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Efficient cutting propagation method for huperzia serrata under natural conditions

A technology of Huperzinia serrata and natural conditions is applied in the field of high-efficiency cutting propagation of Huperzia serrata under natural conditions, which can solve the problems of high price of Huperzine A, low production cost of seedlings, and high seedling breeding coefficient, so as to improve the success rate and Effects of reproduction coefficient, shortening the germination time of new leaves, and increasing reproduction coefficient

Pending Publication Date: 2022-02-11
ZHEJIANG SCI-TECH UNIV
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Problems solved by technology

At present, there are still certain technical difficulties in the artificial breeding of Huperzia serrata. Although there have been many years of research reports on its breeding, there have been no reports of large-scale successful breeding and cultivation. The gradual depletion of wild resources in Tajikistan has led to continuously high prices of Huperzine A. It is necessary to develop a rapid breeding method with high seedling breeding coefficient, short growth cycle and low seedling production cost. On the basis of effective protection of wild Huperzine serrata resources, Carry out sustainable industrialization development

Method used

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  • Efficient cutting propagation method for huperzia serrata under natural conditions

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Embodiment 1

[0023] The entire cutting propagation process is carried out under natural conditions from April to May, and the specific steps are as follows:

[0024] 1) Cutting substrate preparation: prepare a mixed substrate with a formula of river sand: humus: organic fertilizer = 4:4:2, mix evenly, and put the prepared mixed substrate into a seedling container tray with a hole diameter of 5 cm. The height is 6 cm, and the sterilized water is poured thoroughly; the substrate and the surrounding environment are sprayed and disinfected with 500 times carbendazim aqueous solution;

[0025] 2) Rooting hormone configuration: rooting hormones are hormones indole butyric acid (IBA), α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BA), 1L sterilized water, which contains indole butyric acid (IBA ) is 200mg / L, α-naphthaleneacetic acid (NAA) is 100mg / L, 6-benzylaminopurine (6-BA) is 100mg / L, and the mass ratio is: indolebutyric acid (IBA): α-naphthaleneacetic acid ( NAA): 6-benzylaminop...

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Abstract

The invention discloses an efficient cutting propagation method for huperzia serrata under natural conditions. The method comprises the following steps of (1) preparing a substrate, (2) preparing rooting hormone, (3) selecting cutting slips, (4) treating the cutting slips, (5) cutting, and (6) management after cuttage. According to the method, under natural conditions, the cutting medium components and the pH value are strictly treated, the composition and proportion of rooting hormones are optimized and screened, and the conditions of cutting slips are optimized and selected and segmented, so that the rooting time of cutting seedlings and the germination time of new leaves are shortened, and the survival rate and the propagation coefficient of the cutting seedlings are increased; therefore, the purpose of improving the cutting propagation efficiency of huperzia serrata is achieved, the survival rate and the rooting rate of the cutting slips reach 96.1% and 95.5% respectively, and the propagation coefficient reaches 8.6. The problems that the huperzia serrata cuttage propagation rate is low, rooting is not prone to occurring in cuttage, the seedling rate is low, and cuttage growth is slow are effectively solved, and wild population recovery and large-area industrial application are facilitated.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a high-efficiency cutting propagation method for Huperz serrata under natural conditions. Background technique [0002] Huperzia serrata (Thunb.ex Murray) Trev, also known as Melaleuca pagoda, King of Help, Gold Buchang, Sanzhi, Serrata, belongs to the fern Huperzia serrata Huperzia. Folks often use the whole herb to treat bruises, bruises, swelling and pain, schizophrenia and other diseases. Huperzine A (hupenineA, hupA) is a natural plant alkaloid extracted from the traditional Chinese medicine Huperzia plant Melaleuca (Huperzia serrata) by domestic scholars in the 1980s. It is a potent, reversible and highly selective second-generation plant alkaloid. Acetylcholinesterase inhibitors are currently the most successful drugs for the treatment of Alzheimer's disease (senile dementia) developed in China, and they can also enhance the learning and memory of norma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G22/00A01G24/12A01G24/20A01N43/38A01N37/10A01N43/90A01P21/00
CPCA01G2/10A01G22/00A01G24/12A01G24/20A01N43/38A01N37/10A01N43/90
Inventor 汪得凯庞礡陈彤彤黄紫怡沈红军贾巧君梁宗锁
Owner ZHEJIANG SCI-TECH UNIV
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