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Protective liquid for bone marrow stem cells as well as preparation method and application of protective liquid

A technology for bone marrow stem cells and protective solution, applied in the field of stem cells, can solve the problems of difficult to achieve rapid transport of bone marrow stem cells, limited application of bone marrow stem cells, low cell survival rate, etc. Effect

Pending Publication Date: 2022-03-01
李敬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For bone marrow stem cells that require transportation, cryopreservation with liquid nitrogen requires better transportation equipment, and it is not easy to achieve rapid transport of bone marrow stem cells
In recent years, various preservation solutions that can preserve bone marrow stem cells at low temperature have also appeared one after another, but there are generally various problems, such as short storage time and low cell survival rate, which limit the application of bone marrow stem cells

Method used

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  • Protective liquid for bone marrow stem cells as well as preparation method and application of protective liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A protective solution for bone marrow stem cells, characterized in that it consists of the following raw materials in parts by weight: 80 parts of 0.9% sodium chloride injection, 1.5 parts of jujube saponin A, 3 parts of meglumine, 2 parts of cordycepin, transparent 6 parts of sodium hyaluronate, 0.7 parts of vitamin B2.

[0029] The preparation method of the above-mentioned protective solution for bone marrow stem cells comprises the following steps: adding jujuboside A, meglumine, cordycepin, sodium hyaluronate, and vitamin B2 to 0.9% sodium chloride injection, mixing evenly, and passing through 0.22 µm membrane filter, that is.

[0030] For the application of the above-mentioned bone marrow stem cell protection solution, divide the protection solution into sterile tubes and pre-cool it to 4°C in the refrigerator, then add P3 bone marrow stem cells at a cell density of 1×10 6 cells / mL, and kept in a refrigerator at 4°C for 72 hours.

Embodiment 2

[0032] A protective solution for bone marrow stem cells, characterized in that it consists of the following raw materials in parts by weight: 75 parts of 0.9% sodium chloride injection, 1 part of jujube saponin A, 2 parts of meglumine, 1 part of cordycepin, hyaluronic acid Sodium nitrate 5 parts, vitamin B2 0.5 parts.

[0033] The preparation method of the above-mentioned protective solution for bone marrow stem cells comprises the following steps: adding jujuboside A, meglumine, cordycepin, sodium hyaluronate, and vitamin B2 to 0.9% sodium chloride injection, mixing evenly, and passing through 0.22 µm membrane filter, that is.

[0034] For the application of the above-mentioned bone marrow stem cell protection solution, divide the protection solution into sterile tubes and pre-cool it to 6°C in the refrigerator, then add P3 generation bone marrow stem cells with a cell density of 3×10 6 cells / mL, and kept in the refrigerator at 6°C for 72 hours.

Embodiment 3

[0036] A protective solution for bone marrow stem cells, characterized in that it consists of the following raw materials in parts by weight: 85 parts of 0.9% sodium chloride injection, 2.5 parts of jujube saponin A, 4 parts of meglumine, 5 parts of cordycepin, hyaluronic acid 8 parts of sodium bicarbonate, 0.8 parts of vitamin B2.

[0037] The preparation method of the above-mentioned protective solution for bone marrow stem cells comprises the following steps: adding jujuboside A, meglumine, cordycepin, sodium hyaluronate, and vitamin B2 to 0.9% sodium chloride injection, mixing evenly, and passing through 0.22 µm membrane filter, that is.

[0038] For the application of the above bone marrow stem cell protection solution, the protection solution was divided into sterile tubes and pre-cooled to 6°C in the refrigerator, and then P3 generation bone marrow stem cells were added with a cell density of 5×10 6 cells / mL, and kept in a refrigerator at 4°C for 72 hours.

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Abstract

The invention discloses a protective solution for bone marrow stem cells. The protective solution is prepared from the following raw materials: 0.9% sodium chloride injection, jujuboside A, meglumine, cordycepin, sodium hyaluronate and vitamin B2. According to the protection liquid for the bone marrow stem cells, jujuboside A, meglumine and cordycepin are added to achieve a synergistic effect, the bone marrow stem cells can be stored for 72 h at the temperature of 4-6 DEG C, the cell viability is 89% or above, scientific research and clinical use requirements can be met, and convenience is provided for temporary storage and transportation of the bone marrow stem cells. The proliferation capacity of the bone marrow stem cells preserved by adopting the protective solution is not influenced, and the preserved cells can be continuously used for proliferation and differentiation and the like. The invention further provides a preparation method of the bone marrow stem cell protection liquid, the process is simple, operation is easy, and commercialization of products is facilitated. The invention further provides application of the protective liquid, the protective liquid is used for preservation of the bone marrow stem cells, the protective liquid is safe and non-toxic, preservation and transportation are convenient, and guarantee is provided for clinical application of the bone marrow stem cells.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a protection solution for bone marrow stem cells and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of adult stem cells derived from mesoderm with high self-renewal ability and multilineage differentiation potential. MSCs widely exist in tissues or organs such as bone marrow, fat, muscle, skin, amniotic fluid, and umbilical cord. They have strong proliferation and differentiation capabilities, and low immunogenicity, so they have broad application prospects. [0003] Bone marrow mesenchymal stem cells (BMSCs) are a kind of pluripotent stem cells in the bone marrow, which have all the common characteristics of stem cells, that is, self-renewal and multi-directional induction differentiation capabilities. With the characteristics of strong proliferation ability and no rejection of autologous transplantation, it is wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李敬宋天青
Owner 李敬
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