was taken out, and washed 5 times with ethanol and distilled water, 50 ° C under vacuum dried 12 h, resulting in Fe 3 O 4 Magnetic microspheres.

[0050] 2) Preparation FE 3 O 4 @Sio 2 Magnetic nuclear shell microspheres

[0051] Take step 1) get the FE 3 O 4 The magnetic microspheres were dispersed in 100 mL of ethanol-water (4 / 1, v / v) solvent, and 2.5 ml of c...

Embodiment 2

[0059] A magnetic molecular blotting polymer that can be absorbed by hydraulic protein, the preparation method is as follows:

[0060] 1) Preparation FE 3 O 4 Magnetic microspheres

[0061] 2.70g of FeCl 3 · 6h 2 O Soluble in 80 ml of ethylene glycol, after magnetic force is stirred to completely dissolve, 5.4 g of anhydrous acetate acetate and 1.5 g of polyethylene glycol 2000, stirred thoroughly, the mixed liquid is moved to the hydrothermal reaction kettle, placed in In a heating of 10 h at 205 ° C, the reaction kettle was taken out, and the reaction was cooled; the product obtained from the reaction kettle was taken out, and washed 5 times with ethanol and distilled water, 50 ° C under vacuum dried 12 h, resulting in Fe 3 O 4 Magnetic microspheres.

[0062] 2) Preparation FE 3 O 4 @Sio 2 Magnetic nuclear shell microspheres

[0063] Take step 1) get the FE 3 O 4 The magnetic microspheres were dispersed in 100 mL of ethanol-water (4 / 1, v / v) solvent, and 2.5 ml of concentrated ...

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Abstract

The invention belongs to the technical field of polymer chemistry and protein engineering, and particularly relates to a magnetic molecularly imprinted polymer capable of specifically adsorbing gliadin and a preparation method thereof. The preparation method comprises the following steps: firstly, coating silicon dioxide on the surface of a Fe3O4 magnetic microsphere to obtain a Fe3O4 coated SiO2 magnetic microsphere; then modifying a silicon dioxide shell layer to obtain a double-bond functionalized Fe3O4 (at) SiO2-C = C magnetic microsphere; and preparing an imprinting layer outside the Fe3O4 (at) SiO2-C = C magnetic microspheres, and removing template molecules in the imprinting layer to obtain the magnetic molecularly imprinted polymer capable of specifically adsorbing gliadin. The finally obtained magnetic molecularly imprinted polymer is short in adsorption saturation time of gliadin, high in adsorption capacity and strong in adsorption specificity.

Description

Technical field [0001] The present invention belongs to the field of polymer chemistry and protein engineering, and is specifically involved in a magnetic molecular blotting polymer having a specific adsorption of wheat alcohol proteins and a preparation method thereof. Background technique [0002] With the incidence of food allergies around the world, food allergy has become one of the increasing food safety issues in the 21st century. According to data, the population of various food allergies accounts for about 5% in adults, accounting for 8% in children. According to the International Food Codex Commission (CAC), the eight common foods of peanuts, milk, soybeans, eggs, nuts, fish, shellfish and wheat are 90% of food allergic allergens. Among them, wheat is the world's main food crop, which is the most important part of the daily diet of mankind, but is also one of the eight major food allergens, mainly because wheat contains allergic reactions that can induce allergic reacti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F220/06C08F220/54C08F222/38C08J9/26C08L51/10B01J20/28B01J20/26B01J20/30
CPCC08F292/00C08J9/26B01J20/28009B01J20/268B01J20/28011C08J2351/10C08F220/06C08F220/54C08F222/385
Inventor 张康逸温青玉王宇飞孙东弦李天齐
Owner 河南省农业科学院农副产品加工研究中心
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