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Bacterium capable of secreting riboflavin and resisting heavy metal copper and cadmium and application thereof

A heavy metal, riboflavin technology, applied in Citrobacter JPG1 and its application fields, can solve problems such as affecting the growth of plants and crops, affecting the quality of the ecological environment and sustainable agricultural development, bone pain disease, etc.

Pending Publication Date: 2022-02-01
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, cadmium will affect the growth of plants and crops, lead to a decrease in soil production capacity, and can also be transferred to the human body through the food chain, endangering health. Long-term drinking of cadmium-contaminated water can cause "bone pain disease", which can be fatal in severe cases
Copper is an essential trace element for humans and plants, but excessive copper will accumulate in humans, animals and plants, affect normal physiological and biochemical processes, and cause harm. Excessive copper in water and soil will also affect the ecological environment Quality and agricultural sustainability

Method used

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  • Bacterium capable of secreting riboflavin and resisting heavy metal copper and cadmium and application thereof
  • Bacterium capable of secreting riboflavin and resisting heavy metal copper and cadmium and application thereof
  • Bacterium capable of secreting riboflavin and resisting heavy metal copper and cadmium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Scanning electron micrograph, growth curve and riboflavin synthesis gene of embodiment 1 bacterial strain JPG1

[0019] (1) Scanning electron microscope of strain JPG1

[0020] Take the strain JPG1 cultured at 30°C and 120rpm for 48h, centrifuge at 8000rpm for 5min, discard the supernatant, add 10mL of PBS buffer, wash 3 times at 8000rpm for 5min, add 10mL of 2.5% glutaraldehyde solution, fixed overnight. After washing with PBS buffer for 3 times, dehydrate with graded ethanol (30%, 50%, 70%, 80%, 90% and 100%) step by step, dehydrate for 15 min at each concentration, centrifuge at 8000 rpm for 5 min, and dehydrate with 100% ethanol 2 times, 20min / time, and finally replaced with pure tert-butanol twice, 30min each time. After the processed samples were cooled in a -80°C refrigerator for 1 h, they were vacuum-dried in a freeze dryer for 24 h. Spray gold coating, observe the morphology of strain JPG1 with scanning electron microscope ( figure 1 ). It can be seen that...

Embodiment 2

[0027] Heavy Metal Tolerance of Example 2 Strain JPG1

[0028] Preparation of Citrobacter JPG1 bacterial suspension: Inoculate the bacterial liquid taken out of the -80°C refrigerator into 100mL LB liquid medium, shake at 30°C and 120rpm to the logarithmic growth phase, centrifuge at 8000rpm for 5min, discard Remove the supernatant, rinse with sterile water 3 times and resuspend to adjust the OD 600 = 0.3 spare.

[0029] (1) Tolerance of strain JPG1 to heavy metal copper

[0030] The bacterial suspension of Citrobacter JPG1 was inoculated into 100mL LB liquid medium containing copper ion concentrations of 0, 64, 96, 128 and 160mg / L according to the inoculum size of 1%, and the temperature was kept at 30°C and 120rpm Culture, at 0, 2, 3, 5, 8, 11, 23, 29, 35, 47 and 59 hours, measure its OD 600 . The experimental results show( image 3 ), Citrobacter JPG1 can grow normally under a certain copper ion concentration, indicating that Citrobacter JPG1 has a certain tolerance to...

Embodiment 3

[0033] Example 3 Removal of heavy metal copper and cadmium ions in wastewater by bacterial strain JPG1

[0034] with CuCl 2 2H 2 O to prepare simulated wastewater containing copper ion concentration of 20mg / L, CdCl 2 2.5H 2 O prepared 100 mL of LB liquid medium containing cadmium ion concentration of 5 mg / L in the simulated wastewater, but the actual measured initial concentration of copper ion was 16.9 mg / L, and the initial concentration of cadmium ion was 4.3 mg / L. Transfer the bacterial suspension of Citrobacter JPG1 to the liquid LB medium containing heavy metal copper and cadmium ions according to the inoculation amount of 1%, and cultivate it at 30°C and 120rpm for 96h under constant temperature conditions, and take 10mL of the bacterial solution every 24h, at 8000rpm After centrifugation for 5 min, the supernatant was digested with nitric acid, filtered through a 0.22 μm filter membrane, and the concentration of copper and cadmium ions in the supernatant was measured...

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Abstract

The invention discloses a strain of citrobacter sp. JPG1, which has the capability of secreting riboflavin and also has the capability of removing heavy metal copper and cadmium ions in wastewater. The strain JPG1 is preserved in the China General Microbiological Culture Collection Center in December 2016, and the preservation number of the strain is CGMCC No.13480. The citrobacter JPG1 not only has related genes secreting riboflavin, but also has heavy metal copper and cadmium resistance, can remove heavy metal copper ions and cadmium ions in wastewater at the same time, and has great application potential in the aspect of removing heavy metal in wastewater.

Description

technical field [0001] The invention belongs to the technical field of waste water microbial treatment, and relates to a Citrobacter sp. JPG1 which can not only secrete riboflavin but also remove heavy metal copper and cadmium ions in waste water and its application. Background technique [0002] In recent years, the discharge of heavy metal ions from industrial wastewater such as metal processing, mining and electroplating industries has seriously endangered environmental health. Wastewater often contains more than a single heavy metal ion, and usually multiple heavy metal ions exist at the same time. Among them, cadmium will affect the growth of plants and crops, lead to a decrease in soil productivity, and can also be transferred to the human body through the food chain, endangering health. Long-term drinking of cadmium-contaminated water can cause "bone pain disease", and in severe cases, it can be fatal. Copper is an essential trace element for humans and plants, but ex...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20
Inventor 王小雨时露
Owner NORTHEAST NORMAL UNIVERSITY
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