Fluorescent probe for detecting copper ions as well as preparation method, detection method and application of fluorescent probe

A fluorescent probe, copper ion technology, applied in the fields of chemical analysis and environmental safety detection, can solve the problems of increased burden on the liver and kidneys, insufficient fluorescence quantum yield, poor metal ion selectivity, etc., and achieves good visual fluorescence change effect, High selectivity and sensitivity, strong photostability effect

Pending Publication Date: 2022-01-28
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although copper ions are essential trace elements for the human body, excessive intake of copper ions will increase the burden on the liver and kidneys, leading to diseases such as Wilson's disease and Alzheimer's disease
[0003] At present, atomic absorption spectrometry and colorimetry are mostly used for the specific detection of copper ions in wastewater and food. These methods have low sensitivity, complicated operation and high cost.
Fluorescent molecular probe technology has received more attention as a new detection technology, but ordinary fluorescent molecular probes have poor selectivity for metal ions in aqueous solution, and the fluorescence quantum yield is insufficient.

Method used

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  • Fluorescent probe for detecting copper ions as well as preparation method, detection method and application of fluorescent probe
  • Fluorescent probe for detecting copper ions as well as preparation method, detection method and application of fluorescent probe
  • Fluorescent probe for detecting copper ions as well as preparation method, detection method and application of fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of fluorescent probe

[0019] 1. Preparation of 1,1,2,2-tetra-(4-carboxy-(1,1-biphenyl))ethylene solution

[0020] Weigh 40mg of 1,1,2,2-tetrakis-(4-carboxy-(1,1-biphenyl))ethylene and dissolve it in 40mL of 0.01M NaOH, repeatedly ultrasonically vortex until completely dissolved, and prepare 1mg / mL The solution.

[0021] Second, the preparation of polyethyleneimine solution

[0022] Weigh 500 mg of polyethyleneimine and dissolve it in 5 mL of ultrapure water, vortex repeatedly until it is completely dissolved, prepare a polyethyleneimine concentration of 100 mg / mL, and dilute to a series of concentrations with ultrapure water.

[0023] 3. Synthesis of fluorescent probes

[0024] Its reaction process is as figure 1 As shown, specifically: Add 1mL of 1,1,2,2-tetrakis-(4-carboxy-(1,1-biphenyl))ethylene solution to 0.9mL ultrapure water, mix well, add to the above solution 0.1 mL of polyethyleneimine solution was added to the solution, sti...

Embodiment 2

[0031] Example 2: Determination of fluorescent probes and 1,1,2,2-tetra-(4-carboxy-(1,1-biphenyl))ethylene in different Cu 2+ Fluorescence emission spectra in concentrations

[0032] Prepare three concentrations of Cu 2+ solution (0, 100 and 1000nM), add 20μL fluorescent probe prepared in Example 1 and 1,1,2,2-tetrakis-(4-carboxy-(1,1-biphenyl))ethylene solution to 300μL Tris Kind of Cu 2+ concentration of the solution, the excitation light is 360nm, and the emission is at 400-800nm. The result is as image 3 As shown, at room temperature, in different Cu 2+ Concentration, the left picture is the emission spectrum of the fluorescent probe, it can be seen that Cu at different concentrations 2+ Concentration, the fluorescence intensity of the fluorescent probe has an obvious response, and the illustration (physical photo) can also be clearly seen that with Cu 2+ As the concentration increases, the fluorescence in the enzyme-labeled wells is significantly quenched; Cu 2+ ...

Embodiment 3

[0033] Embodiment 3: Application of fluorescent probe to draw standard curve for determination of Cu 2+

[0034] Prepare a series of concentrations of Cu 2+ solution (0, 5, 10, 25, 50, 100, 250 and 500nM), take 20 μL of the fluorescent probe prepared in Example 1 and add 300 μL of different Cu 2+ Concentration, by measuring the fluorescence signal, the fluorescence response map (such as Figure 4 shown on the left) and the standard curve (such as Figure 4 shown on the right), the ordinate in the figure is the difference of fluorescence intensity, and the abscissa is Cu 2+ Concentration, the inset is the physical map; it can be found that with Cu 2+ As the concentration increases, the fluorescent signal in the enzyme-labeled wells gradually weakens.

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Abstract

The invention discloses a fluorescent probe for detecting copper ions as well as a preparation method, a detection method and application of the fluorescent probe. The fluorescent probe is formed by uniformly mixing polyethyleneimine and 1, 1, 2, 2-tetra-(4-carboxyl-(1, 1-biphenyl)) ethylene under an ultrasonic condition. By utilizing the properties of polyethyleneimine, on one hand, the polyethyleneimine is used as a complexing agent for enhancing fluorescence of 1, 1, 2, 2-tetra-(4-carboxyl-(1, 1-biphenyl)) ethylene, so that the fluorescence quantum yield of the polyethyleneimine is increased from 1.13% to 39.66%; meanwhile, the chelating agent also serves as a chelating agent for specific detection of Cu < 2 + >. In addition, the synthesized fluorescent probe is mild in preparation condition, simple and convenient in steps, high in solubility in water, capable of meeting the requirement for water solution detection, high in light stability, small in influence of the environment on fluorescence, good in visual fluorescence change effect, high in selectivity and sensitivity to Cu < 2 + > and wide in application prospect in an environment monitoring system.

Description

technical field [0001] The invention belongs to the fields of chemical analysis and environmental safety detection, and in particular relates to a fluorescent probe for detecting copper ions, a preparation method, a detection method and an application thereof. Background technique [0002] The toxicity and existing forms of heavy metal ions are the main problems that endanger people's health. Many countries are polluted by heavy metal ions. Copper ions are heavy metal ions that often enter the table through the food chain in wastewater. Although copper ions are essential trace elements for the human body, excessive intake of copper ions will increase the burden on the liver and kidneys, leading to diseases such as Wilson's disease and Alzheimer's disease. [0003] At present, atomic absorption spectrometry and colorimetry are mostly used for the specific detection of copper ions in wastewater and food. These methods have low sensitivity, complicated operation and high cost....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06G01N21/643G01N21/6447C09K2211/1007C09K2211/14
Inventor 邓省亮赖卫华张干章钢刚苏柳贺伟华邱小忠
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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