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Muscle building and therapeutic application of tetrathiomolybdate

A technology of tetrathiomolybdate and muscle, which is applied in the fields of application, muscular system diseases, and the function of food ingredients, etc., to achieve the effect of increasing muscle mass, promoting differentiation, and increasing the cross-sectional area of ​​muscle fibers

Active Publication Date: 2022-02-15
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the whole, although tetrathiomolybdate has been involved in muscle-related research, so far, there is still no technology involving tetrathiomolybdate that can promote muscle growth, and there is no relevant theory that can be based on current research. It is technically extended or deduced that tetrathiomolybdic acid can promote muscle growth

Method used

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  • Muscle building and therapeutic application of tetrathiomolybdate
  • Muscle building and therapeutic application of tetrathiomolybdate
  • Muscle building and therapeutic application of tetrathiomolybdate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This example provides a demonstration example of the muscle-enhancing function of tetrathiomolybdate at the animal level.

[0051] A healthy mouse model was used to observe the muscle-increasing effect of tetrathiomolybdate (ammonium tetrathiomolybdate was specifically used in this example) administration on the mouse model, and to analyze the molecular biological aspects of the tested animals. Indicator changes.

[0052] The experimental process is as follows:

[0053] 1) Experimental animals: male C57BL / 6J mice, SPF grade, weighing 24±1g, purchased from Hunan Slake Jingda Experimental Animal Co., Ltd., randomly divided into tetrathiomolybdate treatment group and control group, 6 in each group Only. The animals were raised in SPF cages, the ambient temperature was 22°C, and the humidity was suitable;

[0054] 2) Feeding operation: During the animal adaptation stage, the body weight is recorded at 14:00 every day, the change of food weight every 24 hours is recorded ...

Embodiment 2

[0110] This example provides a demonstration example of tetrathiomolybdate achieving muscle-increasing function at the animal cell level.

[0111] The C2C12 mouse myoblast model was used to observe the effect of tetrathiomolybdate on the differentiation of mouse myoblasts, and the changes of various indicators at the molecular biology level of the model were analyzed.

[0112] The experimental process is as follows:

[0113] 1) Cell culture: Dilute C2C12 mouse myoblasts to 1×10 with medium (DMEM high glucose) containing 10% fetal bovine serum 5 cells / ml, seeded into 6-well plates, at 37°C, 5% CO 2 Culture in the environment; when the cell density reaches about 80-85%, remove the medium in the well, and add the medium containing 2% horse serum (DMEM high sugar) to induce myotube differentiation; the experimental group is dissolved by adding DMSO Tetrathiomolybdate (due to previous experiments found that tetrathiomolybdate has an impact on the function of C2C12 cells under the...

Embodiment 3

[0122] This example provides a demonstration example of the function of tetrathiomolybdate in improving the animal's motor ability and learning and memory ability.

[0123] Using a healthy mouse model, observe the performance of the mouse model in the Morris water maze test after administration of tetrathiomolybdate, and analyze the changes in the indicators representing the exercise ability and learning and memory ability of the mice (see Figure 13 ~ Figure 15 ).

[0124] Depend on Figure 13 It can be seen that the tetrathiomolybdate treatment in this example increases the swimming speed of the mice, and the faster the speed, the better the exercise ability of the mice.

[0125] Depend on Figure 14 and 15 It can be seen that the tetrathiomolybdate treatment in the present embodiment shortened the latency period before the mice found the platform, and increased the number of times the mice passed through the hidden platform. These two indicators all showed that the learn...

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Abstract

The invention discloses application of tetrathiomolybdate in preparation of products or drugs capable of increasing muscles, improving muscle functions, enhancing athletic ability and preventing or treating muscle system related diseases. The molecular general formula of the tetrathiomolybdate is X<+>MoS<4><2->Y<+>, the positions of ionic bonds of four sulfur ions are interchangeable, and X<+> and Y<+> are the same or different cations. The invention provides application of tetrathiomolybdate in preparation of products or drugs capable of increasing muscle, improving muscle functions, enhancing exercise ability and preventing or treating related diseases of a muscle system, the tetrathiomolybdate not only can increase muscle mass, increase muscle protein content and increase muscle fiber cross sectional area, but also can promote differentiation of myoblasts and activate an mTOR muscle building signal channel, does not cause liver function damage in the animal experiment process or cause body fat increase, and is remarkable in effect.

Description

technical field [0001] The invention belongs to the application of thiomolybdate, in particular to the application of thiomolybdate in the fields of increasing muscle, improving muscle function, enhancing exercise capacity, preventing or treating muscular system-related diseases and the like. Background technique [0002] Muscle is the most abundant protein storage pool in the human body and plays an important role in regulating body health and glucose and lipid metabolism (Sartori et al., Nat Commun 2021, 12, 330). However, under normal circumstances, human skeletal muscle continues to attenuate with age. Generally, after the age of 50, skeletal muscle decreases by an average of 1% to 2% per year, and 30% muscle loss will affect its normal function, resulting in sarcopenia. (sarcopenia) occurrence. Under multiple pathological conditions (e.g. altered endocrine function, chronic disease, inflammation, insulin resistance, and nutritional deficiencies), accelerated loss of mu...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61P21/00A23L33/10A23L2/52
CPCA61K33/24A61P21/00A23L33/10A23L2/52A23V2002/00A23V2200/316
Inventor 李国林鲁思宇魏芳
Owner HUNAN NORMAL UNIVERSITY
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