Probiotic composition as well as preparation method and application thereof

A composition and probiotic technology, applied in the field of microorganisms, can solve problems such as difficulty in reconstruction, imbalance of skin flora, and damage

Active Publication Date: 2022-02-25
山东锦鲤生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of antibiotics in the above methods is effective, but in addition to eliminating beneficial bacteria, they also have the risk of eliminating beneficial bacteria, sensitization, and potential adverse events. May cause an imbalance in the skin...

Method used

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  • Probiotic composition as well as preparation method and application thereof
  • Probiotic composition as well as preparation method and application thereof
  • Probiotic composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Isolation and Identification of Bacterial Strains

[0049] Make MC agar medium: 5g soybean peptone, 5g beef extract, 5g yeast extract, 20g glucose, 20g lactose, 10g calcium carbonate, 15g agar, 5ml 1% neutral red solution, 1000ml distilled water, pH 6, 121℃ Sterilize for 15 minutes.

[0050] Make MRS liquid medium: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween-80 lml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g, 1000ml distilled water, pH 6.4, sterilized at 121°C for 15min.

[0051] The sample in this example comes from the natural pulp of sauerkraut, which is aseptically sampled in a sterilized sampling tube filled with 5ml of glycerin, and frozen at -20°C after retrieval. When separating, redissolve the sample, take 1g in 9ml physiological saline, shake and mix well, take the supernatant and streak it on the MC plate, after 48 hours of constant...

Embodiment 2

[0061] Example 2: Research on the concentration change of the probiotic composition and a single bacterial strain cultured for 16 hours

[0062] Adjust the bacterial concentration of the activated probiotic composition of the present invention, SEUNEU-101, SEUNEU-102 bacterial liquid to OD600 of 0.1, take 100 μl and inoculate it into 1ml MRS, cultivate it in a 37°C incubator for 16 hours, and use spectroscopic The number of bacteria was measured with a luminance meter OD600nm.

[0063] result:

[0064] After 16 hours of incubation, if figure 1 As shown, the concentration change results of probiotic composition and single bacterial strain cultured for 16 hours, SEUNEU-101, SEUNEU-102, probiotic composition OD600 detection results were 3.0, 3.2, 5.2 respectively, indicating that the co-culture of the two strains is more effective than the single strain The growth rate of the culture is faster, and the two strains have the effect of promoting the proliferation of each other. ...

Embodiment 3

[0065] Example 3: Bacteriostasis research of probiotic composition Staphylococcus aureus

[0066] 1. Preparation of probiotic composition bacterial liquid and single bacterial liquid:

[0067] The 1:1 probiotic composition of activated SEUNEU-101 and SEUNEU-102, SEUNEU-101, and SEUNEU-102 bacteria were cultured with MRS in a 37°C incubator for 18 hours, and the number of bacteria was measured using a spectrophotometer OD600nm. Adjust the concentration of probiotic composition, SEUNEU-101, SEUNEU-102 bacterial solution to 2×10 9 cells / mL, centrifuge to obtain the supernatant to obtain the supernatant; after autoclaving at 121°C for 30 min, the inactivated supernatant is obtained.

[0068] 2. Preparation of Staphylococcus aureus liquid:

[0069] Cultivate broth medium in a shaker at 140r / min at 37°C overnight, use a spectrophotometer OD600nm to measure the number of bacteria, and adjust the concentration of lactic acid bacteria to 1×10 8 cells / mL.

[0070] 3. Inhibition of...

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Abstract

The invention provides a probiotic composition as well as a preparation method and application thereof, and belongs to the technical field of probiotic microorganisms. The probiotic composition comprises lactobacillus plantarum SEUNEU-101 and lactobacillus fermentum SEUNEU-102, the lactobacillus plantarum SEUNEU-101 and the lactobacillus fermentum SEUNEU-102 are both preserved in the China Center for Type Culture Collection, the preservation number of the lactobacillus plantarum SEUNEU-101 is CCTCC M 20211279, and the preservation number of the lactobacillus fermentum SEUNEU-102 is CCTCC (China Center For Type Culture Collection) M 20211280. The composition has the effects of regulating human flora balance, improving the skin state, promoting skin repair and relieving skin inflammation, especially self-inflammatory diseases in dermatological significance, and can be used for pharmaceuticals, food, skin care products or cosmetics.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a probiotic composition, a preparation method and an application thereof. Background technique [0002] As the largest organ of the human body, the skin is home to a wide range of microorganisms. The skin constitutes a habitat of various forms such as recessed parts and specialized gaps, helping the widely distributed microorganisms to survive. Microbes form a symbiotic relationship with humans as hosts, and the skin microbiota plays an important role in the host. This is not only because of the skin microbiota's ability to resist the adhesion and development of skin pathogens, but also because of their ability to talk to and interact with the immune system. Moreover, probiotics can also act as modulators to restore the balance of the skin microbiota when dysbiosis occurs at the skin level. [0003] In the past decade, the use of new technologies has facilitated the taxo...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P17/00A61P31/04A61K8/99A61Q19/08A61Q17/00A23L33/135
CPCA61K35/747A61P17/00A61P31/04A61K8/99A61Q19/08A61Q17/005A23L33/135A61K2800/5922A23V2002/00A61K2800/85A61K2800/522A61K2300/00A23V2200/318A23V2250/206Y02A50/30
Inventor 陈奕兴靖培培杨雨孙夏慧王熠郭青青
Owner 山东锦鲤生物工程有限公司
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