Paracoccus communis YBH-X with dimethylacetamide degradation capability and application
A technology of dimethylacetamide and YBH-X, which is applied in the field of microorganisms and can solve problems such as refractory biodegradation, environmental and human toxicity
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[0027] The preparation method of described bacterium-containing suspension comprises the following specific steps:
[0028] (1) Slant culture: inoculate Paracoccus YBH-X with dimethylacetamide degrading ability on the slant medium, and cultivate it at 30-38° C. for 1-3 days to obtain slant bacteria; the slant medium The final concentration is composed of: K 2 HPO 4 1500mg·L -1 , KH 2 PO 4 500mg·L -1 , NaCl 1000mg·L -1 , DMAC 500mg·L -1 , MgSO 4 ·7H 2 O200mg·L -1 , solvent is water, pH value 7.0-8.0, agar 18-20g L -1 ;
[0029](2) Seed culture: pick colonies from the slant and inoculate them into the seed medium, and cultivate them at 30-38°C for 18-24h to obtain the seed liquid; the final concentration of the seed medium consists of: NaCl 10g L -1 , yeast extract powder 5g·L -1 , peptone 10g·L -1 , the solvent is water, the pH value is 7.0-8.0;
[0030] (3) Fermentation: inoculate the seed solution to the fermentation medium with an inoculation amount of 1% vol...
Embodiment 1
[0034] Example 1: Isolation, purification and identification of Paracoccus communis YBH-X
[0035] 1. Isolation and purification of Paracoccus communis YBH-X
[0036] Paracoccus communis YBH-X was screened from the aerobic pool sludge of a pharmaceutical wastewater treatment plant in Taizhou, Zhejiang. The specific steps are as follows:
[0037] (1) Sampling: Sampling at multiple points from the sludge in the aerobic tank of a pharmaceutical wastewater treatment plant in Zhejiang, as a raw material for screening Paracoccus communis YBH-X, which has the ability to degrade dimethylacetamide;
[0038] (2) Strain isolation: Take an appropriate amount of activated sludge in the aerobic tank, let it stand for 2 hours, take 10 mL of supernatant and inoculate it into a 250 mL culture bottle containing 100 mL of enriched culture solution, and shake it on a constant temperature shaker at 30°C and 160 rpm 24h, repeated 3 times, the final concentration components of the enriched culture ...
Embodiment 2
[0046] Example 2 The preparation process of the bacterial suspension containing Paracoccus YBH-X with dimethylacetamide degradation ability as active ingredient
[0047] The preparation process of the bacterium-containing suspension:
[0048] (1) Slant culture: inoculate Paracoccus YBH-X with dimethylacetamide degrading ability on the slant medium, and cultivate it at 30°C for 3 days to obtain slant bacteria; the final concentration of the slant medium consists of :K 2 HPO 4 1500mg·L -1 , KH 2 PO 4 500mg·L -1 , NaCl 1000mg·L -1 , DMAC 500mg·L -1 , MgSO 4 ·7H 2 O 200mg·L -1 , solvent is water, pH value 7.0, agar 18g L -1 ;
[0049] (2) Seed culture: pick colonies from the slant and inoculate them into the seed medium, and cultivate them at 30°C for 18 hours to obtain the seed liquid; the final concentration of the seed medium consists of: NaCl 10g L -1 , yeast extract powder 5g·L -1 , peptone 10g·L -1 , the solvent is water, the pH value is 7.0;
[0050] (3) F...
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