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Limonin compound, preparation method and application of limonin compound as tobacco mosaic virus resisting medicine

A technology of limonoids and compounds, which is applied in the field of pesticides and can solve the problems of unseen extraction of limonoids

Active Publication Date: 2022-03-01
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, so far, there is no report of extracting limonoids from Rehmannia frondosa in the prior art

Method used

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  • Limonin compound, preparation method and application of limonin compound as tobacco mosaic virus resisting medicine
  • Limonin compound, preparation method and application of limonin compound as tobacco mosaic virus resisting medicine
  • Limonin compound, preparation method and application of limonin compound as tobacco mosaic virus resisting medicine

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preparation example Construction

[0058] The present invention also provides a preparation method of the limonoid compound described in the above technical scheme, comprising the following steps:

[0059] Reflux extracting the ethanol aqueous solution and the whole plant of Rhizoma Rehmanniae Compound Leaf, and collecting the extract;

[0060] Concentrating the extract to obtain extract;

[0061] resuspending the extract in water to obtain a crude extract;

[0062] The crude extract is extracted with ethyl acetate to obtain an ethyl acetate extract;

[0063] The ethyl acetate extract was separated by normal phase silica gel column chromatography through the first gradient elution, the procedure of the first gradient elution was: sequentially using 90vol% petroleum ether-10vol% ethyl acetate, 70vol% petroleum ether -30vol% ethyl acetate, 50vol% petroleum ether-50vol% ethyl acetate, 30vol% petroleum ether-70vol% ethyl acetate, 70vol% dichloromethane-30vol% methanol, 50vol% dichloromethane-50vol% methanol, 30vo...

Embodiment 1

[0141] The preparation method of limonin A, B and the compound having the structure shown in formula II comprises the following steps:

[0142] 1) Using 95% ethanol aqueous solution to reflux extract the whole plant of Rehmannia compound leaf, and collect the extract; concentrate the extract to obtain an extract; resuspend the extract in water to obtain a crude extract Extraction liquid; the whole plant of Rehmannia rhizome compound leaf is collected from Xingyi, Guizhou; the whole plant of Rehmannia rhizome compound leaf is dried and crushed to obtain a crushed product, which is mixed with an aqueous ethanol solution for reflux extraction; the particle size of the crushed product is 80~ 100 mesh, the temperature of reflux extraction is 50°C; the number of reflux extraction is 3 times; based on the mass of crushed material as 9kg, the volume of ethanol aqueous solution used for each reflux extraction is 15L; during the process of decompression concentration, extract And recove...

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Abstract

The invention provides limonin compounds, a preparation method and application of the limonin compounds as tobacco mosaic virus resisting drugs, and belongs to the technical field of pesticides. The invention provides limonin compounds as shown in a formula I structure. The limonin compound has a good prevention effect, and the anti-TMV activity of the limonin compound is related to reduction of expression of tobacco mosaic virus coat protein (TMV-CP) and replication of TMV-CP genes and increase of related defensive enzyme activity (phenylalanine ammonialyase, peroxidase, superoxide dismutase and natural polyphenol oxidase). Meanwhile, the expression of the NtHsp70-1 and the expression of the NtHsp70-261 are down-regulated, and it is further shown that inhibition of the limonin compound on TMV infection is further related to reduction of the content of the NtHsp70-1 and the content of the NtHsp70-261.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a limonoid compound, a preparation method and its application as an anti-tobacco mosaic virus drug. Background technique [0002] Munronia henryi Harms, also known as dwarf Tuo Tuo, small ground coptis, belongs to the genus Munronia of the family Meliaceae, about 6 species, produced in the south-central, southwestern and Hainan Island. Born in humid places under forests at an altitude of 1000-1400 meters, it is distributed in Guizhou, Guangxi, Yunnan and other places. The whole plant of Rehmannia compound leaf can be used as medicine for bruises, rheumatoid arthritis, stomach pain, flatulence and abdominal pain, cold and fever, and malaria. However, so far, there is no report on the extraction of limonoids from Rehmannia frondosa in the prior art. Contents of the invention [0003] In view of this, the object of the present invention is to provide a limonoid compound, a pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/14A61K31/365A61P31/12A01N43/08A01P1/00
CPCC07D407/14A61P31/12A01N43/08Y02A50/30
Inventor 晏英汤磊王丹张雄彭明友
Owner GUIZHOU MEDICAL UNIV
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