Wild melastoma plant chromosome slide preparation method based on FISH hybridization

A technique for producing chromosomes and chromosomes, which is applied in the field of cytogenetics, can solve problems such as FISH hybridization research, cell membrane rupture, and low production efficiency, and achieve good chromosome dispersion, reduce toxic effects, and improve production efficiency.

Pending Publication Date: 2022-02-18
FUJIAN INST OF TROPICAL CROPS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the phenomenon that the chromosomes of the genus Metonia are small and easy to overlap, people's understanding of the molecular cytogenetic mechanism of the genus Melastoma is limited.
[0003] At present, the existing production methods on the market need to pre-process the chromosome samples of the genus Metonia and then fix them. , prone to incomplete cell separation, cell membrane rupture, and chromosome overflow, making it impossible to carry out subsequent FISH hybridization studies

Method used

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  • Wild melastoma plant chromosome slide preparation method based on FISH hybridization

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Effect test

Embodiment 1

[0023] A kind of FISH hybridization-based method for producing chromosomes of Mulax genus plants, the method for producing chromosomes specifically comprises the following steps:

[0024] (1) Sampling: Carry out hydroponic cultivation of the plants of the genus of the genus, when the roots of the plants of the genus of the genus are grown to 1 cm, take 0.5 cm of the root tip as a sample of the chromosome, wherein, the hydroponic method is: adopt young stems of the plants of the genus of the genus of the genus of the genus Put it into clean water for cultivation, the hydroponics time is 25 days, and the hydroponics season is early summer;

[0025] (2) Fixing: the above-mentioned chromosomal sample is fixed with a fixative, wherein the fixative is a mixture of ethanol, glacial acetic acid and chloroform, and the mass ratio of ethanol, glacial acetic acid, and chloroform in the fixative is 5:3: 2. The fixed time is 24h;

[0026] (3) Hypotonicity: Take the chromosome sample out o...

Embodiment 2

[0030] A kind of FISH hybridization-based method for producing chromosomes of Mulax genus plants, the method for producing chromosomes specifically comprises the following steps:

[0031] (1) Sampling: Carry out hydroponic cultivation of the plants of the genus of the genus of the genus, when the roots of the plants of the genus of the genus of the genus of the genus of the genus Peony grow to 1.5 cm, take a root tip of 0.5 cm as a sample of the chromosome. The sections are put into clear water for cultivation, the hydroponics time is 35 days, and the hydroponics season is the beginning of summer;

[0032] (2) Fixing: the above-mentioned chromosomal sample is fixed with a fixative, wherein the fixative is a mixture of ethanol, glacial acetic acid and chloroform, and the mass ratio of ethanol, glacial acetic acid, and chloroform in the fixative is 5:3: 2. The fixed time is 24h;

[0033] (3) Hypotonicity: Take the chromosome sample out of the fixative solution and put it into 0...

Embodiment 3

[0037] A kind of FISH hybridization-based method for producing chromosomes of Mulax genus plants, the method for producing chromosomes specifically comprises the following steps:

[0038] (1) Sampling: Carry out hydroponic cultivation of the plants of the genus of the genus, when the root system of the plants of the genus of the genus of the genus of the genus of the genus of the genus Peony grows to 2 cm, take a root tip of 0.5 cm as a sample of the chromosome, wherein, the hydroponic method is: adopt the young stems of the genus of the genus of the genus of the genus of the genus of the genus, Put it into clear water for cultivation, the hydroponics time is 45 days, and the hydroponics season is early summer;

[0039] (2) Fixing: the above-mentioned chromosomal sample is fixed with a fixative, wherein the fixative is a mixture of ethanol, glacial acetic acid and chloroform, and the mass ratio of ethanol, glacial acetic acid, and chloroform in the fixative is 5:3: 2. The fixe...

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Abstract

The invention discloses a common melastoma plant chromosome slide preparation method based on FISH hybridization, and relates to the technical field of cytogenetics, the chromosome slide preparation method specifically comprises the following steps: (1) sampling; (2) fixing; (3) hypotonic treatment; (4) enzymolysis; and (5) flaking. The common melastoma plant chromosome slice obtained by adopting the chromosome slice preparation method disclosed by the invention has the advantages of multiple division phases, thorough cell wall dissociation, better chromosome dispersion and clean and clear background, and it is convenient to carry out karyotype analysis research of common melastoma plant chromosomes and subsequent FISH hybridization research of common melastoma plants; a foundation is laid for development and utilization of common melastoma plant germplasm resources and research on cytology and molecular cytology, the stationary liquid can enable mitosis cells to stay in the early and middle stages, pretreatment is not needed, the toxic effect of a pretreatment liquid on the cells is reduced, pretreatment and immobilization are combined together, and the flaking efficiency is effectively improved.

Description

technical field [0001] The invention relates to the technical field of cytogenetics, in particular to a FISH hybridization-based method for preparing chromosomes of plants of the genus Melastolo. Background technique [0002] Chromosome research is the basic research in the field of genetics, and is the cornerstone and catalyst of many research fields. The wild peony plants have high ornamental value and high medicinal value, and have broad development prospects. Due to the small chromosomes of the plants of the genus Melastolithia, which are easy to overlap and so on, it limits people's understanding of the molecular cytogenetic mechanism of the genus Melastolithus. [0003] At present, the existing production methods on the market need to pre-process the chromosome samples of the genus Metonia and then fix them. , prone to incomplete cell separation, cell membrane rupture, and chromosome overflow, making it impossible to carry out subsequent FISH hybridization studies. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 何雪娇余智城林秀香陈振东
Owner FUJIAN INST OF TROPICAL CROPS
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