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Application of 3,5-dimethoxytoluene in nematode poisoning and nematode poisoning agent containing 3,5-dimethoxytoluene

A technology of dimethoxytoluene and nematodes, applied in the directions of nematicides, applications, biocides, etc., can solve the problems of high toxicity of pesticides and environmental pollution, and achieve strong insecticidal effect, high toxicity and good effect. Effect

Active Publication Date: 2022-02-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide the application of 3,5-dimethoxytoluene in killing nematodes and the nematode poisoning agent containing this component, so as to solve the current problem Some pesticides have high toxicity, environmental pollution, and residues in crops, etc., to improve the safety of agricultural production

Method used

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  • Application of 3,5-dimethoxytoluene in nematode poisoning and nematode poisoning agent containing 3,5-dimethoxytoluene
  • Application of 3,5-dimethoxytoluene in nematode poisoning and nematode poisoning agent containing 3,5-dimethoxytoluene
  • Application of 3,5-dimethoxytoluene in nematode poisoning and nematode poisoning agent containing 3,5-dimethoxytoluene

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Indoor Toxicity Determination of 3,5-Dimethoxytoluene to Caenorhabditis elegans

[0033] The nematodes used in the experiment were the model organism Caenorhabditis elegans L4 nematodes, which were cultured and propagated in a constant temperature and humidity biochemical incubator at 20°C. Adopt the lysis method to obtain worm eggs, the specific method is as follows: the Caenorhabditis elegans adults in the plate are rinsed with M9 buffer and placed in a 1.5mL centrifuge tube, centrifuged at 4000rpm to retain the precipitate, after washing 3 times, add 1mL of lysate ( 1% sodium hypochlorite 1mL + 5mM sodium hydroxide 1mL, mix well before use), shake vigorously until the worms are broken under the microscope, centrifuge to discard the supernatant, rinse several times with M9, and incubate in buffer at 20°C Overnight, the synchronized nematodes can be obtained the next day, and the obtained synchronized nematodes are cultured in NGM medium to the L4 stage. Syn...

Embodiment 2

[0043] Example 2 Effect of 3,5-dimethoxytoluene on the number of eggs laid by Caenorhabditis elegans

[0044] The method described in Example 1 was used to cultivate Caenorhabditis elegans, and the collected L3 stage nematodes were transferred to a 96-well plate without adding 5-FUDR, and the dosage of the drug solution was set to 24h LC 50 1 / 10, 1 / 100, 1 / 1000 of , that is high, medium and low doses. After the nematodes grew to the L4 stage, they were transferred to an empty NGM medium, and one nematode was cultured in each NGM medium, and transferred every 24 hours until the end of the nematode oviposition period and the total number of nematode reproduction was recorded. Each group had at least 8 nematodes, the experiment was repeated 3 times, and the mixed solution of Tween 80 and K-medium was used as the blank control. The data results are shown in Table 2.

[0045] Table 2 The effect of 3,5-dimethoxytoluene on the number of eggs laid by Caenorhabditis elegans

[0046] ...

Embodiment 3

[0048] Example 3 Effects of 3,5-dimethoxytoluene on the growth and development of Caenorhabditis elegans

[0049] Adopt the method described in Example 1 to carry out the cultivation of Caenorhabditis elegans, and the collected L4 stage nematodes are transferred to a 96-well plate added with 12.5 mg / L of 5-FUDR (5-fluorouridine deoxynucleoside). The dose is set to 1 / 10, 1 / 100, 1 / 1000 of 24h LC50, that is, high, medium and low doses. Pick 30 nematodes into each hole, after exposure for 48 hours, place them in an incubator at 60°C for 10 minutes, kill the nematodes with hot steam to straighten the nematodes, then observe and take pictures under a stereomicroscope, using the microscope’s own The body width and length of the nematode were measured with a micrometer. The experiment was repeated three times, and the mixed solution of Tween 80 and K-medium was used as the blank control. The data results are shown in Table 3.

[0050] Table 3 Effects of 3,5-dimethoxytoluene on body w...

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Abstract

The invention discloses application of 3,5-dimethoxytoluene in nematode poisoning and a nematode poisoning agent containing the 3,5-dimethoxytoluene, and belongs to the technical field of nematode prevention and control. According to the invention, the natural compound 3,5-dimethoxytoluene extracted from plants is used for poisoning and killing nematodes, so that a relatively strong insecticidal effect is achieved. Compared with a common chemical pesticide, the 3,5-dimethoxytoluene has the characteristics of low toxicity, good effect, wide source, safety and the like, can be used as an ideal environment-friendly pesticide, well solves the problems of high toxicity, environmental pollution, residues in crops and the like of the existing pesticide, and improves the safety of agricultural production.

Description

technical field [0001] The invention relates to the technical field of nematode control, in particular to the application of 3,5-dimethoxytoluene in killing nematodes and a nematode poisoning agent containing the component. Background technique [0002] According to the morphological characteristics and feeding habits of nematodes, nematodes in soil can be divided into plant-parasitic nematodes, bacteria-eating nematodes, fungi-eating nematodes and omnivorous / predatory nematodes. Soil nematodes speed up the turnover of microbial biomass, nutrients and soil organic matter by changing and regulating the composition of microbial community structure, while directly participating in the nutrient utilization of plants and microorganisms. Among them, plant parasitic nematodes have caused huge losses to the agricultural economy. When plant parasitic nematodes invade host tissues, they will absorb nutrients from plants and secrete some toxins or enzymes to induce pathological changes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/14A01P5/00
CPCA01N31/14Y02A50/30
Inventor 王国健张瑞杨佳彤王英平焉学倩李伟
Owner JILIN AGRICULTURAL UNIV
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