Method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of organic acid and pinellia, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problem of inability to analyze the types of organic acid components
Pending Publication Date: 2022-02-18
HEFEI NORMAL UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The 2015 edition of "Chinese Pharmacopoeia" records that the determination of the organic acid content of Pinellia adopts the potentiometric titration method, which is calculated based on the content of succinic acid for quality control; but this method can only roughly reflect the content of the organic acid components of Pinellia, and cannot analyze the organic Types of Acid Components
At present, there are few reports on the organic acid components of Yaoluoping Pinellia
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0031] Example 1: Extraction and Detection of Organic Acid Components of Pinellia Pinellia and Yaoluoping Pinellia
[0032] 1. Instruments and reagents
[0033] (1) Instruments: ultra-high performance liquid chromatography UPLC (SHIMADZU Nexera X2) and tandem mass spectrometer MS / MS (Applied Biosystems 4500QTRAP), high-speed universal pulverizer.
[0034] (2) Reagents: The tubers of Pinellia and Yaoluoping Pinellia were provided by the School of Life Sciences, Hefei Normal University. Ethanol, acetonitrile, formic acid and ethyl acetate were of chromatographic grade, and other reagents were of analytical grade.
[0035] 2. Material pretreatment
[0036] Freshly collected Pinellia tubers and Pinellia tubers of Yaoluoping were washed with clean water, sliced, dried in an oven at 105°C for 15 minutes, and then dried at 60°C until constant weight (48h). Subsequently, after grinding into powder with a high-speed universal pulverizer, pass through a 60-mesh sieve.
[0037] 3. Extra...
Embodiment 2
[0057] Example 2: Screening of Different Organic Acid Components of Pinellia and Yaoluoping Pinellia
[0058] 1. Quantitative analysis of organic acid components
[0059] Using the software Analyst 1.6.3 to process the mass spectrometry data, obtain the total ion chromatogram (TIC) of the metabolites of Pinellia and Yaoluoping Pinellia. The characteristic ions of each substance were screened out by a triple quadrupole, and the chromatographic peaks were integrated and corrected with MultiaQuant software to complete the quantitative analysis of organic acid components.
[0060] 2. Screening of differential organic acid components
[0061] Comparing the chromatographic peak integration (peak area) of different organic acid components of Pinellia and Pinellia yaoluoping, the components with a difference of more than 2 times or less than 0.5 times are the screened differential organic acid components.
[0062] Depend on figure 1 and figure 2 It can be seen that the peak shape...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid, and belongs to the field of chemical component analysis of traditional Chinese medicines. The method comprises the following steps: using 75% ethanol for heating reflux extraction of total organic acid components of pinellia ternate and Pinellia yaoluopingensis, using ethyl acetate for repeated extraction, removing impurities, and obtaining an organic acid extracting solution; detecting the extracting solution by using UPLC-MS / MS, qualitatively analyzing the organic acid components by using the standard substance database, totally identifying 109 organic acid components, performing quantitative detection by using a multi-reaction detection mode MRM of triple quadrupole mass spectrometry, and screening 91 organic acid components with significant content difference in combination with a difference multiple greater than 2 times or less than 0.5 times. The invention provides an excellent technical means for evaluating the quality of pinellia ternate and Pinellia yaoluopingensis by using organic acid, and has important application value in quality control and variety identification of pinellia medicines.
Description
technical field [0001] The invention belongs to the field of analysis of chemical components of traditional Chinese medicines, and more specifically relates to a method for evaluating the quality of pinellia and yaoluoping pinellia based on organic acids. Background technique [0002] Pinellia ternate is a perennial herbaceous plant of the genus Pinellia in the family Araceae, mainly distributed in Hubei, Henan, Anhui, Shandong, Sichuan and other places; it is used as medicine with tubers, and has the functions of antitussive, expectorant and antiemetic. , sedative, hypnotic, anti-tumor and other effects, it is a bulk medicinal material commonly used in our country. Yaoluoping Pinellia (Pinelliayaoluopingensis) is an endemic species in Yaoluoping National Nature Reserve in Anhui Province. It has similar efficacy with Pinellia, but its medicinal effect is faster than that of Pinellia, which may be related to its chemical composition. [0003] Studies have shown that organic ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more