Effect of exosome in thoracic aortic dissection/aneurysm

A thoracic aorta and exosome technology, applied in the field of biomedicine, can solve the problems of high mortality, high recurrence rate, and many postoperative complications

Pending Publication Date: 2022-03-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, acute TAAD is mainly treated with surgical thoracotomy or endovascular repair, but the mortality rate during the operation is high, and the postoperative complications and recurrence rate are high.

Method used

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  • Effect of exosome in thoracic aortic dissection/aneurysm
  • Effect of exosome in thoracic aortic dissection/aneurysm
  • Effect of exosome in thoracic aortic dissection/aneurysm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 The acquisition of exosomes

[0044] ① Uniformly inoculate UMSC cells in a logarithmic growth phase and in a good growth state in a 10 cm dish, centrifuge fetal bovine serum at a speed of 110,000 g for 7 hours to remove exosomes previously present in the serum, and use 10% exosome-free α-MEM culture medium of fetal bovine serum. The culture was continued for 48 hours, and when the cell density reached 90%, the old medium supernatant was collected, and the cells were subcultured.

[0045] ② Centrifuge the supernatant collected above at a speed of 2000g for 30min to remove dead cells and debris.

[0046] ③Use a straw to absorb the new supernatant, transfer it to a new 50mL centrifuge tube, add 1 / 2 volume of the supernatant PEG4000 exosome isolation reagent, shake it up and down, mix well, and extract at 4°C overnight.

[0047] ④After 24 hours, set the temperature of the centrifuge to 4°C, the speed to 13000g, and the time for 1 hour. After the centrifugation, ...

Embodiment 2

[0050] Embodiment 2 Construction of mouse thoracic aortic dissection / aneurysm model

[0051] Treat 3-week-old, C57BL / 6J male mice with β-aminopropionitrile (BAPN) drinking water, weigh BAPN powder, dissolve it in sterile double-distilled water, and the concentration of BAPN in the first and second weeks is 6mg / mL , the concentration of BAPN increased to 8mg / mL in the third and fourth weeks.

Embodiment 3

[0052] Embodiment 3 ultrasonic measurement diameter

[0053] Mice were anesthetized by respiratory inhalation with 2% isoflurane gas. After successful anesthesia, the skin was prepared on the chest and abdomen of the mice, and the hair on the body surface was removed to reduce the impact on the ultrasound images. The mice were placed in the supine position on the echocardiography platform, the limbs were fixed with adhesive tape on the flat-panel electrocardiogram monitoring position, the ultrasonic coupling agent was applied to the extremities, and the heated ultrasonic coupling agent was evenly applied to the chest and abdomen of the mouse. Using the MS400C ultrasonic probe, fix the ultrasonic probe above the echocardiography platform and adjust it down to a suitable position to avoid exerting too much pressure on the thoracic and abdominal cavity of the mouse while maintaining a clear image in the thoracic and abdominal cavity. Find the long-axis section of the heart along...

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Abstract

The invention relates to the field of biomedicine, in particular to an effect of an exosome in thoracic aortic dissection / aneurysm. The survival rate of a normal mouse group within 30 days is 100%, the survival rate of an aortic dissection / aneurysm group established by using a BAPN drug to induce mice begins to be reduced at the beginning of the third week, the total survival rate is maintained at 50% at the fourth week of an experimental period, and on the basis, the exosome group is injected, so that the survival rate of the mice is slightly reduced after the third week, and the survival rate of the mice is maintained at 50% at the beginning of the third week. When the experiment period is 4 weeks, the survival rate is maintained at 70%. According to the data, the occurrence of thoracic aortic dissection / aneurysm can be obviously delayed after the exosome is injected.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the role of exosomes in thoracic aortic dissection / aneurysm. Background technique [0002] Thoracic aortic dissection / aneurysm (TAAD) is an aggressive vascular disease requiring early diagnosis and effective treatment. However, due to the specific vascular structure and stenosis of the lesion site, there is currently no effective treatment for TAAD. TAAD refers to the permanent expansion of the entire thickness of the aortic wall, and the diameter of the lumen exceeds 50% of the normal to form an aortic aneurysm. With the impact of blood flow, the inner wall of the aorta is damaged, and the high-speed blood flow tears the intima to form a dissection. The annual incidence of thoracic aortic dissection / aneurysm is about 7-9 cases / 100,000. If the rupture is not treated in time, the mortality rate will be as high as 50%-68% within 48 hours, and 90% within 3 months. The annual cas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28C12N5/0775A61P35/00
CPCA61K35/28C12N5/0665C12N5/0668A61P35/00C12N2509/00
Inventor 李杨欣刘冯沈振亚张瑜
Owner SUZHOU UNIV
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