Construction method of fingerprint spectrum of clove and kaki calyx powder as well as standard fingerprint spectrum and application of fingerprint spectrum

A technology of standard fingerprints and construction methods, applied in the field of construction of clove persimmon powder fingerprints, can solve problems such as inability to guide, unclear stability of other substances, inability to control and quantify clove persimmon soup granules, etc., to achieve Good separation, good feasibility, stable baseline effect

Pending Publication Date: 2022-03-01
SHINEWAY PHARMA GRP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Liu Yinzhen of Guangdong Pharmaceutical University disclosed a method for constructing fingerprints and fingerprints of Clove Shidi Decoction Granules in his master's thesis "Research and Development of Clove Shidi Decoction Granules" published in 2017. It is based on acetonitrile ( A)-0.1wt% phosphoric acid aqueous solution (B) is mobile phase, utilizes high-performance liquid chromatography technique, has obtained the fingerprint collection of clove persimmon soup granule with 11 common characteristic peaks, but it only carries out these 11 characteristic peaks investigation, and the stability of the remaining substances is not clear, so it is impossible to carry out quality control and quantitative research on the whole recipe of Dingxiangshitang Granules
Moreover, the standard fingerprints disclosed in this document only have a certain guiding significance for Clove Shidi Granules, and cannot play an all-round guiding role for Clove Shidi Powder and other formulations involved in Clove Shiba Powder.

Method used

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  • Construction method of fingerprint spectrum of clove and kaki calyx powder as well as standard fingerprint spectrum and application of fingerprint spectrum
  • Construction method of fingerprint spectrum of clove and kaki calyx powder as well as standard fingerprint spectrum and application of fingerprint spectrum
  • Construction method of fingerprint spectrum of clove and kaki calyx powder as well as standard fingerprint spectrum and application of fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Embodiment 1 A kind of construction method of clove persimmon pedicle powder fingerprint

[0089] In this embodiment, the clove persimmon powder freeze-dried powder is used to construct the fingerprint of clove persimmon powder, and the preparation method of the clove persimmon powder freeze-dried powder is as follows:

[0090] Weigh 7.46g of cloves, 7.46g of persimmon stalks, 3.73g of ginseng, and 11.19g of ginger, add them to a decoction pot, add 300mL of pure water, soak for 30min, cover and decoct for 10min, stir three times with a glass rod, and filter while hot Mesh sieve, squeeze the filter residue. The decoction liquid was freeze-dried for 24-36 hours (temperature: -50°C, vacuum degree: 10Pa-20Pa), and ground through a 65-mesh sieve to obtain the freeze-dried powder of clove persimmon root powder.

[0091] The present embodiment is a method for constructing a fingerprint of clove persimmon stalks, comprising the following specific steps:

[0092] 1) Preparatio...

Embodiment 2～5

[0115] The construction method of embodiment 2~5 clove persimmon pedicle powder fingerprint

[0116] Embodiments 2 to 5 are respectively a method for constructing fingerprints of clove persimmon pedicle powder, and their steps are basically the same as in Example 1, the only difference being that the process parameters are different, see Table 1 for details:

[0117] List of each technological parameter in the embodiment 2~5 of table 1

[0118]

[0119] The contents of the other parts of Embodiments 2 to 5 are the same as those of Embodiment 1.

Embodiment 6

[0120] The standard fingerprint spectrum of embodiment 6 clove persimmon root powder

[0121] Using the method for constructing the fingerprints of clove persimmon powder in Example 1, measure the fingerprints of 17 batches of clove persimmon powder freeze-dried powder under 203nm wavelength conditions, see figure 1 , the retention time of each peak is shown in Table 2 in the fingerprint of each batch of clove persimmon powder freeze-dried powder obtained, and the relative retention time of each peak is shown in Table 3;

[0122] Using the National Pharmacopoeia Committee "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2004 Edition)" to synthesize the fingerprints of the above-mentioned 17 batches of clove persimmon powder, generate a standard of clove persimmon powder under the condition of 203nm wavelength composed of 38 characteristic peaks Fingerprint, see figure 2 . Wherein, taking No. 4 peak gallic acid as the reference peak, the relative r...

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Abstract

The invention provides a construction method of a fingerprint spectrum of syzygium aromaticum and kaki calyx powder as well as a standard fingerprint spectrum and application thereof, and belongs to the technical field of drug detection.The construction method comprises the steps that a test solution and a reference solution are prepared firstly, then acetonitrile (A)-0.02 wt% phosphoric acid aqueous solution (B) is used as a mobile phase, and the standard fingerprint spectrum of the syzygium aromaticum and kaki calyx powder is constructed. Respectively carrying out high performance liquid chromatography detection on the test solution and the reference substance solution to obtain a fingerprint spectrum of the clove and persimmon calyx powder; the standard fingerprint spectrum of the clove and kaki calyx powder is obtained by using the construction method and is used for quality evaluation or control in the whole process of research / development / production / clinical application of the clove and kaki calyx powder. According to the construction method of the fingerprint spectrum of the clove and kaki calyx powder, all medicinal material components in the whole formula of the clove and kaki calyx powder can be detected together; the provided standard fingerprint spectrum can provide a scientific basis for comprehensively establishing a quality control standard of the clove and kaki calyx powder.

Description

technical field [0001] The invention relates to a drug detection technology, in particular to a method for constructing a clove persimmon stalk fingerprint and its standard fingerprint and application. Background technique [0002] Dingxiang Shidi San is the name of a traditional Chinese medicine prescription, which comes from Yang Lishan's "Treatise on Febrile Diseases" in the Qing Dynasty. It contains cloves, persimmon pedicles, ginger and ginseng. Hiccups caused by cold, stomach loss and descending, and qi inversion. At the same time, studies have shown that Clove Shidi Powder has a very positive effect on cardiovascular and blood rheology, and also has certain anti-inflammatory, anti-oxidative damage and anti-pathogenic microorganism effects. [0003] Because the requirements of the international community for medicines are "safe, effective and controllable", in order to study and develop clove persimmon powder, it is necessary to study its quality control. In the curr...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686G01N2030/047Y02A50/30
Inventor 张燕玲李寅庆侯金才孙长清赵亚楠李英丽蔡向杰
Owner SHINEWAY PHARMA GRP LTD
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