Method for removing benzopyrene in hyperforin perforatum extract by using solvent

Abstract

The invention belongs to the technical field of plant extract purification, and discloses a method for removing benzopyrene in a hyperforin perforatum extract by using a solvent. The method comprises the following steps: adding a hyperforin perforatum raw material into an extraction tank, and performing reflux extraction with ethanol to obtain an extracting solution; blending the extracting solutions, filtering by using a plate-and-frame filter press, and carrying out vacuum concentration on the filtrate to recover ethanol so as to obtain an extracted concentrated solution; completely dissolving the extracted concentrated solution with ethanol, and extracting the solution with a solvent accounting for 1 / 3 of the volume of the liquid; and performing vacuum concentration on the extracted ethanol solution, adding purified water according to half of the volume of the concentrated solution, repeating the operations twice to remove solvent residues, continuing concentration, discharging the concentrated solution, and performing spray drying while the solution is hot. The process is simple to operate, is suitable for industrial production, and can effectively solve the problem that benzopyrene in the extract exceeds the standard due to raw material pollution. The content of four polycyclic aromatic hydrocarbons in the extract treated by the method is 13.9 ppb, the content of benzopyrene is 0.79 ppb, and the content of the effective component hypericin is slightly increased compared with the content of hypericin extracted without the solvent.

Description

technical field [0001] The invention belongs to the technical field of plant extract purification, and in particular relates to a method for removing benzopyrene from Hypericum perforatum extract by using a solvent. Background technique [0002] At present, Hypericum perforatum raw materials are polluted by the atmosphere, soil, water and other external environments, and benzopyrene is enriched in plants. According to the test data, the four polycyclic aromatic hydrocarbons in the raw material of Hypericum perforatum are 32.9ppb, of which benzopyrene is 4.8ppb, The final four polycyclic aromatic hydrocarbons in the obtained extract are 200ppb, and benzopyrene is 35.1ppb, seriously exceeding the standard, which affects the safety of product use and cannot reach the EU standard. [0003] The existing patent CN201710226153.1 "A Process for Removing Benzopyrene from Oil" discloses that the oil is eluted with an aqueous solution of DNA. The benzopyrene in the oil intercalates wit...

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Problems solved by technology

[0007] (1) The prior art is to remove benzopyrene in vegetable oil, and there is no report on the removal of benzopyrene from plant extracts

[0008] (2) Hypericum perforatum extract cannot be separated with water after being dissolved in a solvent. Adding alkali will destroy its active ingredient hypericin, and adding an adsorbent will adsorb the active ingredient hypericin, resulting in a reduction in product content. Therefore None of the existing technologies are feasible

[0009] The difficulty of solving the above problems and defects is: firstly, the extract of Hypericum perforatum can not be dissolved in oil, and the current method is to remove benzopyrene from oil; secondly, the method of using alkali refining, alkali will cause the extract of Hypericum perforatum The destruction of active ingredients such as hypericin in the product will affect the efficacy of the drug and cause the product to be unusable; adding adsorbent activated carbon in the process of adsorbing benzopyrene, the activated carbon will adsorb the active ingredients such as hypericin, making the product content Reduced, not up to the export standard, so it cannot be used

[0010] The meaning of solving the above problems and defects is: if the above method is used for processing, the final result is that the content of the extract is not up to the standard or the benzopyrene components cannot be removed

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