Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of fluorescent biosensor for rapid detection of Listeria monocytogenes

A biosensor and sensor technology, applied in fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems of easy false positive results, high design requirements, complicated operation, etc., and achieve easy on-site instant detection and strong anti-interference ability , to overcome the effect of low sensitivity

Pending Publication Date: 2022-01-28
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common nucleic acid amplification methods such as qPCR, mPCR, loop-mediated isothermal amplification, etc., but the operation is complex, requiring professionals and special instruments, and the design of primers and probes is demanding and difficult, and false positive results are prone to occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of fluorescent biosensor for rapid detection of Listeria monocytogenes
  • Construction method of fluorescent biosensor for rapid detection of Listeria monocytogenes
  • Construction method of fluorescent biosensor for rapid detection of Listeria monocytogenes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Fabrication of Fluorescent Biosensors

[0021] (1) Preparation of UCNPs

[0022] 1) Weigh 0.1164g YCl respectively 3 ·6H 2 O, 0.0892g GdCl 3 ·6H 2 O, 0.062g YbCl 3 ·6H 2 O and 0.006g ErCl 3 ·6H 2 O was dissolved in 4mL of methanol solution, sonicated until completely dissolved, and then poured into a three-necked flask rinsed with a little methanol (the three-necked flask must be kept clean, if necessary, it can be soaked overnight in aqua regia); then poured into 6mL of oleic acid and 14 mL of 1-octadecene.

[0023] 2) Under continuous magnetic stirring, the above mixture was placed under nitrogen protection condition, heated to 160°C, kept for 30min, and then cooled to about 50°C.

[0024] 3) Weigh 0.2964g NH respectively 4 F and 0.2g NaOH were dissolved in 20mL methanol solution, and ultrasonicated for about 15min until completely dissolved; then, under continuous stirring, the solution was added dropwise to the above flask solution. The mixture was the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fluorescent biosensor constructed based on a double-site recognition strategy and a fluorescent inner filter effect. An up-conversion nanoparticle (UCNPs) mediated fluorescent biosensor is constructed, and is used for rapid and sensitive detection of Listeria monocytogenes. The method comprises the following steps: preparing an MNPs-Van magnetic nano probe by taking vancomycin (Van) as a first recognition molecule; and taking a biotinylated aptamer as a second recognition molecule and combining thesecond recognition molecule with internalized protein A on the cell wall of the listeria monocytogenes. Two recognition molecules are used for respectively targeting different sites on target bacteria, so that the target bacteria are specifically captured, and a sandwich type compound (MNPs-Van / Listeria monocytogenes / aptamer) is formed. A HRP-TMB enzyme catalysis system is introduced through a horse radish peroxidase marker (HRP-SA) to generate a blue substance, and the fluorescence intensity of the UCNPs is reduced by combining the fluorescence inner filter effect of the UCNPs, so that the quantitative detection of the listeria monocytogenes is realized.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and analysis. Specifically, it involves the construction of a fluorescent biosensor based on a two-site recognition strategy and a fluorescent inner filter effect, thereby realizing a new method for rapid detection of Listeria monocytogenes in food. Background technique [0002] "Food is the most important thing for the people, and food safety is the first priority." Food safety is one of the livelihood issues that ordinary people are most concerned about. Food-borne diseases caused by food-borne pathogenic microorganisms are important public health problems, and they are also the key monitoring objects of food safety in various countries. There are reports of food poisoning and food hygiene incidents every year, especially pathogenic microorganisms, which are extremely unfavorable for the healthy and orderly development of the country. From the data analysis of foodborne disease o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 潘磊庆李悦屠康陈敏彭菁王振杰
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com