Method for reducing dosage of fish meal in aquatic feed by adding ingestion growth regulating peptide

A technology for aquatic feed and growth regulation, applied in the field of polypeptide function research, can solve the problems of shortage of fish meal and increased cost of aquatic feed, etc.

Pending Publication Date: 2022-02-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of fishmeal shortage and increasing cost of aquatic feed, and provide a method for reducing the amount of fi...

Method used

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  • Method for reducing dosage of fish meal in aquatic feed by adding ingestion growth regulating peptide
  • Method for reducing dosage of fish meal in aquatic feed by adding ingestion growth regulating peptide
  • Method for reducing dosage of fish meal in aquatic feed by adding ingestion growth regulating peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Ingestion of Growth Regulating Peptide Spray Feed Technology

[0041] The ingestion growth regulating peptide was chemically synthesized, the purity was more than 95%, and its core amino acid sequence was YPVKPENPGED (the amino acid sequence is shown in SEQ ID NO: 1). The polypeptide feeding growth regulating peptide is added to the feed by spraying, and the method of preparing the spraying liquid and the spraying method are as follows:

[0042] (1) Take out the protein dry powder from the -80°C refrigerator and centrifuge it in a small centrifuge for 3 minutes. After the dry powder is completely placed at the bottom of the tube, slowly add 1 mL of double-distilled water along the tube wall, and fully vortex and centrifuge 3 times to prepare a concentration of is x mg / mL stock solution (placed at -20°C).

[0043] (2) Calculate the working fluid concentration according to the required amount. Taking a fish in each group, the average body weight is b kg, and t...

Embodiment 2

[0046] Example 2 The effect of feeding growth-regulating peptides on the growth of jellyfish fed with feeds with different fish meal content

[0047] 1. Experimental method

[0048]Two kinds of gem bass feeds containing 10% fish meal and 20% fish meal were prepared, and the total protein mass concentration of the two feeds was 40%. The feed formula is shown in Table 1. The ingested growth regulatory peptides were added to the feed by spraying as in Example 1.

[0049] Gem perch with a body weight of 14±2 grams and a uniform body weight were selected for grouping, and there were 4 treatments in total, namely, 10% fish meal by mass, 10% fish meal by mass + feeding growth regulating peptide, 20% fish meal by mass, and 20% fish meal by mass concentration. + ingestion of growth regulating peptides, each treatment set 3 replicates, a total of 12 groups, 100 fish in each group, fed with 10% fish meal group diet, the adaptation period was 14 days, the feeding amount was 4% of the bod...

Embodiment 3

[0075] Example 3 Effects of ingestion of growth-regulating peptides on the growth of tilapia fed with feeds with different fishmeal content

[0076] 1. Experimental method

[0077] Three tilapia feeds containing 0% fish meal, 3% fish meal and 10% fish meal were prepared, and the total protein mass concentration of the three feeds was 30%. The feed formulations are shown in Table 4. The ingested growth regulatory peptides were added to the feed by spraying as in Example 1.

[0078] Tilapia groups with a uniform body weight of 10±2 grams were selected, and there were 6 treatments in total, namely, 0% fish meal, 0% fish meal + feeding growth regulating peptide, 3% fish meal, and 3% fish meal + Feeding growth regulating peptide, 10% fish meal by mass concentration and 10% fish meal by mass concentration + feeding growth regulating peptide, each treatment set 3 replicates, a total of 18 groups, 100 tails in each group, fed with 0% fish meal group feed, given No feed additives wer...

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Abstract

The invention discloses an artificially synthesized ingestion growth regulating peptide for reducing the additive amount of animal protein in aquatic feed. The tilapia mossambica and the jewel perches are fed by spraying ingestion growth regulating peptides on aquatic feed. The ingestion growth regulating peptide can improve the growth performance of tilapia mossambica and jewel perches, the ingestion growth regulating peptide is added into low-fish-meal feed, the mass increasing rate and the specific growth rate of the tilapia mossambica and the jewel perches can be increased to the level of a high-fish-meal group, and therefore the effect of reducing the fish meal content in the feed is achieved, and the water quality pollution caused by excessive addition of fish meal is reduced.

Description

technical field [0001] The invention belongs to the technical field of polypeptide function research, and in particular relates to a method for reducing the amount of fish meal in aquatic feed by adding dietary growth regulating peptide. Background technique [0002] Tilapia, commonly known as African crucian carp, Fushou fish, and tilapia, belong to the order Perciformes, Perciformes, Cichlididae, and Oreochromis. Tilapia has low cost, good economic benefits, and simple and easy breeding technology. It is an excellent breeding species that is vigorously developed and promoted by countries all over the world, and is known as one of the main sources of animal protein in the future. Australian gem perch (Scortum barcoo), also known as gem perch, gem spot, jade perch, belongs to the order Perciformes, the family Scortum, the genus Scortum, the meat is delicious, the content of unsaturated fatty acids is high, and the internal organs and intermuscular spines are less. , rapid g...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K20/147A23K40/30A23K10/22
CPCA23K50/80A23K20/147A23K40/30A23K10/22
Inventor 李文笙俞筱筝
Owner SUN YAT SEN UNIV
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