Application of miR-29a-3p in preparation of toxic diffuse goiter diagnosis product

A 1.mir-29a-3p, goiter technology, applied in the field of biomedicine, can solve the problems of deficiency, arrhythmia, easy misdiagnosis as heart disease, etc., and achieve the effect of stable structure and high abundance

Pending Publication Date: 2022-03-01
ZHENJIANG NO 1 PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many patients have no obvious early clinical symptoms, and severe clinical symptoms and complications have already appeared when the diagnosis is made, such as diffuse goiter, infiltrating exophthalmos, and hypermetabolic syndrome, etc. Related specific indicators make the clinical diagnosis of GD face many difficulties
Due to the lack of specific diagnostic markers, some GDs are often difficult to distinguish from diseases such as simple goiter, Hashimoto's thyroiditis, and subacute thyroiditis in the hyperthyroid stage. Elderly patients with hyperthyroidism are often atypical, with symptoms of weight loss, anorexia, apathy, and heart rhythm. Abnormalities, etc., are easily misdiagnosed as heart disease, nausea and tumors, etc., and their treatment is also facing great challenges

Method used

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  • Application of miR-29a-3p in preparation of toxic diffuse goiter diagnosis product
  • Application of miR-29a-3p in preparation of toxic diffuse goiter diagnosis product
  • Application of miR-29a-3p in preparation of toxic diffuse goiter diagnosis product

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Embodiment 1

[0017] 1, materials and reagents

[0018]

[0019] 2, the main solution is formulated

[0020] PBS buffer: 8.0 g / L of sodium chloride, 0.2 g / L of potassium chloride, sodium hydrogen phosphate 1.42 g / L, 0.24 g / L of phosphate dihydrogen, adjusting pH between 7.2-7.4.

[0021] 3, experimental method

[0022] 3.1 Separate peripheral blood single nuclear cells

[0023] (1) Potential gun, large tunue, FICOLL-HYPAQUE separation, phosphate buffer (PBS), glass centrifuge tube, 1.5MLEP tube, test tube rack, etc., etc., ultraviolet light irradiation 30 minutes.

[0024] (2) Collecting Graves disease and healthy volunteers in the peripheral blood, about 2 ml, 25 ° C, 500 g of centrifugation for 10 minutes, collecting the upper plasma to clean 1.5 ml EP tube, put in -80 The low temperature refrigerator was mixed at ° C, and the remaining blood cells were diluted with PBS to 2 mL mixed.

[0025] (3) Add 2 ml FICOLL-HYPAQUE separation liquid from the center tube, each centrifuge tube, ...

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Abstract

The invention discloses application of miR-29a-3p in preparation of a toxic diffuse goiter diagnosis product, and belongs to the technical field of biological medicine. Detection proves that the miR-29a-3p has expression difference in peripheral blood mononuclear cells of a patient suffering from the Graves disease and a healthy control group, so that the miR-29a-3p can be used as a marker for diagnosing the Graves disease. The miR-29a-3p disclosed by the invention not only can be used for rapidly and effectively diagnosing the Graves disease, but also provides a therapeutic target and an important basis for clinical applications such as gene therapy and drug therapy.

Description

Technical field [0001] The present invention belongs to the field of biomedical technology, and specific to the application of miR-29a-3p in the preparation of toxic diffuse thyroid diagnosis products. Background technique [0002] Graves' Disease, GD), but also a diffuse thyroid, which is a common disease-specific autoimmune disease, which is a major disease that endangers the health of my country. GD is one of the highest incidence of thyroid hyperthyroidism, accounting for 85%. The disease has a thyroxin receptor antibody in a patient to stimulate the thyroid-stim μlating hormonne (TSH) receptor on the thyroid-stim μlating hormone, TSH) receptor, causing excessive characteristics of thyroid hormone generation and release of thyroid hormone. The incidence of ordinary populations is about 0.5%, and women are significantly high (4 ~ 6: 1). GD can occur in each age stage, especially in 20 ~ 50. Its clinical manifestation is mainly divided into: (1) diffuse thyroid; (2) thyroidia; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/178C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 彭辉勇柳迎昭王雪华
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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