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Application of tea saponin in preparation of medicine for treating shigella infectious enteritis and medicine preparation of tea saponin

A pharmaceutical preparation, shigella technology, applied in the field of biomedicine, can solve problems such as application has not been reported in the literature, achieve the effect of restoring integrity, good application prospects, and protecting the intestinal tract

Pending Publication Date: 2022-03-04
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Tea saponin (tea saponin, TS), as an important bioactive component in tea extract, is chemically a kind of oleanane-type pentacyclic triterpenoid saponin mixture, and its unique structure makes it have special biological properties. Active, has biological activities such as anti-osmotic anti-inflammatory, anti-bacterial, inhibition of ethanol absorption, anti-hypertension, anti-oxidation, etc. It is widely used in agriculture, daily chemicals, building materials and other fields. Although it is involved in the field of medicine, tea saponin is improving There is no literature report on the application of Shigella infectious enteritis

Method used

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  • Application of tea saponin in preparation of medicine for treating shigella infectious enteritis and medicine preparation of tea saponin
  • Application of tea saponin in preparation of medicine for treating shigella infectious enteritis and medicine preparation of tea saponin
  • Application of tea saponin in preparation of medicine for treating shigella infectious enteritis and medicine preparation of tea saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In vitro Inhibitory Experiment of Tea Saponin on Shigella

[0025] 1. Experimental steps

[0026] The tea saponin in vitro antibacterial experiment involved in the following examples all adopts the following process:

[0027] Experimental materials: tea saponin was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (S299339, purity ≥ 60%, extracted from tea tree seeds);

[0028] The strain of Shigella flexneri is Shigella flexneri 2a 301 strain (sf301, which has been used as a reference strain of Shigella flexneri in China);

[0029] Clinical drug-resistant Shigella flexneri 2448 (RF-2448) and clinical drug-resistant Shigella flexneri II-1 (RF-II-1) were provided by the Affiliated Hospital of Xuzhou Medical University, and their drug sensitivity results are shown in Table 1 , Table 2; It can be seen from the table that RF-2448 and RF-II-1 are resistant to most antibiotics. Through the antibacterial experiment of TS on these two clinical Shigella, explore...

Embodiment 2

[0050] Therapeutic Effect of Tea Saponin on Shigella Infectious Enteritis

[0051] 1. Experimental steps

[0052] The establishment of the Shigella-induced mouse colitis model involved in the following examples all adopts the following process:

[0053] Experimental animals: male C57BL / 6 mice, weighing 17-18 g, specific pathogen-free (SPF), provided by Zhejiang Weitong Lihua Company. The mice were raised in the Experimental Animal Center of Xuzhou Medical University. The room temperature was 25°C and the humidity was 40-70%. They were given 12 hours of day and night alternating light source, standard laboratory sterilized feed, free to eat and drink, and adapted to the environment for three days before the experiment.

[0054] Such as image 3 As shown, mice were divided into random control group (NC), Shigella infection group (Sf301), and tea saponin treatment group (TS), 5 mice in each group. In the NC group, 200 μL of sterile water was administered to each mouse per day ...

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Abstract

The invention discloses application of tea saponin in preparation of a medicine for treating shigella infectious enteritis and a medicine preparation of the tea saponin, and belongs to the field of biological medicine. Experiments show that in vitro, the bacteriostatic action of the tea saponin (TS) is related to the concentration of the TS, and the higher the concentration of the TS is, the more obvious the bacteriostatic action of the TS is. In vivo, TS can relieve colon injury; through H-E staining and bacterial loading amount detection, TS can protect intestinal mucosa epithelial tissue, significantly reduce shigella cecum loading amount, significantly relieve colon tissue damage caused by shigella infection, and improve a colon mucus barrier function; real-time fluorescent quantitative PCR (RT-qPCR) detection shows that TS significantly reduces the expression level of proinflammatory factors and significantly increases the expression level of anti-inflammatory factors at the same time, so that intestinal inflammation is relieved. The invention provides important data for developing a medicinal preparation aiming at shigella infectious enteritis.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of tea saponin in the preparation of medicines for treating Shigella infectious enteritis and its pharmaceutical preparations. Background technique [0002] Shigella flexneri is a gram-negative bacillus that commonly causes bacillary dysentery characterized by acute mucoid inflammation. It is estimated that more than 165 million cases of shigellosis are reported each year, and about 1 million people die from shigellosis in the world every year. This disease has become a huge global public health problem, especially in developing countries and low-income countries. In the absence of effective treatment, patients with shigellosis may develop secondary complications such as sepsis, pneumonia, and hemolytic uremic syndrome. [0003] Shigella can easily induce intestinal inflammation, especially colitis. Shigella mainly invades and destroys colonic epithelium th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K36/82A61P31/04A61P1/00
CPCA61K31/704A61K36/82A61P31/04A61P1/00Y02A50/30
Inventor 杨欢单云洁吴小小
Owner XUZHOU MEDICAL UNIV